Methods and Compositions for Weed Control

ABSTRACT

Novel compositions for use to enhance weed control. Specifically, the present invention provides for methods and compositions that modulate protoporphyrinogen IX oxidase in weed species. The present invention also provides for combinations of compositions and methods that enhance weed control.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of U.S. application Ser. No.13/612,985 filed on Sep. 13, 2012, which claims the benefit and priorityof U.S. Provisional Patent Application No. 61/534,086 filed on Sep. 13,2011, each of which is herein incorporated by reference in its entirety.The sequence listing that is contained in the file named“P34115US02_Sequence_listing_ST25.txt,” which is 887,739 bytes (measuredin operating system MS-Windows) and was created on Jul. 21, 2016, isfiled herewith and incorporated herein by reference.

FIELD

The methods and compositions generally relate to the field of weedmanagement. More specifically, the methods and compositions relate toprotoporphyrinogen IX oxidase (PPG oxidase) genes in plants andcompositions containing polynucleotide molecules for modulating and/orregulating their expression. Further provided are methods andcompositions useful for weed control.

BACKGROUND

Weeds are plants that compete with cultivated plants in an agronomicenvironment and cost farmers billions of dollars annually in crop lossesand the expense of efforts to keep weeds under control. Weeds also serveas hosts for crop diseases and insect pests. The losses caused by weedsin agricultural production environments include decreases in crop yield,reduced crop quality, increased irrigation costs, increased harvestingcosts, reduced land value, injury to livestock, and crop damage frominsects and diseases harbored by the weeds. The principal means by whichweeds cause these effects are: 1) competing with crop plants for water,nutrients, sunlight and other essentials for growth and development, 2)production of toxic or irritant chemicals that cause human or animalhealth problem, 3) production of immense quantities of seed orvegetative reproductive parts or both that contaminate agriculturalproducts and perpetuate the species in agricultural lands, and 4)production on agricultural and nonagricultural lands of vast amounts ofvegetation that must be disposed of Herbicide tolerant weeds are aproblem with nearly all herbicides in use, there is a need toeffectively manage these weeds. There are over 365 weed biotypescurrently identified as being herbicide resistant to one or moreherbicides by the Herbicide Resistance Action Committee (HRAC), theNorth American Herbicide Resistance Action Committee (NAHRAC), and theWeed Science Society of America (WSSA).

BRIEF DESCRIPTION OF THE FIGURES

The following drawings form part of the present specification and areincluded to further demonstrate certain methods, compositions orresults. They may be better understood by reference to one or more ofthese drawings in combination with the detailed description of specificembodiments presented herein. The invention can be more fully understoodfrom the following description of the figures:

FIG. 1. Treatment of Amaranthus palmeri with ssDNA triggerpolynucleotides and PPG oxidase inhibitor herbicide, flumioxazin.

FIG. 2. Treatment of Amaranthus palmeri with ssDNA triggerpolynucleotides and PPG oxidase inhibitor herbicide, fomesafen.

FIG. 3. Treatment of Amaranthus palmeri with pooled oligos and ssDNAtrigger polynucleotides and PPG oxidase inhibitor herbicide, Reflex®(fomesafen).

SUMMARY

In one aspect, the invention provides a method of plant controlcomprising an external application to a plant of a compositioncomprising a polynucleotide and a transfer agent, wherein thepolynucleotide is essentially identical or essentially complementary toa PPG oxidase gene sequence or fragment thereof, or to the RNAtranscript of said PPG oxidase gene sequence or fragment thereof,wherein said PPG oxidase gene sequence is selected from the groupconsisting of SEQ ID NOs:1-71 and a polynucleotide fragment thereof,whereby the plant growth or development or reproductive ability isreduced or the weedy plant is made more sensitive to a PPG oxidaseinhibitor herbicide relative to a plant not treated with saidcomposition. In this manner, plants that have become resistant to theapplication of PPG oxidase inhibitor containing herbicides may be mademore susceptible to the herbicidal effects of a PPG oxidase inhibitorcontaining herbicide, thus potentiating the effect of the herbicide. Thepolynucleotide fragment is at least 18 contiguous nucleotides, at least19 contiguous nucleotides, at least 20 contiguous nucleotides or atleast 21 contiguous nucleotides in length and at least 85 percentidentical to a PPG oxidase gene sequence selected from the groupconsisting of SEQ ID NOs:1-71 and the transfer agent is anorganosilicone composition or compound. The polynucleotide fragment canalso be sense or anti-sense ssDNA or ssRNA, dsRNA or dsDNA, or dsDNA/RNAhybrids. The composition can include more than one polynucleotidefragments, and the composition can include a PPG oxidase inhibitorherbicide and/or other herbicides that enhance the weed control activityof the composition.

In another aspect, polynucleotide molecules and methods for modulatingPPG oxidase gene expression in weedy plant species are provided. Themethod reduces, represses or otherwise delays expression of a PPGoxidase gene in a weedy plant comprising an external application to aweedy plant of a composition comprising a polynucleotide and a transferagent, wherein the polynucleotide is essentially identical oressentially complementary to a PPG oxidase gene sequence or fragmentthereof, or to the RNA transcript of the PPG oxidase gene sequence orfragment thereof, wherein the PPG oxidase gene sequence is selected fromthe group consisting of SEQ ID NOs:1-71 and a polynucleotide fragmentthereof. The polynucleotide fragment is at least 18 contiguousnucleotides, at least 19 contiguous nucleotides, at least 20 contiguousnucleotides, at least 21 contiguous nucleotides in length and at least85 percent identical to a PPG oxidase gene sequence selected from thegroup consisting of SEQ ID NOs:1-71 and the transfer agent is anorganosilicone compound. The polynucleotide fragment can also be senseor anti-sense ssDNA or ssRNA, dsRNA or dsDNA, or dsDNA/RNA hybrids.

In a further aspect, the polynucleotide molecule containing compositionmay be combined with other herbicidal compounds to provide additionalcontrol of unwanted plants in a field of cultivated plants.

In a further aspect, the polynucleotide molecule composition may becombined with any one or more additional agricultural chemicals, such asinsecticides, fungicides, nematocides, bactericides, acaricides, growthregulators, chemosterilants, semiochemicals, repellents, attractants,pheromones, feeding stimulants, biopesticides, microbial pesticides orother biologically active compounds to form a multi-component pesticidegiving an even broader spectrum of agricultural protection.

DETAILED DESCRIPTION

Provided are methods and compositions containing a polynucleotide thatprovide for regulation, repression or delay of PPG oxidase(protoporphyrinogen IX oxidase) gene expression and enhanced control ofweedy plant species and importantly PPG oxidase inhibitor resistant weedbiotypes. Aspects of the method can be applied to manage various weedyplants in agronomic and other cultivated environments.

The following definitions and methods are provided to better define thepresent invention and to guide those of ordinary skill in the art in thepractice of the present invention. Unless otherwise noted, terms are tobe understood according to conventional usage by those of ordinary skillin the relevant art. Where a term is provided in the singular, theinventors also contemplate aspects of the invention described by theplural of that term.

By “non-transcribable” polynucleotides is meant that the polynucleotidesdo not comprise a complete polymerase II transcription unit.

As used herein “solution” refers to homogeneous mixtures andnon-homogeneous mixtures such as suspensions, colloids, micelles, andemulsions.

Weedy plants are plants that compete with cultivated plants, those ofparticular importance include, but are not limited to, importantinvasive and noxious weeds and herbicide resistant biotypes in cropproduction, such as Amaranthus species—A. albus, A. blitoides, A.hybridus, A. palmeri, A. powellii, A. retroflexus, A. spinosus, A.tuberculatus, and A. viridis; Ambrosia species—A. trifida, A.artemisifolia; Lolium species—L. multiflorum, L. rigidium, L perenne;Digitaria species—D. insularis; Euphorbia species—E. heterophylla;Kochia species—K. scoparia; Sorghum species—S. halepense; Conyzaspecies—C. bonariensis, C. canadensis, C. sumatrensis; Chlorisspecies—C. truncate; Echinochloa species—E. colona, E. crus-galli;Eleusine species—E. indica; Poa species—P. annua; Plantago species—P.lanceolata; Avena species—A. fatua; Chenopodium species—C. album;Setaria species—S. viridis, Abutilon theophrasti; Ipomoea species;Sesbania species; Cassia species; Sida species; Brachiaria species; andSolanum species.

Additional weedy plant species found in cultivated areas includeAlopecurus myosuroides, Avena sterilis, Avena sterilis ludoviciana,Brachiaria plantaginea, Bromus diandrus, Bromus rigidus, Cynosurusechinatus, Digitaria ciliaris, Digitaria ischaemum, Digitariasanguinalis, Echinochloa oryzicola, Echinochloa phyllopogon, Eriochloapunctata, Hordeum glaucum, Hordeum leporinum, Ischaemum rugosum,Leptochloa chinensis, Lolium persicum, Phalaris minor, Phalarisparadoxa, Rottboellia exalta, Setaria faberi, Setaria viridis var,robusta-alba schreiber, Setaria viridis var, robusta-purpurea, Snowdeniapolystachea, Sorghum sudanese, Alisma plantago-aquatica, Amaranthuslividus, Amaranthus quitensis, Ammania auriculata, Ammania coccinea,Anthemis cotula, Apera spica-venti, Bacopa rotundifolia, Bidens pilosa,Bidens subalternans, Brassica tournefortii, Bromus tectorum, Camelinamicrocarpa, Chrysanthemum coronarium, Cuscuta campestris, Cyperusdifformis, Damasonium minus, Descurainia sophia, Diplotaxis tenuifolia,Echium plantagineum, Elatine triandra var, pedicellata, Euphorbiaheterophylla, Fallopia convolvulus, Fimbristylis miliacea, Galeopsistetrahit, Galium spurium, Helianthus annuus, Iva xanthifolia, Ixophorusunisetus, Ipomoea indica, Ipomoea purpurea, Ipomoea sepiaria, Ipomoeaaquatic, Ipomoea triloba, Lactuca serriola, Limnocharis flava,Limnophila erecta, Limnophila sessiliflora, Lindernia dubia, Linderniadubia var major, Lindernia micrantha, Lindernia procumbens,Mesembryanthemum crystallinum, Monochoria korsakowii, Monochoriavaginalis, Neslia paniculata, Papaver rhoeas, Parthenium hysterophorus,Pentzia suffruticosa, Phalaris minor, Raphanus raphanistrum, Raphanussativus, Rapistrum rugosum, Rotala indica var, uliginosa, Sagittariaguyanensis, Sagittaria montevidensis, Sagittaria pygmaea, Salsolaiberica, Scirpus juncoides var ohwianus, Scirpus mucronatus, Setarialutescens, Sida spinosa, Sinapis arvensis, Sisymbrium orientale,Sisymbrium thellungii, Solanum ptycanthum, Sonchus aspen, Sonchusoleraceus, Sorghum bicolor, Stellaria media, Thlaspi arvense, Xanthiumstrumarium, Arctotheca calendula, Conyza sumatrensis, Crassocephalumcrepidiodes, Cuphea carthagenenis, Epilobium adenocaulon, Erigeronphiladelphicus, Landoltia punctata, Lepidium virginicum, Monochoriakorsakowii, Solanum americanum, Solanum nigrum, Vulpia bromoides,Youngia japonica, Hydrilla verticillata, Carduus nutans, Carduuspycnocephalus, Centaurea solstitialis, Cirsium arvense, Commelinadiffusa, Convolvulus arvensis, Daucus carota, Digitaria ischaemum,Echinochloa crus-pavonis, Fimbristylis miliacea, Galeopsis tetrahit,Galium spurium, Limnophila erecta, Matricaria perforate, Papaver rhoeas,Ranunculus acris, Soliva sessilis, Sphenoclea zeylanica, Stellariamedia, Nassella trichotoma, Stipa neesiana, Agrostis stolonifera,Polygonum aviculare, Alopecurus japonicus, Beckmannia syzigachne, Bromustectorum, Chloris inflate, Echinochloa erecta, Portulaca oleracea, andSenecio vulgaris. It is believed that all plants contain aprotoporphyrinogen IX oxidase gene in their genome, the sequence ofwhich can be isolated, and polynucleotides made according to the methodsof the present invention that are useful for regulating, suppressing ordelaying the expression of the target PPG oxidase gene in the plants andthe growth or development of the treated plants.

Some cultivated plants may also be weedy plants when they occur inunwanted environments. Transgenic crops with one or more herbicidetolerances will need specialized methods of management to control weedsand volunteer crop plants and to target the herbicide tolerancetransgene as necessary to permit the treated plants to become sensitiveto the herbicide.

A “trigger” or “trigger polynucleotide” is a polynucleotide moleculethat is homologous or complementary to a target gene polynucleotide. Thetrigger polynucleotide molecules modulate expression of the target genewhen topically applied to a plant surface with a transfer agent, wherebya plant treated with said composition has its growth or development orreproductive ability regulated, suppressed or delayed or said plant ismore sensitive to a PPG oxidase inhibitor herbicide or mitosis inhibitorherbicide as a result of said polynucleotide containing compositionrelative to a plant not treated with a composition containing thetrigger molecule. Trigger polynucleotides disclosed herein are generallydescribed in relation to the target gene sequence and may be used in thesense (homologous) or antisense (complementary) orientation as singlestranded molecules or comprise both strands as double stranded moleculesor nucleotide variants and modified nucleotides thereof depending on thevarious regions of a gene being targeted.

It is contemplated that the composition of the present invention willcontain multiple polynucleotides and herbicides that include but are notlimited to PPG oxidase gene trigger polynucleotides and a PPG oxidaseinhibitor herbicide and any one or more additional herbicide target genetrigger polynucleotides and the related herbicides and any one or moreadditional essential gene trigger polynucleotides. Essential genes aregenes in a plant that provide key enzymes or other proteins, forexample, a biosynthetic enzyme, metabolizing enzyme, receptor, signaltransduction protein, structural gene product, transcription factor, ortransport protein; or regulating RNAs, such as microRNAs, that areessential to the growth or survival of the organism or cell or involvedin the normal growth and development of the plant (Meinke et al., TrendsPlant Sci., 2008 September; 13(9):483-91). The suppression of anessential gene enhances the effect of a herbicide that affects thefunction of a gene product different than the suppressed essential gene.The compositions of the present invention can include various triggerpolynucleotides that modulate the expression of an essential gene otherthan PPG oxidase.

Herbicides for which transgenes for plant tolerance have beendemonstrated and the method of the present invention can be applied,include but are not limited to: auxin-like herbicides, glyphosate,glufosinate, sulfonylureas, imidazolinones, bromoxynil, delapon,dicamba, cyclohezanedione, protoporphyrinogen oxidase inhibitors,4-hydroxyphenyl-pyruvate-dioxygenase inhibitors herbicides. For example,transgenes and their polynucleotide molecules that encode proteinsinvolved in herbicide tolerance are known in the art, and include, butare not limited to a 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS), for example, as more fully described in U.S. Pat. No. 7,807,791(SEQ ID NO:5); U.S. Pat. Nos. 6,248,876 B1; 5,627,061; 5,804,425;5,633,435; 5,145,783; 4,971,908; 5,462,910; 5,188,642; 4,940,835;5,866,775; 6,225,114 B1; 6,130,366; 5,460,667; 4,535,060; 4,769,061;5,633,448; 5,510,471; Re. 36,449; Re. 37,287 E; and 5,491,288; toleranceto sulfonylurea and/or imidazolinone, for example, as described morefully in U.S. Pat. Nos. 5,605,011; 5,013,659; 5,141,870; 5,767,361;5,746,180; 5,304,732; 4,761,373; 5,346,107; 5,928,937; and 5,378,824;and international publication WO 96/33270; tolerance tohydroxyphenylpyruvatedioxygenases inhibiting herbicides in plants aredescribed in U.S. Pat. Nos. 6,245,968 B1; 6,268,549; and U.S. Pat. Nos.6,069,115; 7,462,379 (SEQ ID NO:3); U.S. Pat. Nos. 7,935,869; 7,304,209(SEQ ID NOs:1, 3, 5 and 15); and US Pat. Pub. 20110191897;aryloxyalkanoate dioxygenase polynucleotides, which confer tolerance to2,4-D and other phenoxy auxin herbicides as well as toaryloxyphenoxypropionate herbicides as described, for example, inWO2005/107437; U.S. Pat. No. 7,838,733 (SEQ ID NO:5); anddicamba-tolerance polynucleotides as described, for example, in Hermanet al. (2005), J. Biol. Chem., 280: 24759-24767. Other examples ofherbicide-tolerance traits include those conferred by polynucleotidesencoding an exogenous phosphinothricin acetyltransferase, as describedin U.S. Pat. Nos. 5,969,213; 5,489,520; 5,550,468; 5,874,265; 5,919,675;5,561,236; 5,648,477; 5,646,024; 6,177,616; and 5,879,903. Plantscontaining an exogenous phosphinothricin acetyltransferase can exhibitimproved tolerance to glufosinate herbicides, which inhibit the enzymeglutamine synthase. Additionally, herbicide-tolerance polynucleotidesinclude those conferred by polynucleotides conferring alteredprotoporphyrinogen oxidase (protox) activity, as described in U.S. Pat.Nos. 6,288,306 B1; 6,282,837 B1; and 5,767,373; and WO 01/12825. Plantscontaining such polynucleotides can exhibit improved tolerance to any ofa variety of herbicides which target the protox enzyme (also referred toas protox inhibitors). Polynucleotides encoding a glyphosateoxidoreductase and a glyphosate-N-acetyl transferase (GOX described inU.S. Pat. No. 5,463,175 and GAT described in U.S. Patent publication20030083480, dicamba monooxygenase described in U.S. Patent publication20030135879, all of which are incorporated herein by reference); apolynucleotide molecule encoding bromoxynil nitrilase (Bxn described inU.S. Pat. No. 4,810,648 for Bromoxynil tolerance, which is incorporatedherein by reference); a polynucleotide molecule encoding phytoenedesaturase (crtI) described in Misawa et al. (1993), Plant J.,4:833-840, and Misawa et al. (1994), Plant J., 6:481-489, fornorflurazon tolerance; a polynucleotide molecule encodingacetohydroxyacid synthase (AHAS, aka ALS) described in Sathasiivan etal. (1990), Nucl. Acids Res., 18:468-2193, for tolerance to sulfonylureaherbicides; and the bar gene described in DeBlock et al. (1987), EMBOJ., 6:2513-2519, for glufosinate and bialaphos tolerance. The transgeniccoding regions and regulatory elements of the herbicide tolerance genesare targets in which polynucleotide triggers and herbicides can beincluded in the composition of the present invention.

The composition includes a component that is a PPG oxidase inhibitorherbicide, which includes but is not limited to acifluorfen-Na, bifenox,chlomethoxyfen, fluoroglycofen-ethyl, fomesafen, halosafen, lactofen,oxyfluorfen, fluazolate, pyraflufen-ethyl, cinidon-ethyl, flumioxazin,flumiclorac-pentyl, fluthiacet-methyl, thidiazimin, oxadiazon,oxadiargyl, azafenidin, carfentrazone-ethyl, sulfentrazone, pentoxazone,benzfendizone, butafenacil, pyrazogyl, and profluazol.

Numerous additional herbicides with similar or different modes of action(herein referred to as co-herbicides) are available that can be added tothe composition, for example, members of the herbicide families thatinclude but are not limited to amide herbicides, aromatic acidherbicides, arsenical herbicides, benzothiazole herbicides,benzoylcyclohexanedione herbicides, benzofuranyl alkylsulfonateherbicides, carbamate herbicides, cyclohexene oxime herbicides,cyclopropylisoxazole herbicides, dicarboximide herbicides,dinitroaniline herbicides, dinitrophenol herbicides, diphenyl etherherbicides, dithiocarbamate herbicides, halogenated aliphaticherbicides, imidazolinone herbicides, inorganic herbicides, nitrileherbicides, organophosphorus herbicides, oxadiazolone herbicides,oxazole herbicides, phenoxy herbicides, phenylenediamine herbicides,pyrazole herbicides, pyridazine herbicides, pyridazinone herbicides,pyridine herbicides, pyrimidinediamine herbicides,pyrimidinyloxybenzylamine herbicides, quaternary ammonium herbicides,thiocarbamate herbicides, thiocarbonate herbicides, thiourea herbicides,triazine herbicides, triazinone herbicides, triazole herbicides,triazolone herbicides, triazolopyrimidine herbicides, uracil herbicides,and urea herbicides. In particular, the rates of use of the addedherbicides can be reduced in compositions comprising thepolynucleotides. Contemplated use rate reductions of the additionaladded herbicides can be 10-25 percent, 26-50 percent, 51-75 percent ormore, and can be achieved from the enhanced activity of thepolynucleotides and herbicide composition. Representative co-herbicidesof the families include but are not limited to acetochlor, acifluorfen,acifluorfen-sodium, aclonifen, acrolein, alachlor, alloxydim, allylalcohol, ametryn, amicarbazone, amidosulfuron, aminopyralid, amitrole,ammonium sulfamate, anilofos, asulam, atraton, atrazine, azimsulfuron,BCPC, beflubutamid, benazolin, benfluralin, benfuresate, bensulfuron,bensulfuron-methyl, bensulide, bentazone, benzfendizone, benzobicyclon,benzofenap, bifenox, bilanafos, bispyribac, bispyribac-sodium, borax,bromacil, bromobutide, bromoxynil, butachlor, butafenacil, butamifos,butralin, butroxydim, butylate, cacodylic acid, calcium chlorate,cafenstrole, carbetamide, carfentrazone, carfentrazone-ethyl, CDEA,CEPC, chlorflurenol, chlorflurenol-methyl, chloridazon, chlorimuron,chlorimuron-ethyl, chloroacetic acid, chlorotoluron, chlorpropham,chlorsulfuron, chlorthal, chlorthal-dimethyl, cinidon-ethyl,cinmethylin, cinosulfuron, cisanilide, clethodim, clodinafop,clodinafop-propargyl, clomazone, clomeprop, clopyralid, cloransulam,cloransulam-methyl, CMA, 4-CPB, CPMF, 4-CPP, CPPC, cresol, cumyluron,cyanamide, cyanazine, cycloate, cyclosulfamuron, cycloxydim, cyhalofop,cyhalofop-butyl, 2,4-D, 3,4-DA, daimuron, dalapon, dazomet, 2,4-DB,3,4-DB, 2,4-DEB, desmedipham, dicamba, dichlobenil,ortho-dichlorobenzene, para-dichlorobenzene, dichlorprop, dichlorprop-P,diclofop, diclofop-methyl, diclosulam, difenzoquat, difenzoquatmetilsulfate, diflufenican, diflufenzopyr, dimefuron, dimepiperate,dimethachlor, dimethametryn, dimethenamid, dimethenamid-P, dimethipin,dimethylarsinic acid, dinitramine, dinoterb, diphenamid, diquat, diquatdibromide, dithiopyr, diuron, DNOC, 3,4-DP, DSMA, EBEP, endothal, EPTC,esprocarb, ethalfluralin, ethametsulfuron, ethametsulfuron-methyl,ethofumesate, ethoxyfen, ethoxysulfuron, etobenzanid, fenoxaprop-P,fenoxaprop-P-ethyl, fentrazamide, ferrous sulfate, flamprop-M,flazasulfuron, florasulam, fluazifop, fluazifop-butyl, fluazifop-P,fluazifop-P-butyl, flucarbazone, flucarbazone-sodium, flucetosulfuron,fluchloralin, flufenacet, flufenpyr, flufenpyr-ethyl, flumetsulam,flumiclorac, flumiclorac-pentyl, flumioxazin, fluometuron,fluoroglycofen, fluoroglycofen-ethyl, flupropanate, flupyrsulfuron,flupyrsulfuron-methyl-sodium, flurenol, fluridone, fluorochloridone,fluoroxypyr, flurtamone, fluthiacet, fluthiacet-methyl, fomesafen,foramsulfuron, fosamine, glufosinate, glufosinate-ammonium, glyphosate,halosulfuron, halosulfuron-methyl, haloxyfop, haloxyfop-P, HC-252,hexazinone, imazamethabenz, imazamethabenz-methyl, imazamox, imazapic,imazapyr, imazaquin, imazethapyr, imazosulfuron, indanofan, iodomethane,iodosulfuron, iodosulfuron-methyl-sodium, ioxynil, isoproturon, isouron,isoxaben, isoxachlortole, isoxaflutole, karbutilate, lactofen, lenacil,linuron, MAA, MAMA, MCPA, MCPA-thioethyl, MCPB, mecoprop, mecoprop-P,mefenacet, mefluidide, mesosulfuron, mesosulfuron-methyl, mesotrione,metam, metamifop, metamitron, metazachlor, methabenzthiazuron,methylarsonic acid, methyldymron, methyl isothiocyanate, metobenzuron,metolachlor, S-metolachlor, metosulam, metoxuron, metribuzin,metsulfuron, metsulfuron-methyl, MK-66, molinate, monolinuron, MSMA,naproanilide, napropamide, naptalam, neburon, nicosulfuron, nonanoicacid, norflurazon, oleic acid (fatty acids), orbencarb, orthosulfamuron,oryzalin, oxadiargyl, oxadiazon, oxasulfuron, oxaziclomefone,oxyfluorfen, paraquat, paraquat dichloride, pebulate, pendimethalin,penoxsulam, pentachlorophenol, pentanochlor, pentoxazone, pethoxamid,petrolium oils, phenmedipham, phenmedipham-ethyl, picloram, picolinafen,pinoxaden, piperophos, potassium arsenite, potassium azide,pretilachlor, primisulfuron, primisulfuron-methyl, prodiamine,profluazol, profoxydim, prometon, prometryn, propachlor, propanil,propaquizafop, propazine, propham, propisochlor, propoxycarbazone,propoxycarbazone-sodium, propyzamide, prosulfocarb, prosulfuron,pyraclonil, pyraflufen, pyraflufen-ethyl, pyrazolynate, pyrazosulfuron,pyrazosulfuron-ethyl, pyrazoxyfen, pyribenzoxim, pyributicarb,pyridafol, pyridate, pyriftalid, pyriminobac, pyriminobac-methyl,pyrimisulfan, pyrithiobac, pyrithiobac-sodium, quinclorac, quinmerac,quinoclamine, quizalofop, quizalofop-P, rimsulfuron, sethoxydim,siduron, simazine, simetryn, SMA, sodium arsenite, sodium azide, sodiumchlorate, sulcotrione, sulfentrazone, sulfometuron, sulfometuron-methyl,sulfosate, sulfosulfuron, sulfuric acid, tar oils, 2,3,6-TBA, TCA,TCA-sodium, tebuthiuron, tepraloxydim, terbacil, terbumeton,terbuthylazine, terbutryn, thenylchlor, thiazopyr, thifensulfuron,thifensulfuron-methyl, thiobencarb, tiocarbazil, topramezone,tralkoxydim, tri-allate, triasulfuron, triaziflam, tribenuron,tribenuron-methyl, tricamba, triclopyr, trietazine, trifloxysulfuron,trifloxysulfuron-sodium, trifluralin, triflusulfuron,triflusulfuron-methyl, trihydroxytriazine, tritosulfuron,[3-[2-chloro-4-fluoro-5-(methyl-6-trifluoromethyl-2,4-dioxo-2,3,4-tetrahydropyrimidin-3-yl)phenoxy]-2-pyridyloxy]aceticacid ethyl ester (CAS RN 353292-3-6),4-[(4,5-dihydro-3-methoxy-4-methyl-5-oxo)-H-,2,4-triazolylcarbonyl-sulfamoyl]-5-methylthiophene-3-carboxylicacid (BAY636), BAY747 (CAS RN 33504-84-2), topramezone (CAS RN2063-68-8),4-hydroxy-3-[[2-[(2-methoxyethoxy)methyl]-6-(trifluoro-methyl)-3-pyridinyl]carbonyl]-bicyclo[3.2.]oct-3-en-2-one(CAS RN 35200-68-5), and4-hydroxy-3-[[2-(3-methoxypropyl)-6-(difluoromethyl)-3-pyridinyl]carbonyl]-bicyclo[3.2.]oct-3-en-2-one.Additionally, including herbicidal compounds of unspecified modes ofaction as described in CN101279950A, CN101279951A, DE10000600A1,DE10116399A1, DE102004054666A1, DE102005014638A1, DE102005014906A1,DE102007012168A1, DE102010042866A1, DE10204951A1, DE10234875A1,DE10234876A1, DE10256353A1, DE10256354A1, DE10256367A1, EP1157991A2,EP1238586A1, EP2147919A1, EP2160098A2, JP03968012B2, JP2001253874A,JP2002080454A, JP2002138075A, JP2002145707A, JP2002220389A,JP2003064059A, JP2003096059A, JP2004051628A, JP2004107228A,JP2005008583A, JP2005239675A, JP2005314407A, JP2006232824A,JP2006282552A, JP2007153847A, JP2007161701A, JP2007182404A,JP2008074840A, JP2008074841A, JP2008133207A, JP2008133218A,JP2008169121A, JP2009067739A, JP2009114128A, JP2009126792A,JP2009137851A, US20060111241A1, US20090036311A1, US20090054240A1,US20090215628A1, US20100099561A1, US20100152443A1, US20110105329A1,US20110201501A1, WO2001055066A2, WO2001056975A1, WO2001056979A1,WO2001090071A2, WO2001090080A1, WO2002002540A1, WO2002028182A1,WO2002040473A1, WO2002044173A2, WO2003000679A2, WO2003006422A1,WO2003013247A1, WO2003016308A1, WO2003020704A1, WO2003022051A1,WO2003022831A1, WO2003022843A1, WO2003029243A2, WO2003037085A1,WO2003037878A1, WO2003045878A2, WO2003050087A2, WO2003051823A1,WO2003051824A1, WO2003051846A2, WO2003076409A1, WO2003087067A1,WO2003090539A1, WO2003091217A1, WO2003093269A2, WO2003104206A2,WO2004002947A1, WO2004002981A2, WO2004011429A1, WO2004029060A1,WO2004035545A2, WO2004035563A1, WO2004035564A1, WO2004037787A1,WO2004067518A1, WO2004067527A1, WO2004077950A1, WO2005000824A1,WO2005007627A1, WO2005040152A1, WO2005047233A1, WO2005047281A1,WO2005061443A2, WO2005061464A1, WO2005068434A1, WO2005070889A1,WO2005089551A1, WO2005095335A1, WO2006006569A1, WO2006024820A1,WO2006029828A1, WO2006029829A1, WO2006037945A1, WO2006050803A1,WO2006090792A1, WO2006123088A2, WO2006125687A1, WO2006125688A1,WO2007003294A1, WO2007026834A1, WO2007071900A1, WO2007077201A1,WO2007077247A1, WO2007096576A1, WO2007119434A1, WO2007134984A1,WO2008009908A1, WO2008029084A1, WO2008059948A1, WO2008071918A1,WO2008074991A1, WO2008084073A1, WO2008100426A2, WO2008102908A1,WO2008152072A2, WO2008152073A2, WO2009000757A1, WO2009005297A2,WO2009035150A2, WO2009063180A1, WO2009068170A2, WO2009068171A2,WO2009086041A1, WO2009090401A2, WO2009090402A2, WO2009115788A1,WO2009116558A1, WO2009152995A1, WO2009158258A1, WO2010012649A1,WO2010012649A1, WO2010026989A1, WO2010034153A1, WO2010049270A1,WO2010049369A1, WO2010049405A1, WO2010049414A1, WO2010063422A1,WO2010069802A1, WO2010078906A2, WO2010078912A1, WO2010104217A1,WO2010108611A1, WO2010112826A3, WO2010116122A3, WO2010119906A1,WO2010130970A1, WO2011003776A2, WO2011035874A1, WO2011065451A1, all ofwhich are incorporated herein by reference.

An agronomic field in need of plant control is treated by application ofthe composition directly to the surface of the growing plants, such asby a spray. For example, the method is applied to control weeds in afield of crop plants by spraying the field with the composition. Thecomposition can be provided as a tank mix, a sequential treatment ofcomponents (generally the polynucleotide containing composition followedby the herbicide), or a simultaneous treatment or mixing of one or moreof the components of the composition from separate containers. Treatmentof the field can occur as often as needed to provide weed control andthe components of the composition can be adjusted to target specificweed species or weed families through utilization of specificpolynucleotides or polynucleotide compositions capable of selectivelytargeting the specific species or plant family to be controlled. Thecomposition can be applied at effective use rates according to the timeof application to the field, for example, preplant, at planting, postplanting, post harvest. PPG oxidase inhibitor herbicides can be appliedto a field at rates of 100 to 500 g ai/ha (active ingredient perhectare) or more. The polynucleotides of the composition can be appliedat rates of 1 to 30 grams per acre depending on the number of triggermolecules needed for the scope of weeds in the field.

Crop plants in which weed control is needed include, but are not limitedto, i) corn, soybean, cotton, canola, sugar beet, alfalfa, sugarcane,rice, and wheat; ii) vegetable plants including, but not limited to,tomato, sweet pepper, hot pepper, melon, watermelon, cucumber, eggplant,cauliflower, broccoli, lettuce, spinach, onion, peas, carrots, sweetcorn, Chinese cabbage, leek, fennel, pumpkin, squash or gourd, radish,Brussels sprouts, tomatillo, garden beans, dry beans, or okra; iii)culinary plants including, but not limited to, basil, parsley, coffee,or tea; iv) fruit plants including, but not limited to, apple, pear,cherry, peach, plum, apricot, banana, plantain, table grape, wine grape,citrus, avocado, mango, or berry; v) a tree grown for ornamental orcommercial use, including, but not limited to, a fruit or nut tree; orvi) an ornamental plant (e.g., an ornamental flowering plant or shrub orturf grass). The methods and compositions provided herein can also beapplied to plants produced by a cutting, cloning, or grafting process(i.e., a plant not grown from a seed) including fruit trees and plantsthat include, but are not limited to, citrus, apples, avocados,tomatoes, eggplant, cucumber, melons, watermelons, and grapes, as wellas various ornamental plants.

Pesticidal Mixtures

The polynucleotide compositions may also be used as mixtures withvarious agricultural chemicals and/or insecticides, miticides andfungicides, pesticidal and biopesticidal agents. Examples include butare not limited to azinphos-methyl, acephate, isoxathion, isofenphos,ethion, etrimfos, oxydemeton-methyl, oxydeprofos, quinalphos,chlorpyrifos, chlorpyrifos-methyl, chlorfenvinphos, cyanophos,dioxabenzofos, dichlorvos, disulfoton, dimethylvinphos, dimethoate,sulprofos, diazinon, thiometon, tetrachlorvinphos, temephos,tebupirimfos, terbufos, naled, vamidothion, pyraclofos, pyridafenthion,pirimiphos-methyl, fenitrothion, fenthion, phenthoate, flupyrazophos,prothiofos, propaphos, profenofos, phoxime, phosalone, phosmet,formothion, phorate, malathion, mecarbam, mesulfenfos, methamidophos,methidathion, parathion, methyl parathion, monocrotophos, trichlorphon,EPN, isazophos, isamidofos, cadusafos, diamidaphos, dichlofenthion,thionazin, fenamiphos, fosthiazate, fosthietan, phosphocarb, DSP,ethoprophos, alanycarb, aldicarb, isoprocarb, ethiofencarb, carbaryl,carbosulfan, xylylcarb, thiodicarb, pirimicarb, fenobucarb,furathiocarb, propoxur, bendiocarb, benfuracarb, methomyl, metolcarb,XMC, carbofuran, aldoxycarb, oxamyl, acrinathrin, allethrin,esfenvalerate, empenthrin, cycloprothrin, cyhalothrin,gamma-cyhalothrin, lambda-cyhalothrin, cyfluthrin, beta-cyfluthrin,cypermethrin, alpha-cypermethrin, zeta-cypermethrin, silafluofen,tetramethrin, tefluthrin, deltamethrin, tralomethrin, bifenthrin,phenothrin, fenvalerate, fenpropathrin, furamethrin, prallethrin,flucythrinate, fluvalinate, flubrocythrinate, permethrin, resmethrin,ethofenprox, cartap, thiocyclam, bensultap, acetamiprid, imidacloprid,clothianidin, dinotefuran, thiacloprid, thiamethoxam, nitenpyram,chlorfluazuron, diflubenzuron, teflubenzuron, triflumuron, novaluron,noviflumuron, bistrifluoron, fluazuron, flucycloxuron, flufenoxuron,hexaflumuron, lufenuron, chromafenozide, tebufenozide, halofenozide,methoxyfenozide, diofenolan, cyromazine, pyriproxyfen, buprofezin,methoprene, hydroprene, kinoprene, triazamate, endosulfan, chlorfenson,chlorobenzilate, dicofol, bromopropylate, acetoprole, fipronil,ethiprole, pyrethrin, rotenone, nicotine sulphate, BT (BacillusThuringiensis) agent, spinosad, abamectin, acequinocyl, amidoflumet,amitraz, etoxazole, chinomethionat, clofentezine, fenbutatin oxide,dienochlor, cyhexatin, spirodiclofen, spiromesifen, tetradifon,tebufenpyrad, binapacryl, bifenazate, pyridaben, pyrimidifen,fenazaquin, fenothiocarb, fenpyroximate, fluacrypyrim, fluazinam,flufenzin, hexythiazox, propargite, benzomate, polynactin complex,milbemectin, lufenuron, mecarbam, methiocarb, mevinphos, halfenprox,azadirachtin, diafenthiuron, indoxacarb, emamectin benzoate, potassiumoleate, sodium oleate, chlorfenapyr, tolfenpyrad, pymetrozine,fenoxycarb, hydramethylnon, hydroxy propyl starch, pyridalyl,flufenerim, flubendiamide, flonicamid, metaflumizole, lepimectin, TPIC,albendazole, oxibendazole, oxfendazole, trichlamide, fensulfothion,fenbendazole, levamisole hydrochloride, morantel tartrate, dazomet,metam-sodium, triadimefon, hexaconazole, propiconazole, ipconazole,prochloraz, triflumizole, tebuconazole, epoxiconazole, difenoconazole,flusilazole, triadimenol, cyproconazole, metconazole, fluquinconazole,bitertanol, tetraconazole, triticonazole, flutriafol, penconazole,diniconazole, fenbuconazole, bromuconazole, imibenconazole,simeconazole, myclobutanil, hymexazole, imazalil, furametpyr,thifluzamide, etridiazole, oxpoconazole, oxpoconazole fumarate,pefurazoate, prothioconazole, pyrifenox, fenarimol, nuarimol,bupirimate, mepanipyrim, cyprodinil, pyrimethanil, metalaxyl, mefenoxam,oxadixyl, benalaxyl, thiophanate, thiophanate-methyl, benomyl,carbendazim, fuberidazole, thiabendazole, manzeb, propineb, zineb,metiram, maneb, ziram, thiuram, chlorothalonil, ethaboxam, oxycarboxin,carboxin, flutolanil, silthiofam, mepronil, dimethomorph, fenpropidin,fenpropimorph, spiroxamine, tridemorph, dodemorph, flumorph,azoxystrobin, kresoxim-methyl, metominostrobin, orysastrobin,fluoxastrobin, trifloxystrobin, dimoxystrobin, pyraclostrobin,picoxystrobin, iprodione, procymidone, vinclozolin, chlozolinate,flusulfamide, dazomet, methyl isothiocyanate, chloropicrin,methasulfocarb, hydroxyisoxazole, potassium hydroxyisoxazole,echlomezol, D-D, carbam, basic copper chloride, basic copper sulfate,copper nonylphenolsulfonate, oxine copper, DBEDC, anhydrous coppersulfate, copper sulfate pentahydrate, cupric hydroxide, inorganicsulfur, wettable sulfur, lime sulfur, zinc sulfate, fentin, sodiumhydrogen carbonate, potassium hydrogen carbonate, sodium hypochlorite,silver, edifenphos, tolclofos-methyl, fosetyl, iprobenfos, dinocap,pyrazophos, carpropamid, fthalide, tricyclazole, pyroquilon, diclocymet,fenoxanil, kasugamycin, validamycin, polyoxins, blasticiden S,oxytetracycline, mildiomycin, streptomycin, rape seed oil, machine oil,benthiavalicarbisopropyl, iprovalicarb, propamocarb, diethofencarb,fluoroimide, fludioxanil, fenpiclonil, quinoxyfen, oxolinic acid,chlorothalonil, captan, folpet, probenazole, acibenzolar-S-methyl,tiadinil, cyflufenamid, fenhexamid, diflumetorim, metrafenone,picobenzamide, proquinazid, famoxadone, cyazofamid, fenamidone,zoxamide, boscalid, cymoxanil, dithianon, fluazinam, dichlofluanide,triforine, isoprothiolane, ferimzone, diclomezine, tecloftalam,pencycuron, chinomethionat, iminoctadine acetate, iminoctadinealbesilate, ambam, polycarbamate, thiadiazine, chloroneb, nickeldimethyldithiocarbamate, guazatine, dodecylguanidine-acetate,quintozene, tolylfluanid, anilazine, nitrothalisopropyl, fenitropan,dimethirimol, benthiazole, harpin protein, flumetover, mandipropamideand penthiopyrad.

Polynucleotides

As used herein, the terms “DNA,” “DNA molecule,” and “DNA polynucleotidemolecule” refer to a single-stranded DNA (ssDNA) or double-stranded DNA(dsDNA) molecule of genomic or synthetic origin, such as a polymer ofdeoxyribonucleotide bases or a DNA polynucleotide molecule. As usedherein, the terms “DNA sequence,” “DNA nucleotide sequence” or “DNApolynucleotide sequence” refer to the nucleotide sequence of a DNAmolecule. As used herein, the terms “RNA,” “RNA molecule,” “RNApolynucleotide molecule” refer to a single-stranded RNA (ssRNA) ordouble-stranded RNA (dsRNA) molecule of genomic or synthetic origin,such as a polymer of ribonucleotide bases that comprise single or doublestranded regions. Unless otherwise stated, nucleotide sequences in thetext of this specification are given, when read from left to right, inthe 5′ to 3′ direction. The nomenclature used herein is that required byTitle 37 of the United States Code of Federal Regulations §1.822 and setforth in the tables in WIPO Standard ST.25 (1998), Appendix 2, Tables 1and 3.

As used herein, “polynucleotide” refers to a DNA or RNA moleculecontaining multiple nucleotides and generally refers both to“oligonucleotides” (a polynucleotide molecule of typically 50 or fewernucleotides in length) and polynucleotides of 51 or more nucleotides.Embodiments include compositions including oligonucleotides having alength of 18-25 nucleotides (18-mers, 19-mers, 20-mers, 21-mers,22-mers, 23-mers, 24-mers, or 25-mers), for example, oligonucleotides ofTable 3 (SEQ ID NOs:1382-2221) or fragments thereof or medium-lengthpolynucleotides having a length of 26 or more nucleotides(polynucleotides of 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56,57, 58, 59, 60, about 65, about 70, about 75, about 80, about 85, about90, about 95, about 100, about 110, about 120, about 130, about 140,about 150, about 160, about 170, about 180, about 190, about 200, about210, about 220, about 230, about 240, about 250, about 260, about 270,about 280, about 290, or about 300 nucleotides), for example,oligonucleotides of Table 2 (SEQ ID NOs:72-1381) or fragments thereof orlong polynucleotides having a length greater than about 300 nucleotides(for example, polynucleotides of between about 300 to about 400nucleotides, between about 400 to about 500 nucleotides, between about500 to about 600 nucleotides, between about 600 to about 700nucleotides, between about 700 to about 800 nucleotides, between about800 to about 900 nucleotides, between about 900 to about 1000nucleotides, between about 300 to about 500 nucleotides, between about300 to about 600 nucleotides, between about 300 to about 700nucleotides, between about 300 to about 800 nucleotides, between about300 to about 900 nucleotides, or about 1000 nucleotides in length, oreven greater than about 1000 nucleotides in length, for example, up tothe entire length of a target gene including coding or non-coding orboth coding and non-coding portions of the target gene), for example,polynucleotides of Table 1 (SEQ ID NOs:1-71), wherein the selectedpolynucleotides or fragments thereof homologous or complementary to SEQID NOs:1-71 suppresses, represses or otherwise delay the expression ofthe target PPG oxidase gene. A target gene comprises any polynucleotidemolecule in a plant cell or fragment thereof for which the modulation ofthe expression of the target gene is provided by the methods andcompositions. Where a polynucleotide is double-stranded, its length canbe similarly described in terms of base pairs. Oligonucleotides andpolynucleotides can be made that are essentially identical oressentially complementary to adjacent genetic elements of a gene, forexample, spanning the junction region of an intron and exon, thejunction region of a promoter and a transcribed region, the junctionregion of a 5′ leader and a coding sequence, the junction of a 3′untranslated region and a coding sequence.

Polynucleotide compositions used in the various embodiments includecompositions including oligonucleotides or polynucleotides or a mixtureof both, including RNA or DNA or RNA/DNA hybrids or chemically modifiedoligonucleotides or polynucleotides or a mixture thereof. In someembodiments, the polynucleotide may be a combination of ribonucleotidesand deoxyribonucleotides, for example, synthetic polynucleotidesconsisting mainly of ribonucleotides but with one or more terminaldeoxyribonucleotides or synthetic polynucleotides consisting mainly ofdeoxyribonucleotides but with one or more terminaldideoxyribonucleotides. In some embodiments, the polynucleotide includesnon-canonical nucleotides such as inosine, thiouridine, orpseudouridine. In some embodiments, the polynucleotide includeschemically modified nucleotides. Examples of chemically modifiedoligonucleotides or polynucleotides are well known in the art; see, forexample, US Patent Publication 20110171287, US Patent Publication20110171176, US Patent Publication 20110152353, US Patent Publication,20110152346, and US Patent Publication 20110160082, each of which isherein incorporated by reference in its entirety. For example, includingbut not limited to the naturally occurring phosphodiester backbone of anoligonucleotide or polynucleotide can be partially or completelymodified with phosphorothioate, phosphorodithioate, or methylphosphonateinternucleotide linkage modifications, modified nucleoside bases ormodified sugars can be used in oligonucleotide or polynucleotidesynthesis, and oligonucleotides or polynucleotides can be labeled with afluorescent moiety (for example, fluorescein or rhodamine) or otherlabel (for example, biotin).

The polynucleotides can be single- or double-stranded RNA or single- ordouble-stranded DNA or double-stranded DNA/RNA hybrids or modifiedanalogues thereof, and can be of oligonucleotide lengths or longer. Inmore specific embodiments the polynucleotides that providesingle-stranded RNA in the plant cell are selected from the groupconsisting of (a) a single-stranded RNA molecule (ssRNA), (b) asingle-stranded RNA molecule that self-hybridizes to form adouble-stranded RNA molecule, (c) a double-stranded RNA molecule(dsRNA), (d) a single-stranded DNA molecule (ssDNA), (e) asingle-stranded DNA molecule that self-hybridizes to form adouble-stranded DNA molecule, (f) a single-stranded DNA moleculeincluding a modified Pol III gene that is transcribed to an RNAmolecule, (g) a double-stranded DNA molecule (dsDNA), (h) adouble-stranded DNA molecule including a modified Pol III gene that istranscribed to an RNA molecule, and (i) a double-stranded, hybridizedRNA/DNA molecule, or combinations thereof. In some embodiments, thesepolynucleotides include chemically modified nucleotides or non-canonicalnucleotides. In some embodiments, the oligonucleotides may beblunt-ended or may comprise a 3′ overhang of from 1-5 nucleotides of atleast one or both of the strands. Other configurations of theoligonucleotide are known in the field and are contemplated herein. Inembodiments of the method, the polynucleotides include double-strandedDNA formed by intramolecular hybridization, double-stranded DNA formedby intermolecular hybridization, double-stranded RNA formed byintramolecular hybridization, or double-stranded RNA formed byintermolecular hybridization. In one embodiment, the polynucleotidesinclude single-stranded DNA or single-stranded RNA that self-hybridizesto form a hairpin structure having an at least partially double-strandedstructure including at least one segment that will hybridize to RNAtranscribed from the gene targeted for suppression. Not intending to bebound by any mechanism, it is believed that such polynucleotides are orwill produce single-stranded RNA with at least one segment that willhybridize to RNA transcribed from the gene targeted for suppression. Incertain other embodiments, the polynucleotides further include apromoter, generally a promoter functional in a plant, for example, a polII promoter, a pol III promoter, a pol IV promoter, or a pol V promoter.

The term “gene” refers to components that comprise chromosomal DNA,plasmid DNA, cDNA, intron and exon DNA, artificial DNA polynucleotide,or other DNA that encodes a peptide, polypeptide, protein, or RNAtranscript molecule, and the genetic elements flanking the codingsequence that are involved in the regulation of expression, such aspromoter regions, 5′ leader regions, 3′ untranslated region that mayexist as native genes or transgenes in a plant genome. The gene or afragment thereof is isolated and subjected to polynucleotide sequencingmethods that determines the order of the nucleotides that comprise thegene. Any of the components of the gene are potential targets for atrigger oligonucleotide and polynucleotides.

The trigger polynucleotide molecules are designed to modulate expressionby inducing regulation or suppression of an endogenous PPG oxidase genein a plant and are designed to have a nucleotide sequence essentiallyidentical or essentially complementary to the nucleotide sequence of anendogenous PPG oxidase gene of a plant or to the sequence of RNAtranscribed from an endogenous PPG oxidase gene of a plant, including atransgene in a plant that provides for a herbicide resistant PPG oxidaseenzyme, which can be a coding sequence or a non-coding sequence.Effective molecules that modulate expression are referred to as “atrigger molecule, or trigger polynucleotides.” By “essentiallyidentical” or “essentially complementary” is meant that the triggerpolynucleotides (or at least one strand of a double-strandedpolynucleotide or portion thereof, or a portion of a single strandpolynucleotide) are designed to hybridize to the endogenous genenoncoding sequence or to RNA transcribed (known as messenger RNA or anRNA transcript) from the endogenous gene to effect regulation orsuppression of expression of the endogenous gene. Trigger molecules areidentified by “tiling” the gene targets with partially overlappingprobes or non-overlapping probes of antisense or sense polynucleotidesthat are essentially identical or essentially complementary to thenucleotide sequence of an endogenous gene. Multiple target sequences canbe aligned and sequence regions with homology in common, according tothe methods, are identified as potential trigger molecules for themultiple targets. Multiple trigger molecules of various lengths, forexample 18-25 nucleotides, 26-50 nucleotides, 51-100 nucleotides,101-200 nucleotides, 201-300 nucleotides or more can be pooled into afew treatments in order to investigate polynucleotide molecules thatcover a portion of a gene sequence (for example, a portion of a codingversus a portion of a noncoding region, or a 5′ versus a 3′ portion of agene) or an entire gene sequence including coding and noncoding regionsof a target gene. Polynucleotide molecules of the pooled triggermolecules can be divided into smaller pools or single molecules in orderto identify trigger molecules that provide the desired effect.

The target gene RNA and DNA polynucleotide molecules (Table 1, SEQ IDNOs:1-71) are sequenced by any number of available methods andequipment. Some of the sequencing technologies are availablecommercially, such as the sequencing-by-hybridization platform fromAffymetrix Inc. (Sunnyvale, Calif.) and the sequencing-by-synthesisplatforms from 454 Life Sciences (Bradford, Conn.), Illumina/Solexa(Hayward, Calif.) and Helicos Biosciences (Cambridge, Mass.), and thesequencing-by-ligation platform from Applied Biosystems (Foster City,Calif.), as described below. In addition to the single moleculesequencing performed using sequencing-by-synthesis of HelicosBiosciences, other single molecule sequencing technologies areencompassed by the method and include the SMRT™ technology of PacificBiosciences, the Ion Torrent™ technology, and nanopore sequencing beingdeveloped for example, by Oxford Nanopore Technologies. A PPG oxidasetarget gene comprising DNA or RNA can be isolated using primers orprobes essentially complementary or essentially homologous to SEQ IDNOs:1-71 or a fragment thereof. A polymerase chain reaction (PCR) genefragment can be produced using primers essentially complementary oressentially homologous to SEQ IDs NO:1-71 or a fragment thereof that isuseful to isolate a PPG oxidase gene from a plant genome. SEQ ID NOs:1-71 or fragments thereof can be used in various sequence capturetechnologies to isolate additional target gene sequences, for example,including but not limited to Roche NimbleGen® (Madison, Wis.) andStreptavdin-coupled Dynabeads® (Life Technologies, Grand Island, N.Y.)and US Patent Publication 20110015284, herein incorporated by referencein its entirety.

Embodiments of functional single-stranded polynucleotides have sequencecomplementarity that need not be 100 percent, but is at least sufficientto permit hybridization to RNA transcribed from the target gene or DNAof the target gene to form a duplex to permit a gene silencingmechanism. Thus, in embodiments, a polynucleotide fragment is designedto be essentially identical to, or essentially complementary to, asequence of 18 or more contiguous nucleotides in either the target PPGoxidase gene sequence or messenger RNA transcribed from the target gene.By “essentially identical” is meant having 100 percent sequence identityor at least about 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95,96, 97, 98, or 99 percent sequence identity when compared to thesequence of 18 or more contiguous nucleotides in either the target geneor RNA transcribed from the target gene; by “essentially complementary”is meant having 100 percent sequence complementarity or at least about83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99percent sequence complementarity when compared to the sequence of 18 ormore contiguous nucleotides in either the target gene or RNA transcribedfrom the target gene. In some embodiments, polynucleotide molecules aredesigned to have 100 percent sequence identity with or complementarityto one allele or one family member of a given target gene (coding ornon-coding sequence of a gene); in other embodiments, the polynucleotidemolecules are designed to have 100 percent sequence identity with orcomplementarity to multiple alleles or family members of a given targetgene. The trigger polynucleotide sequences in the sequence listing SEQID NOs: 1-2221 or table 1, 2 or 3 may be complementary or homologous toa portion of the PPG oxidase target gene sequence.

In certain embodiments, the polynucleotides used in the compositionsthat are essentially identical or essentially complementary to thetarget gene or transcript will comprise the predominant nucleic acid inthe composition. Thus in certain embodiments, the polynucleotides thatare essentially identical or essentially complementary to the targetgene or transcript will comprise at least about 50%, 75%, 95%, 98% or100% of the nucleic acids provided in the composition by either mass ormolar concentration. However, in certain embodiments, thepolynucleotides that are essentially identical or essentiallycomplementary to the target gene or transcript can comprise at leastabout 1% to about 50%, about 10% to about 50%, about 20% to about 50%,or about 30% to about 50% of the nucleic acids provided in thecomposition by either mass or molar concentration. Also provided arecompositions where the polynucleotides that are essentially identical oressentially complementary to the target gene or transcript can compriseat least about 1% to 100%, about 10% to 100%, about 20% to about 100%,about 30% to about 50%, or about 50% to a 100% of the nucleic acidsprovided in the composition by either mass or molar concentration.

“Identity” refers to the degree of similarity between two polynucleicacid or protein sequences. An alignment of the two sequences isperformed by a suitable computer program. A widely used and acceptedcomputer program for performing sequence alignments is CLUSTALW v1.6(Thompson et al., Nucl. Acids Res., 22: 4673-4680, 1994). The number ofmatching bases or amino acids is divided by the total number of bases oramino acids, and multiplied by 100 to obtain a percent identity. Forexample, if two 580 base pair sequences had 145 matched bases, theywould be 25 percent identical. If the two compared sequences are ofdifferent lengths, the number of matches is divided by the shorter ofthe two lengths. For example, if there are 100 matched amino acidsbetween a 200 and a 400 amino acid protein, they are 50 percentidentical with respect to the shorter sequence. If the shorter sequenceis less than 150 bases or 50 amino acids in length, the number ofmatches are divided by 150 (for nucleic acid bases) or 50 (for aminoacids), and multiplied by 100 to obtain a percent identity.

Trigger molecules for specific gene family members can be identifiedfrom coding and/or non-coding sequences of gene families of a plant ormultiple plants, by aligning and selecting 200-300 polynucleotidefragments from the least homologous regions amongst the alignedsequences and evaluated using topically applied polynucleotides (assense or anti-sense ssDNA or ssRNA, dsRNA or dsDNA) to determine theirrelative effectiveness in inducing the herbicidal phenotype. Theeffective segments are further subdivided into 50-60 polynucleotidefragments, prioritized by least homology, and reevaluated usingtopically applied polynucleotides. The effective 50-60 polynucleotidefragments are subdivided into 19-30 polynucleotide fragments,prioritized by least homology, and again evaluated for induction of theyield/quality phenotype. Once relative effectiveness is determined, thefragments are utilized singly, or again evaluated in combination withone or more other fragments to determine the trigger composition ormixture of trigger polynucleotides for providing the yield/qualityphenotype.

Trigger molecules for broad activity can be identified from codingand/or non-coding sequences of gene families of a plant or multipleplants, by aligning and selecting 200-300 polynucleotide fragments fromthe most homologous regions amongst the aligned sequences and evaluatedusing topically applied polynucleotides (as sense or anti-sense ssDNA orssRNA, dsRNA or dsDNA) to determine their relative effectiveness ininducing the yield/quality phenotype. The effective segments aresubdivided into 50-60 polynucleotide fragments, prioritized by mosthomology, and reevaluated using topically applied polynucleotides. Theeffective 50-60 polynucleotide fragments are subdivided into 19-30polynucleotide fragments, prioritized by most homology, and againevaluated for induction of the yield/quality phenotype. Once relativeeffectiveness is determined, the fragments may be utilized singly, or incombination with one or more other fragments to determine the triggercomposition or mixture of trigger polynucleotides for providing theyield/quality phenotype.

Methods of making polynucleotides are well known in the art. Chemicalsynthesis, in vivo synthesis and in vitro synthesis methods andcompositions are known in the art and include various viral elements,microbial cells, modified polymerases, and modified nucleotides.Commercial preparation of oligonucleotides often provides twodeoxyribonucleotides on the 3′ end of the sense strand. Longpolynucleotide molecules can be synthesized from commercially availablekits, for example, kits from Applied Biosystems/Ambion (Austin, Tex.)have DNA ligated on the 5′ end in a microbial expression cassette thatincludes a bacterial T7 polymerase promoter that makes RNA strands thatcan be assembled into a dsRNA and kits provided by various manufacturersthat include T7 RiboMax Express (Promega, Madison, Wis.), AmpliScribeT7-Flash (Epicentre, Madison, Wis.), and TranscriptAid T7 High Yield(Fermentas, Glen Burnie, Md.). dsRNA molecules can be produced frommicrobial expression cassettes in bacterial cells (Ongvarrasopone etal., ScienceAsia, 33:35-39; Yin, Appl. Microbiol. Biotechnol,84:323-333, 2009; Liu et al., BMC Biotechnology, 10:85, 2010) that haveregulated or deficient RNase III enzyme activity or the use of variousviral vectors to produce sufficient quantities of dsRNA. PPG oxidasegene fragments are inserted into the microbial expression cassettes in aposition in which the fragments are expressed to produce ssRNA or dsRNAuseful in the methods described herein to regulate expression on atarget PPG oxidase gene. Long polynucleotide molecules can also beassembled from multiple RNA or DNA fragments. In some embodiments designparameters such as Reynolds score (Reynolds et al., NatureBiotechnology, 22:326-330 (2004), Tuschl rules (Pei and Tuschl, NatureMethods, 3(9): 670-676, 2006), i-score (Nucleic Acids Res, 35:e123,2007), i-Score Designer tool and associated algorithms (Nucleic AcidsRes, 32:936-948, 2004; Biochem Biophys Res Commun, 316:1050-1058, 2004;Nucleic Acids Res, 32:893-901, 2004; Cell Cycle, 3: 790-5, 2004; NatBiotechnol, 23:995-1001, 2005; Nucleic Acids Res, 35:e27, 2007; BMCBioinformatics, 7:520, 2006; Nucleic Acids Res, 35:e123, 2007, NatBiotechnol, 22:326-330, 2004) are known in the art and may be used inselecting polynucleotide sequences effective in gene silencing. In someembodiments, the sequence of a polynucleotide is screened against thegenomic DNA of the intended plant to minimize unintentional silencing ofother genes.

The trigger polynucleotide and oligonucleotide molecule compositions areuseful in compositions, such as liquids that comprise thesepolynucleotide molecules, at low concentrations, alone or in combinationwith other components, for example one or more herbicide molecules,either in the same solution or in separately applied liquids that alsoprovide a transfer agent. While there is no upper limit on theconcentrations and dosages of polynucleotide molecules that can usefulin the methods, lower effective concentrations and dosages willgenerally be sought for efficiency. The concentrations can be adjustedin consideration of the volume of spray or treatment applied to plantleaves or other plant part surfaces, such as flower petals, stems,tubers, fruit, anthers, pollen, or seed. In one embodiment, a usefultreatment for herbaceous plants using 25-mer oligonucleotide moleculesis about 1 nanomole (nmol) of oligonucleotide molecules per plant, forexample, from about 0.05 to 1 nmol per plant. Other embodiments forherbaceous plants include useful ranges of about 0.05 to about 100 nmol,or about 0.1 to about 20 nmol, or about 1 nmol to about 10 nmol ofpolynucleotides per plant. Very large plants, trees, or vines mayrequire correspondingly larger amounts of polynucleotides. When usinglong dsRNA molecules that can be processed into multipleoligonucleotides, lower concentrations can be used. To illustratecertain embodiments, the factor 1×, when applied to oligonucleotidemolecules is arbitrarily used to denote a treatment of 0.8 nmol ofpolynucleotide molecule per plant; 10×, 8 nmol of polynucleotidemolecule per plant; and 100×, 80 nmol of polynucleotide molecule perplant.

The polynucleotide compositions are useful in compositions, such asliquids that comprise polynucleotide molecules, alone or in combinationwith other components either in the same liquid or in separately appliedliquids that provide a transfer agent. As used herein, a transfer agentis an agent that, when combined with a polynucleotide in a compositionthat is topically applied to a target plant surface, enables thepolynucleotide to enter a plant cell. In certain embodiments, a transferagent is an agent that conditions the surface of plant tissue, e.g.,leaves, stems, roots, flowers, or fruits, to permeation by thepolynucleotide molecules into plant cells. The transfer ofpolynucleotides into plant cells can be facilitated by the prior orcontemporaneous application of a polynucleotide-transferring agent tothe plant tissue. In some embodiments, the transferring agent is appliedsubsequent to the application of the polynucleotide composition. Thepolynucleotide transfer agent enables a pathway for polynucleotidesthrough cuticle wax barriers, stomata and/or cell wall or membranebarriers into plant cells. Suitable transfer agents to facilitatetransfer of the polynucleotide into a plant cell include agents thatincrease permeability of the exterior of the plant or that increasepermeability of plant cells to oligonucleotides or polynucleotides. Suchagents to facilitate transfer of the composition into a plant cellinclude a chemical agent, or a physical agent, or combinations thereof.Chemical agents for conditioning or transfer include (a) surfactants,(b) an organic solvent or an aqueous solution or aqueous mixtures oforganic solvents, (c) oxidizing agents, (d) acids, (e) bases, (f) oils,(g) enzymes, or combinations thereof. Embodiments of the method canoptionally include an incubation step, a neutralization step (e.g., toneutralize an acid, base, or oxidizing agent, or to inactivate anenzyme), a rinsing step, or combinations thereof. Embodiments of agentsor treatments for conditioning of a plant to permeation bypolynucleotides include emulsions, reverse emulsions, liposomes, andother micellar-like compositions. Embodiments of agents or treatmentsfor conditioning of a plant to permeation by polynucleotides includecounter-ions or other molecules that are known to associate with nucleicacid molecules, e.g., inorganic ammonium ions, alkyl ammonium ions,lithium ions, polyamines such as spermine, spermidine, or putrescine,and other cations. Organic solvents useful in conditioning a plant topermeation by polynucleotides include DMSO, DMF, pyridine,N-pyrrolidine, hexamethylphosphoramide, acetonitrile, dioxane,polypropylene glycol, other solvents miscible with water or that willdissolve phosphonucleotides in non-aqueous systems (such as is used insynthetic reactions). Naturally derived or synthetic oils with orwithout surfactants or emulsifiers can be used, e.g., plant-sourcedoils, crop oils (such as those listed in the 9^(th) Compendium ofHerbicide Adjuvants, publicly available on the worldwide web (internet)at herbicide.adjuvants.com) can be used, e.g., paraffinic oils, polyolfatty acid esters, or oils with short-chain molecules modified withamides or polyamines such as polyethyleneimine or N-pyrrolidine.Transfer agents include, but are not limited to, organosiliconepreparations.

Ligands can be tethered to a polynucleotide, for example, a dsRNA,ssRNA, dsDNA or ssDNA. Ligands in general can include modifiers, e.g.,for enhancing uptake; diagnostic compounds or reporter groups, e.g., formonitoring distribution; cross-linking agents; nuclease-resistanceconferring moieties; and natural or unusual nucleobases. Generalexamples include lipophiles, lipids (e.g., cholesterol), a bile acid, ora fatty acid (e.g., lithocholic-oleyl, lauroyl, docosnyl, stearoyl,palmitoyl, myristoyl oleoyl, linoleoyl), steroids (e.g., uvaol,hecigenin, diosgenin), terpenes (e.g., triterpenes, e.g.,sarsasapogenin, Friedelin, epifriedelanol derivatized lithocholic acid),vitamins (e.g., folic acid, vitamin A, biotin, pyridoxal),carbohydrates, proteins, protein binding agents, integrin targetingmolecules, polycationics, peptides, polyamines, and peptide mimics. Theligand may also be a recombinant or synthetic molecule, such as asynthetic polymer, e.g., polyethylene glycol (PEG), PEG-40K, PEG-20K andPEG-5K. Other examples of ligands include lipophilic molecules, e.g.,cholesterol, cholic acid, adamantane acetic acid, 1-pyrene butyric acid,dihydrotestosterone, glycerol (e.g., esters and ethers thereof, e.g.,C₁₀, C₁₁, C₁₂, C₁₃, C₁₄, C₁₅, C₁₆, C₁₇, C₁₈, C₁₉, or C₂₀ alkyl; e.g.,lauroyl, docosnyl, stearoyl, oleoyl, linoleoyl1,3-bis-O(hexadecyl)glycerol, 1,3-bis-O(octaadecyl)glycerol),geranyloxyhexyl group, hexadecylglycerol, borneol, menthol,1,3-propanediol, heptadecyl group, palmitic acid, myristic acid,O3-(oleoyl)lithocholic acid, O3-(oleoyl)cholenic acid, dodecanoyl,lithocholyl, 5.beta.-cholanyl, N,N-distearyl-lithocholamide,1,2-di-O-stearoylglyceride, dimethoxytrityl, or phenoxazine) and PEG(e.g., PEG-5K, PEG-20K, PEG-40K). Preferred lipophilic moieties includelipid, cholesterols, oleyl, retinyl, or cholesteryl residues.

Conjugating a ligand to a dsRNA can enhance its cellular absorption,lipophilic compounds that have been conjugated to oligonucleotidesinclude 1-pyrene butyric acid, 1,3-bis-O-(hexadecyl)glycerol, andmenthol. One example of a ligand for receptor-mediated endocytosis isfolic acid. Folic acid enters the cell by folate-receptor-radiatedendocytosis. dsRNA compounds bearing folic acid would be efficientlytransported into the cell via the folate-receptor-mediated endocytosis.Other ligands that have been conjugated to oligonucleotides includepolyethylene glycols, carbohydrate clusters, cross-linking agents,porphyrin conjugates, delivery peptides and lipids such as cholesterol.In certain instances, conjugation of a cationic ligand tooligonucleotides results in improved resistance to nucleases.Representative examples of cationic ligands are propylammonium anddimethylpropylammonium. Interestingly, antisense oligonucleotides werereported to retain their high binding affinity to mRNA when the cationicligand was dispersed, throughout the oligonucleotide. See Manoharan,Antisense Nucleic Acid Drug Dev., 12(2):103-128 (2002) and referencestherein.

A biologic delivery can be accomplished by a variety of methodsincluding, without limitation, (1) loading liposomes with a dsRNA acidmolecule provided herein and (2) complexing a dsRNA molecule with lipidsor liposomes to form nucleic acid-lipid or nucleic acid-liposomecomplexes. The liposome can be composed of cationic and neutral lipidscommonly used to transfect cells in vitro. Cationic lipids can complex(e.g., charge-associate) with negatively charged, nucleic acids to formliposomes. Examples of cationic liposomes include, without limitation,LIPOFECTIN® (Invitrogen/Life Technologies, Carlsbad, Calif., a 1:1 (w.w)liposome formulation of the cationic lipidN-[1-(2,3-dioleyloxy)propyl]-n,n,n-trimethylammonium chloride (DOTMA)and dioleoyl phophotidylethanolamine (DOPE)), LIPOFECTAMINE®*Invitrogen/Life Technologies, Carlsbad, Calif., a proprietary cationicliposome formulation), LIPOFECTACE® (Invitrogen/Life Technologies,Carlsbad, Calif., a liposome formulation of dimethyldioctadecyloammoniumbromide (DDAB) and DOPE), and DOTAP. Procedures for forming liposomesare well known in the art. Liposome compositions can be formed, forexample, from phosphatidylcholine, dimyristoyl phosphatidylcholine,dipalmitoyl phosphatidylcholine, dimyristoyl phosphatidyl glycerol,dioleoyl phosphatidylethanolamine or liposomes comprisingdihydrosphingomyelin (DHSM). Numerous lipophilic agents are commerciallyavailable, including LIPOFECTIN® (Invitrogen/Life Technologies,Carlsbad, Calif.) and EFFECTENE™ (Qiagen, Valencia, Calif., aproprietary non-liposomal lipid formulation). In addition, systemicdelivery methods can be optimized using commercially available cationiclipids such as DDAB or DOTAP, each of which can be mixed with a neutrallipid such as DOPE or cholesterol. In some cases, liposomes such asthose described by Templeton et al., Nature Biotechnology, 15:647-652(1997) can be used. In other embodiments, polycations such aspolyethyleneimine can be used to achieve delivery in vivo and ex vivo(Boletta et al., J. Am Soc. Nephrol., 7:1728, 1996). Additionalinformation regarding the use of liposomes to deliver nucleic acids canbe found in U.S. Pat. No. 6,271,359, PCT Publication WO 96/40964, andMorrissey, D. et al., 2005, Nature Biotechnol. 23(8):1002-7.

In certain embodiments, an organosilicone preparation that iscommercially available as Silwet® L-77 surfactant having CAS Number27306-78-1 and EPA Number: CAL.REG.NO. 5905-50073-AA, and currentlyavailable from Momentive Performance Materials, Albany, N.Y., can beused to prepare a polynucleotide composition. In certain embodimentswhere a Silwet L-77 organosilicone preparation is used as a pre-spraytreatment of plant leaves or other plant surfaces, freshly madeconcentrations in the range of about 0.015 to about 2 percent by weight(wt percent) (e.g., about 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04,0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095,0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4,1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.5 wt percent) areefficacious in preparing a leaf or other plant surface for transfer ofpolynucleotide molecules into plant cells from a topical application onthe surface. In certain embodiments of the methods and compositionsprovided herein, a composition that comprises a polynucleotide moleculeand an organosilicone preparation comprising Silwet L-77 in the range ofabout 0.015 to about 2 percent by weight (wt percent) (e.g., about 0.01,0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06, 0.065,0.07, 0.075, 0.08, 0.085, 0.09, 0.095, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6,0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0,2.1, 2.2, 2.3, 2.5 wt percent) is used or provided.

In certain embodiments, any of the commercially available organosiliconepreparations provided such as the following Breakthru S 321, Breakthru S200 Cat#67674-67-3, Breakthru OE 441 Cat#68937-55-3, Breakthru S 278 Cat#27306-78-1, Breakthru S 243, Breakthru S 233 Cat#134180-76-0, availablefrom manufacturer Evonik Goldschmidt (Germany), Silwet® HS 429, Silwet®HS 312, Silwet® HS 508, Silwet® HS 604 (Momentive Performance Materials,Albany, N.Y.) can be used as transfer agents in a polynucleotidecomposition. In certain embodiments where an organosilicone preparationis used as a pre-spray treatment of plant leaves or other surfaces,freshly made concentrations in the range of about 0.015 to about 2percent by weight (wt percent) (e.g., about 0.01, 0.015, 0.02, 0.025,0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08,0.085, 0.09, 0.095, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0,1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.5 wtpercent) are efficacious in preparing a leaf or other plant surface fortransfer of polynucleotide molecules into plant cells from a topicalapplication on the surface. In certain embodiments of the methods andcompositions provided herein, a composition that comprises apolynucleotide molecule and an organosilicone preparation in the rangeof about 0.015 to about 2 percent by weight (wt percent) (e. g., about0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06,0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095, 0.1, 0.2, 0.3, 0.4, 0.5,0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9,2.0, 2.1, 2.2, 2.3, 2.5 wt percent) is used or provided.

Organosilicone preparations used in the methods and compositionsprovided herein can comprise one or more effective organosiliconecompounds. As used herein, the phrase “effective organosiliconecompound” is used to describe any organosilicone compound that is foundin an organosilicone preparation that enables a polynucleotide to entera plant cell. In certain embodiments, an effective organosiliconecompound can enable a polynucleotide to enter a plant cell in a mannerpermitting a polynucleotide mediated suppression of a target geneexpression in the plant cell. In general, effective organosiliconecompounds include, but are not limited to, compounds that can comprise:i) a trisiloxane head group that is covalently linked to ii) an alkyllinker including, but not limited to, an n-propyl linker, that iscovalently linked to iii) a poly glycol chain, that is covalently linkedto iv) a terminal group. Trisiloxane head groups of such effectiveorganosilicone compounds include, but are not limited to,heptamethyltrisiloxane. Alkyl linkers can include, but are not limitedto, an n-propyl linker. Poly glycol chains include, but are not limitedto, polyethylene glycol or polypropylene glycol. Poly glycol chains cancomprise a mixture that provides an average chain length “n” of about“7.5.” In certain embodiments, the average chain length “n” can varyfrom about 5 to about 14. Terminal groups can include, but are notlimited to, alkyl groups such as a methyl group. Effectiveorganosilicone compounds are believed to include, but are not limitedto, trisiloxane ethoxylate surfactants or polyalkylene oxide modifiedheptamethyl trisiloxane.

In certain embodiments, an organosilicone preparation that comprises anorganosilicone compound comprising a trisiloxane head group is used inthe methods and compositions provided herein. In certain embodiments, anorganosilicone preparation that comprises an organosilicone compoundcomprising a heptamethyltrisiloxane head group is used in the methodsand compositions provided herein. In certain embodiments, anorganosilicone composition that comprises Compound I is used in themethods and compositions provided herein. In certain embodiments, anorganosilicone composition that comprises Compound I is used in themethods and compositions provided herein. In certain embodiments of themethods and compositions provided herein, a composition that comprises apolynucleotide molecule and one or more effective organosiliconecompounds in the range of about 0.015 to about 2 percent by weight (wtpercent) (e.g., about 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04,0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095,0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4,1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.5 wt percent) is used orprovided.

Compositions include but are not limited components that are one or morepolynucleotides essentially identical to, or essentially complementaryto, a PPG oxidase gene sequence (promoter, intron, exon, 5′ untranslatedregion, 3′ untranslated region), a transfer agent that provides for thepolynucleotide to enter a plant cell, a herbicide that complements theaction of the polynucleotide, one or more additional herbicides thatfurther enhance the herbicide activity of the composition or provide anadditional mode of action different from the complementing herbicide,various salts and stabilizing agents that enhance the utility of thecomposition as an admixture of the components of the composition.

The methods include one or more applications of a polynucleotidecomposition and one or more applications of a permeability-enhancingagent for conditioning of a plant to permeation by polynucleotides. Whenthe agent for conditioning to permeation is an organosiliconecomposition or compound contained therein, embodiments of thepolynucleotide molecules are double-stranded RNA oligonucleotides,single-stranded RNA oligonucleotides, double-stranded RNApolynucleotides, single-stranded RNA polynucleotides, double-strandedDNA oligonucleotides, single-stranded DNA oligonucleotides,double-stranded DNA polynucleotides, single-stranded DNApolynucleotides, chemically modified RNA or DNA oligonucleotides orpolynucleotides or mixtures thereof.

Compositions and methods are useful for modulating the expression of anendogenous PPG oxidase gene (for example, U.S. Pat. Nos. 7,838,263 and6,084,155) or transgenic PPG oxidase gene (U.S. Pat. Nos. 7,842,856 and7,485,777; US Patent Publ. 20070050863) in a plant cell. In variousembodiments, a PPG oxidase gene includes coding (protein-coding ortranslatable) sequence, non-coding (non-translatable) sequence, or bothcoding and non-coding sequence. Compositions can include polynucleotidesand oligonucleotides designed to target multiple genes, or multiplesegments of one or more genes. The target gene can include multipleconsecutive segments of a target gene, multiple non-consecutive segmentsof a target gene, multiple alleles of a target gene, or multiple targetgenes from one or more species.

A method is provided for modulating expression of a PPG oxidase gene ina plant including (a) conditioning of a plant to permeation bypolynucleotides and (b) treatment of the plant with the polynucleotidemolecules, wherein the polynucleotide molecules include at least onesegment of 18 or more contiguous nucleotides cloned from or otherwiseidentified from the target PPG oxidase gene in either anti-sense orsense orientation, whereby the polynucleotide molecules permeate theinterior of the plant and induce modulation of the target gene. Theconditioning and polynucleotide application can be performed separatelyor in a single step. When the conditioning and polynucleotideapplication are performed in separate steps, the conditioning canprecede or can follow the polynucleotide application within minutes,hours, or days. In some embodiments more than one conditioning step ormore than one polynucleotide molecule application can be performed onthe same plant. In embodiments of the method, the segment can be clonedor identified from (a) coding (protein-encoding), (b) non-coding(promoter and other gene related molecules), or (c) both coding andnon-coding parts of the target gene. Non-coding parts include DNA, suchas promoter regions or the RNA transcribed by the DNA that provide RNAregulatory molecules, including but not limited to: introns, 5′ or 3′untranslated regions, and microRNAs (miRNA), trans-acting siRNAs,natural anti-sense siRNAs, and other small RNAs with regulatory functionor RNAs having structural or enzymatic function including but notlimited to: ribozymes, ribosomal RNAs, t-RNAs, aptamers, andriboswitches.

All publications, patents and patent applications are hereinincorporated by reference to the same extent as if each individualpublication or patent application was specifically and individuallyindicated to be incorporated by reference.

The following examples are included to demonstrate examples of certainpreferred embodiments. It should be appreciated by those of skill in theart that the techniques disclosed in the examples that follow representapproaches the inventors have found function well in the practice of theinvention, and thus can be considered to constitute examples ofpreferred modes for its practice. However, those of skill in the artshould, in light of the present disclosure, appreciate that many changescan be made in the specific embodiments that are disclosed and stillobtain a like or similar result without departing from the spirit andscope of the invention.

EXAMPLES Example 1 Polynucleotides Related to the PPG Oxidase GeneSequences

The target PPG oxidase polynucleotide molecule naturally occurs in thegenome of Amaranthus albus, Amaranthus graecizans, Amaranthus hybridus,Amaranthus lividus, Amaranthus palmeri, Amaranthus rudis, Amaranthusspinosus, Amaranthus thunbergii, Amaranthus viridis, Ambrosia trifida,Chenopodium album, Commelina diffusa, Conyza canadensis, Digitariasanguinalis, Euphorbia heterophylla, Kochia scoparia, or Loliummultiflorum and include molecules related to the expression of apolypeptide identified as a PPG oxidase, that include regulatorymolecules, cDNAs comprising coding and noncoding regions of a PPGoxidase gene and fragments thereof as shown in Table 1.

Polynucleotide molecules were extracted from these plant species bymethods standard in the field, for example, total RNA was extractedusing Trizol Reagent (Invitrogen Corp, Carlsbad, Calif. Cat. No.15596-018), following the manufacturer's protocol or modificationsthereof by those skilled in the art of polynucleotide extraction thatmay enhance recovery or purity of the extracted RNA. Briefly, start with1 gram of ground plant tissue for extraction. Prealiquot 10 milliliters(mL) Trizol reagent to 15 mL conical tubes. Add ground powder to tubesand shake to homogenize. Incubate the homogenized samples for 5 minutes(min) at room temperature (RT) and then add 3 mL of chloroform. Shaketubes vigorously by hand for 15-30 seconds (sec) and incubate at RT for3 min. Centrifuge the tubes at 7,000 revolutions per minute (rpm) for 10min at 4 degrees C. Transfer the aqueous phase to a new 1.5 mL tube andadd 1 volume of cold isopropanol. Incubate the samples for 20-30 min atRT and centrifuge at 10,000 rpm for 10 min at 4 degrees C. Wash pelletwith Sigma-grade 80 percent ethanol. Remove the supernatant and brieflyair-dry the pellet. Dissolve the RNA pellet in approximately 200microliters of DEPC treated water. Heat briefly at 65 degrees C. todissolve pellet and vortex or pipet to resuspend RNA pellet. Adjust RNAconcentration to 1-2 microgram/microliter.

DNA is extracted using EZNA SP Plant DNA Mini kit (Omega Biotek,Norcross Ga., Cat#D5511) and Lysing Matrix E tubes (Q-Biogen, Cat#6914),following the manufacturer's protocol or modifications thereof by thoseskilled in the art of polynucleotide extraction that may enhancerecovery or purity of the extracted DNA. Briefly, aliquot ground tissueto a Lysing Matrix E tube on dry ice, add 800 μl Buffer SP1 to eachsample, homogenize in a bead beater for 35-45 sec, incubate on ice for45-60 sec, centrifuge at ≧14000 rpm for 1 min at RT, add 10 microliterRNase A to the lysate, incubate at 65° C. for 10 min, centrifuge for 1min at RT, add 280 μl Buffer SP2 and vortex to mix, incubate the sampleson ice for 5 min, centrifuge at ≧10,000 g for 10 min at RT, transfer thesupernatant to a homogenizer column in a 2 ml collection tube,centrifuge at 10,000 g for 2 min at RT, transfer the cleared lysate intoa 1.5 ml microfuge tube, add 1.5 volumes Buffer SP3 to the clearedlysate, vortex immediately to obtain a homogeneous mixture, transfer upto 650 μl supernatant to the Hi-Bind column, centrifuge at 10,000 g for1 min, repeat, apply 100 μl 65° C. Elution Buffer to the column,centrifuge at 10,000 g for 5 min at RT.

Next-generation DNA sequencers, such as the 454-FLX (Roche, Branford,Conn.), the SOLiD (Applied Biosystems), and the Genome Analyzer(HiSeq2000, Illumina, San Diego, Calif.) are used to providepolynucleotide sequences from the DNA and RNA extracted from the planttissues. Raw sequence data is assembled into contigs. The contigsequence is used to identify trigger molecules that can be applied tothe plant to enable regulation of the gene expression.

The target DNA sequence isolated from genomic (gDNA) and coding DNA(cDNA) from the various weedy plant species for the PPG oxidase gene andthe assembled contigs as set forth in SEQ ID Nos: 1-71 and Table 1below.

TABLE 1 SEQ ID NO SPECIES TYPE LENGTH 1 Amaranthus albus cDNA Contig1200 2 Amaranthus albus cDNA Contig 487 3 Amaranthus graecizans cDNAContig 1847 4 Amaranthus hybridus cDNA Contig 1170 5 Amaranthus lividuscDNA Contig 2344 6 Amaranthus palmeri cDNA Contig 1866 7 Amaranthuspalmeri cDNA Contig 1066 8 Amaranthus palmeri cDNA Contig 294 9Amaranthus palmeri gDNA Contig 6448 10 Amaranthus palmeri gDNA Contig5240 11 Amaranthus palmeri gDNA Contig 2936 12 Amaranthus palmeri gDNAContig 1923 13 Amaranthus palmeri gDNA Contig 2787 14 Amaranthus palmerigDNA Contig 2379 15 Amaranthus palmeri gDNA Contig 760 16 Amaranthusrudis cDNA Contig 1872 17 Amaranthus rudis cDNA Contig 1605 18Amaranthus rudis cDNA Contig 1238 19 Amaranthus rudis cDNA Contig 635 20Amaranthus rudis gDNA Contig 5704 21 Amaranthus rudis gDNA Contig 103122 Amaranthus rudis gDNA Contig 1017 23 Amaranthus rudis gDNA Contig 63024 Amaranthus rudis gDNA Contig 612 25 Amaranthus rudis gDNA Contig 48726 Amaranthus spinosus cDNA Contig 1582 27 Amaranthus thunbergii cDNAContig 1485 28 Amaranthus viridis cDNA Contig 1808 29 Ambrosia trifidacDNA Contig 1829 30 Ambrosia trifida cDNA Contig 921 31 Ambrosia trifidacDNA Contig 811 32 Ambrosia trifida gDNA Contig 4915 33 Ambrosia trifidagDNA Contig 851 34 Ambrosia trifida gDNA Contig 507 35 Chenopodium albumcDNA Contig 1648 36 Conyza canadensis cDNA Contig 1653 37 Conyzacanadensis cDNA Contig 600 38 Conyza canadensis gDNA Contig 10631 39Conyza canadensis gDNA Contig 10185 40 Conyza canadensis gDNA Contig2634 41 Euphorbia heterophylla cDNA Contig 1631 42 Euphorbiaheterophylla cDNA Contig 480 43 Euphorbia heterophylla gDNA Contig 406744 Euphorbia heterophylla gDNA Contig 2630 45 Euphorbia heterophyllagDNA Contig 1010 46 Euphorbia heterophylla gDNA Contig 855 47 Euphorbiaheterophylla gDNA Contig 121 48 Euphorbia heterophylla gDNA Contig 77 49Commelina diffusa cDNA Contig 444 50 Commelina diffusa gDNA Contig 304351 Commelina diffusa gDNA Contig 1559 52 Commelina diffusa gDNA Contig589 53 Digitaria sanguinalis cDNA Contig 876 54 Digitaria sanguinaliscDNA Contig 518 55 Digitaria sanguinalis cDNA Contig 387 56 Digitariasanguinalis gDNA Contig 2697 57 Digitaria sanguinalis gDNA Contig 108958 Kochia scoparia cDNA Contig 1518 59 Kochia scoparia cDNA Contig 96360 Kochia scoparia cDNA Contig 564 61 Kochia scoparia cDNA Contig 516 62Lolium multiflorum cDNA Contig 1185 63 Lolium multiflorum cDNA Contig258 64 Lolium multiflorum cDNA Contig 201 65 Lolium multiflorum cDNAContig 150 66 Lolium multiflorum cDNA Contig 144 67 Lolium multiflorumcDNA Contig 114 68 Lolium multiflorum cDNA Contig 108 69 Loliummultiflorum gDNA Contig 3037 70 Lolium multiflorum gDNA Contig 795 71Lolium multiflorum gDNA Contig 381

Example 2 Polynucleotides Related to the Trigger Molecules

The gene sequences and fragments of Table 1 were divided into 200polynucleotide (200-mer) lengths with 25 polynucleotide overlappingregions and are shown in Table 2, SEQ ID NOs:72-429. Thesepolynucleotides are tested to select the most efficacious triggerregions across the length of any target sequence. The triggerpolynucleotides are constructed as sense or anti-sense ssDNA or ssRNA,dsRNA or dsDNA, or dsDNA/RNA hybrids and combined with an organosiliconebased transfer agent to provide a polynucleotide preparation. Thepolynucleotides are combined into sets of two to three polynucleotidesper set, using 4-8 nmol of each polynucleotide. Each polynucleotide setis prepared with the transfer agent and applied to a plant or a field ofplants in combination with a PPG oxidase inhibitor containing herbicide,or followed by a PPG oxidase inhibitor treatment one to three days afterthe polynucleotide application, to determine the effect on the plant'ssusceptibility to a PPG oxidase inhibitor. The effect is measured asstunting the growth and/or killing of the plant and is measured 8-14days after treatment with the polynucleotide set and PPG oxidaseinhibitor. The most efficacious sets are identified and the individualpolynucleotides are tested in the same methods as the sets are and themost efficacious single 200-mer identified. The 200-mer sequence isdivided into smaller sequences of 50-70-mer regions with 10-15polynucleotide overlapping regions and the polynucleotides testedindividually. The most efficacious 50-70-mer is further divided intosmaller sequences of 25-mer regions with a 12 to 13 polynucleotideoverlapping region and tested for efficacy in combination with PPGoxidase inhibitor treatment. By this method, it is possible to identifyan oligonucleotide or several oligonucleotides that are the mostefficacious trigger molecule to effect plant sensitivity to a PPGoxidase inhibitor or modulation of a PPG oxidase gene expression. Themodulation of PPG oxidase gene expression is determined by the detectionof PPG oxidase siRNA molecules specific to a PPG oxidase gene or by anobservation of a reduction in the amount of PPG oxidase RNA transcriptproduced relative to an untreated plant or by merely observing theanticipated phenotype of the application of the trigger with the PPGoxidase inhibitor containing herbicide. Detection of siRNA can beaccomplished, for example, using kits such as mirVana (Ambion, AustinTex.) and mirPremier (Sigma-Aldrich, St Louis, Mo.).

The target DNA sequence isolated from genomic (gDNA) and coding DNA(cDNA) from the various weedy plant species for the PPG oxidase gene andthe assembled contigs as set forth in SEQ ID Nos: 1-71 were divided intopolynucleotide fragments as shown in Table 2 below and as set forth inSEQ ID Nos: 72-1381.

TABLE 2 SEQ Name | ID NO Species Reference Type Start End 72 Amaranthusalbus PPOX_B1_1 cDNA Contig 1 200 73 Amaranthus albus PPOX_B1_1 cDNAContig 1 200 74 Amaranthus albus PPOX_B1_2 cDNA Contig 176 375 75Amaranthus albus PPOX_B1_2 cDNA Contig 176 375 76 Amaranthus albusPPOX_B1_3 cDNA Contig 351 550 77 Amaranthus albus PPOX_B1_3 cDNA Contig351 550 78 Amaranthus albus PPOX_B1_4 cDNA Contig 526 725 79 Amaranthusalbus PPOX_B1_4 cDNA Contig 526 725 80 Amaranthus albus PPOX_B1_5 cDNAContig 701 900 81 Amaranthus albus PPOX_B1_5 cDNA Contig 701 900 82Amaranthus albus PPOX_B1_6 cDNA Contig 876 1075 83 Amaranthus albusPPOX_B1_6 cDNA Contig 876 1075 84 Amaranthus albus PPOX_B2_1 cDNA Contig1 200 85 Amaranthus albus PPOX_B2_1 cDNA Contig 1 200 86 Amaranthusalbus PPOX_B2_2 cDNA Contig 176 375 87 Amaranthus albus PPOX_B2_2 cDNAContig 176 375 88 Amaranthus graecizans PPOX_B3_1 cDNA Contig 1 200 89Amaranthus graecizans PPOX_B3_1 cDNA Contig 1 200 90 Amaranthusgraecizans PPOX_B3_2 cDNA Contig 176 375 91 Amaranthus graecizansPPOX_B3_2 cDNA Contig 176 375 92 Amaranthus graecizans PPOX_B3_3 cDNAContig 351 550 93 Amaranthus graecizans PPOX_B3_3 cDNA Contig 351 550 94Amaranthus graecizans PPOX_B3_4 cDNA Contig 526 725 95 Amaranthusgraecizans PPOX_B3_4 cDNA Contig 526 725 96 Amaranthus graecizansPPOX_B3_5 cDNA Contig 701 900 97 Amaranthus graecizans PPOX_B3_5 cDNAContig 701 900 98 Amaranthus graecizans PPOX_B3_6 cDNA Contig 876 107599 Amaranthus graecizans PPOX_B3_6 cDNA Contig 876 1075 100 Amaranthusgraecizans PPOX_B3_7 cDNA Contig 1051 1250 101 Amaranthus graecizansPPOX_B3_7 cDNA Contig 1051 1250 102 Amaranthus graecizans PPOX_B3_8 cDNAContig 1226 1425 103 Amaranthus graecizans PPOX_B3_8 cDNA Contig 12261425 104 Amaranthus graecizans PPOX_B3_9 cDNA Contig 1401 1600 105Amaranthus graecizans PPOX_B3_9 cDNA Contig 1401 1600 106 Amaranthusgraecizans PPOX_B3_10 cDNA Contig 1576 1775 107 Amaranthus graecizansPPOX_B3_10 cDNA Contig 1576 1775 108 Amaranthus hybridus PPOX_B4_1 cDNAContig 1 200 109 Amaranthus hybridus PPOX_B4_1 cDNA Contig 1 200 110Amaranthus hybridus PPOX_B4_2 cDNA Contig 176 375 111 Amaranthushybridus PPOX_B4_2 cDNA Contig 176 375 112 Amaranthus hybridus PPOX_B4_3cDNA Contig 351 550 113 Amaranthus hybridus PPOX_B4_3 cDNA Contig 351550 114 Amaranthus hybridus PPOX_B4_4 cDNA Contig 526 725 115 Amaranthushybridus PPOX_B4_4 cDNA Contig 526 725 116 Amaranthus hybridus PPOX_B4_5cDNA Contig 701 900 117 Amaranthus hybridus PPOX_B4_5 cDNA Contig 701900 118 Amaranthus hybridus PPOX_B4_6 cDNA Contig 876 1075 119Amaranthus hybridus PPOX_B4_6 cDNA Contig 876 1075 120 Amaranthuslividus PPOX_B5_1 cDNA Contig 1 200 121 Amaranthus lividus PPOX_B5_1cDNA Contig 1 200 122 Amaranthus lividus PPOX_B5_2 cDNA Contig 176 375123 Amaranthus lividus PPOX_B5_2 cDNA Contig 176 375 124 Amaranthuslividus PPOX_B5_3 cDNA Contig 351 550 125 Amaranthus lividus PPOX_B5_3cDNA Contig 351 550 126 Amaranthus lividus PPOX_B5_4 cDNA Contig 526 725127 Amaranthus lividus PPOX_B5_4 cDNA Contig 526 725 128 Amaranthuslividus PPOX_B5_5 cDNA Contig 701 900 129 Amaranthus lividus PPOX_B5_5cDNA Contig 701 900 130 Amaranthus lividus PPOX_B5_6 cDNA Contig 8761075 131 Amaranthus lividus PPOX_B5_6 cDNA Contig 876 1075 132Amaranthus lividus PPOX_B5_7 cDNA Contig 1051 1250 133 Amaranthuslividus PPOX_B5_7 cDNA Contig 1051 1250 134 Amaranthus lividus PPOX_B5_8cDNA Contig 1226 1425 135 Amaranthus lividus PPOX_B5_8 cDNA Contig 12261425 136 Amaranthus lividus PPOX_B5_9 cDNA Contig 1401 1600 137Amaranthus lividus PPOX_B5_9 cDNA Contig 1401 1600 138 Amaranthuslividus PPOX_B5_10 cDNA Contig 1576 1775 139 Amaranthus lividusPPOX_B5_10 cDNA Contig 1576 1775 140 Amaranthus lividus PPOX_B5_11 cDNAContig 1751 1950 141 Amaranthus lividus PPOX_B5_11 cDNA Contig 1751 1950142 Amaranthus lividus PPOX_B5_12 cDNA Contig 1926 2125 143 Amaranthuslividus PPOX_B5_12 cDNA Contig 1926 2125 144 Amaranthus lividusPPOX_B5_13 cDNA Contig 2101 2300 145 Amaranthus lividus PPOX_B5_13 cDNAContig 2101 2300 146 Amaranthus palmeri PPOX_B6_1 cDNA Contig 1 200 147Amaranthus palmeri PPOX_B6_1 cDNA Contig 1 200 148 Amaranthus palmeriPPOX_B6_2 cDNA Contig 176 375 149 Amaranthus palmeri PPOX_B6_2 cDNAContig 176 375 150 Amaranthus palmeri PPOX_B6_3 cDNA Contig 351 550 151Amaranthus palmeri PPOX_B6_3 cDNA Contig 351 550 152 Amaranthus palmeriPPOX_B6_4 cDNA Contig 526 725 153 Amaranthus palmeri PPOX_B6_4 cDNAContig 526 725 154 Amaranthus palmeri PPOX_B6_5 cDNA Contig 701 900 155Amaranthus palmeri PPOX_B6_5 cDNA Contig 701 900 156 Amaranthus palmeriPPOX_B6_6 cDNA Contig 876 1075 157 Amaranthus palmeri PPOX_B6_6 cDNAContig 876 1075 158 Amaranthus palmeri PPOX_B6_7 cDNA Contig 1051 1250159 Amaranthus palmeri PPOX_B6_7 cDNA Contig 1051 1250 160 Amaranthuspalmeri PPOX_B6_8 cDNA Contig 1226 1425 161 Amaranthus palmeri PPOX_B6_8cDNA Contig 1226 1425 162 Amaranthus palmeri PPOX_B6_9 cDNA Contig 14011600 163 Amaranthus palmeri PPOX_B6_9 cDNA Contig 1401 1600 164Amaranthus palmeri PPOX_B6_10 cDNA Contig 1576 1775 165 Amaranthuspalmeri PPOX_B6_10 cDNA Contig 1576 1775 166 Amaranthus palmeriPPOX_B13_1 gDNA Contig 1 200 167 Amaranthus palmeri PPOX_B13_1 gDNAContig 1 200 168 Amaranthus palmeri PPOX_B13_2 gDNA Contig 176 375 169Amaranthus palmeri PPOX_B13_2 gDNA Contig 176 375 170 Amaranthus palmeriPPOX_B13_3 gDNA Contig 351 550 171 Amaranthus palmeri PPOX_B13_3 gDNAContig 351 550 172 Amaranthus palmeri PPOX_B13_4 gDNA Contig 526 725 173Amaranthus palmeri PPOX_B13_4 gDNA Contig 526 725 174 Amaranthus palmeriPPOX_B13_5 gDNA Contig 701 900 175 Amaranthus palmeri PPOX_B13_5 gDNAContig 701 900 176 Amaranthus palmeri PPOX_B13_6 gDNA Contig 876 1075177 Amaranthus palmeri PPOX_B13_6 gDNA Contig 876 1075 178 Amaranthuspalmeri PPOX_B13_7 gDNA Contig 1051 1250 179 Amaranthus palmeriPPOX_B13_7 gDNA Contig 1051 1250 180 Amaranthus palmeri PPOX_B13_8 gDNAContig 1226 1425 181 Amaranthus palmeri PPOX_B13_8 gDNA Contig 1226 1425182 Amaranthus palmeri PPOX_B13_9 gDNA Contig 1401 1600 183 Amaranthuspalmeri PPOX_B13_9 gDNA Contig 1401 1600 184 Amaranthus palmeriPPOX_B13_10 gDNA Contig 1576 1775 185 Amaranthus palmeri PPOX_B13_10gDNA Contig 1576 1775 186 Amaranthus palmeri PPOX_B13_11 gDNA Contig1751 1950 187 Amaranthus palmeri PPOX_B13_11 gDNA Contig 1751 1950 188Amaranthus palmeri PPOX_B13_12 gDNA Contig 1926 2125 189 Amaranthuspalmeri PPOX_B13_12 gDNA Contig 1926 2125 190 Amaranthus palmeriPPOX_B13_13 gDNA Contig 2101 2300 191 Amaranthus palmeri PPOX_B13_13gDNA Contig 2101 2300 192 Amaranthus palmeri PPOX_B13_14 gDNA Contig2276 2475 193 Amaranthus palmeri PPOX_B13_14 gDNA Contig 2276 2475 194Amaranthus palmeri PPOX_B13_15 gDNA Contig 2451 2650 195 Amaranthuspalmeri PPOX_B13_15 gDNA Contig 2451 2650 196 Amaranthus palmeriPPOX_B10_1 gDNA Contig 1 200 197 Amaranthus palmeri PPOX_B10_1 gDNAContig 1 200 198 Amaranthus palmeri PPOX_B10_2 gDNA Contig 176 375 199Amaranthus palmeri PPOX_B10_2 gDNA Contig 176 375 200 Amaranthus palmeriPPOX_B10_3 gDNA Contig 351 550 201 Amaranthus palmeri PPOX_B10_3 gDNAContig 351 550 202 Amaranthus palmeri PPOX_B10_4 gDNA Contig 526 725 203Amaranthus palmeri PPOX_B10_4 gDNA Contig 526 725 204 Amaranthus palmeriPPOX_B10_5 gDNA Contig 701 900 205 Amaranthus palmeri PPOX_B10_5 gDNAContig 701 900 206 Amaranthus palmeri PPOX_B10_6 gDNA Contig 876 1075207 Amaranthus palmeri PPOX_B10_6 gDNA Contig 876 1075 208 Amaranthuspalmeri PPOX_B10_7 gDNA Contig 1051 1250 209 Amaranthus palmeriPPOX_B10_7 gDNA Contig 1051 1250 210 Amaranthus palmeri PPOX_B10_8 gDNAContig 1226 1425 211 Amaranthus palmeri PPOX_B10_8 gDNA Contig 1226 1425212 Amaranthus palmeri PPOX_B10_9 gDNA Contig 1401 1600 213 Amaranthuspalmeri PPOX_B10_9 gDNA Contig 1401 1600 214 Amaranthus palmeriPPOX_B10_10 gDNA Contig 1576 1775 215 Amaranthus palmeri PPOX_B10_10gDNA Contig 1576 1775 216 Amaranthus palmeri PPOX_B10_11 gDNA Contig1751 1950 217 Amaranthus palmeri PPOX_B10_11 gDNA Contig 1751 1950 218Amaranthus palmeri PPOX_B10_12 gDNA Contig 1926 2125 219 Amaranthuspalmeri PPOX_B10_12 gDNA Contig 1926 2125 220 Amaranthus palmeriPPOX_B10_13 gDNA Contig 2101 2300 221 Amaranthus palmeri PPOX_B10_13gDNA Contig 2101 2300 222 Amaranthus palmeri PPOX_B10_14 gDNA Contig2276 2475 223 Amaranthus palmeri PPOX_B10_14 gDNA Contig 2276 2475 224Amaranthus palmeri PPOX_B10_15 gDNA Contig 2451 2650 225 Amaranthuspalmeri PPOX_B10_15 gDNA Contig 2451 2650 226 Amaranthus palmeriPPOX_B10_16 gDNA Contig 2626 2825 227 Amaranthus palmeri PPOX_B10_16gDNA Contig 2626 2825 228 Amaranthus palmeri PPOX_B10_17 gDNA Contig2801 3000 229 Amaranthus palmeri PPOX_B10_17 gDNA Contig 2801 3000 230Amaranthus palmeri PPOX_B10_18 gDNA Contig 2976 3175 231 Amaranthuspalmeri PPOX_B10_18 gDNA Contig 2976 3175 232 Amaranthus palmeriPPOX_B10_19 gDNA Contig 3151 3350 233 Amaranthus palmeri PPOX_B10_19gDNA Contig 3151 3350 234 Amaranthus palmeri PPOX_B10_20 gDNA Contig3326 3525 235 Amaranthus palmeri PPOX_B10_20 gDNA Contig 3326 3525 236Amaranthus palmeri PPOX_B10_21 gDNA Contig 3501 3700 237 Amaranthuspalmeri PPOX_B10_21 gDNA Contig 3501 3700 238 Amaranthus palmeriPPOX_B10_22 gDNA Contig 3676 3875 239 Amaranthus palmeri PPOX_B10_22gDNA Contig 3676 3875 240 Amaranthus palmeri PPOX_B10_23 gDNA Contig3851 4050 241 Amaranthus palmeri PPOX_B10_23 gDNA Contig 3851 4050 242Amaranthus palmeri PPOX_B10_24 gDNA Contig 4026 4225 243 Amaranthuspalmeri PPOX_B10_24 gDNA Contig 4026 4225 244 Amaranthus palmeriPPOX_B10_25 gDNA Contig 4201 4400 245 Amaranthus palmeri PPOX_B10_25gDNA Contig 4201 4400 246 Amaranthus palmeri PPOX_B10_26 gDNA Contig4376 4575 247 Amaranthus palmeri PPOX_B10_26 gDNA Contig 4376 4575 248Amaranthus palmeri PPOX_B10_27 gDNA Contig 4551 4750 249 Amaranthuspalmeri PPOX_B10_27 gDNA Contig 4551 4750 250 Amaranthus palmeriPPOX_B10_28 gDNA Contig 4726 4925 251 Amaranthus palmeri PPOX_B10_28gDNA Contig 4726 4925 252 Amaranthus palmeri PPOX_B10_29 gDNA Contig4901 5100 253 Amaranthus palmeri PPOX_B10_29 gDNA Contig 4901 5100 254Amaranthus palmeri PPOX_B14_1 gDNA Contig 1 200 255 Amaranthus palmeriPPOX_B14_1 gDNA Contig 1 200 256 Amaranthus palmeri PPOX_B14_2 gDNAContig 176 375 257 Amaranthus palmeri PPOX_B14_2 gDNA Contig 176 375 258Amaranthus palmeri PPOX_B14_3 gDNA Contig 351 550 259 Amaranthus palmeriPPOX_B14_3 gDNA Contig 351 550 260 Amaranthus palmeri PPOX_B14_4 gDNAContig 526 725 261 Amaranthus palmeri PPOX_B14_4 gDNA Contig 526 725 262Amaranthus palmeri PPOX_B14_5 gDNA Contig 701 900 263 Amaranthus palmeriPPOX_B14_5 gDNA Contig 701 900 264 Amaranthus palmeri PPOX_B14_6 gDNAContig 876 1075 265 Amaranthus palmeri PPOX_B14_6 gDNA Contig 876 1075266 Amaranthus palmeri PPOX_B14_7 gDNA Contig 1051 1250 267 Amaranthuspalmeri PPOX_B14_7 gDNA Contig 1051 1250 268 Amaranthus palmeriPPOX_B14_8 gDNA Contig 1226 1425 269 Amaranthus palmeri PPOX_B14_8 gDNAContig 1226 1425 270 Amaranthus palmeri PPOX_B14_9 gDNA Contig 1401 1600271 Amaranthus palmeri PPOX_B14_9 gDNA Contig 1401 1600 272 Amaranthuspalmeri PPOX_B14_10 gDNA Contig 1576 1775 273 Amaranthus palmeriPPOX_B14_10 gDNA Contig 1576 1775 274 Amaranthus palmeri PPOX_B14_11gDNA Contig 1751 1950 275 Amaranthus palmeri PPOX_B14_11 gDNA Contig1751 1950 276 Amaranthus palmeri PPOX_B14_12 gDNA Contig 1926 2125 277Amaranthus palmeri PPOX_B14_12 gDNA Contig 1926 2125 278 Amaranthuspalmeri PPOX_B14_13 gDNA Contig 2101 2300 279 Amaranthus palmeriPPOX_B14_13 gDNA Contig 2101 2300 280 Amaranthus palmeri PPOX_B7_1 cDNAContig 1 200 281 Amaranthus palmeri PPOX_B7_1 cDNA Contig 1 200 282Amaranthus palmeri PPOX_B7_2 cDNA Contig 176 375 283 Amaranthus palmeriPPOX_B7_2 cDNA Contig 176 375 284 Amaranthus palmeri PPOX_B7_3 cDNAContig 351 550 285 Amaranthus palmeri PPOX_B7_3 cDNA Contig 351 550 286Amaranthus palmeri PPOX_B7_4 cDNA Contig 526 725 287 Amaranthus palmeriPPOX_B7_4 cDNA Contig 526 725 288 Amaranthus palmeri PPOX_B7_5 cDNAContig 701 900 289 Amaranthus palmeri PPOX_B7_5 cDNA Contig 701 900 290Amaranthus palmeri PPOX_B8_1 cDNA Contig 1 200 291 Amaranthus palmeriPPOX_B8_1 cDNA Contig 1 200 292 Amaranthus palmeri PPOX_B9_1 gDNA Contig1 200 293 Amaranthus palmeri PPOX_B9_1 gDNA Contig 1 200 294 Amaranthuspalmeri PPOX_B9_2 gDNA Contig 176 375 295 Amaranthus palmeri PPOX_B9_2gDNA Contig 176 375 296 Amaranthus palmeri PPOX_B9_3 gDNA Contig 351 550297 Amaranthus palmeri PPOX_B9_3 gDNA Contig 351 550 298 Amaranthuspalmeri PPOX_B9_4 gDNA Contig 526 725 299 Amaranthus palmeri PPOX_B9_4gDNA Contig 526 725 300 Amaranthus palmeri PPOX_B9_5 gDNA Contig 701 900301 Amaranthus palmeri PPOX_B9_5 gDNA Contig 701 900 302 Amaranthuspalmeri PPOX_B9_6 gDNA Contig 876 1075 303 Amaranthus palmeri PPOX_B9_6gDNA Contig 876 1075 304 Amaranthus palmeri PPOX_B9_7 gDNA Contig 10511250 305 Amaranthus palmeri PPOX_B9_7 gDNA Contig 1051 1250 306Amaranthus palmeri PPOX_B9_8 gDNA Contig 1226 1425 307 Amaranthuspalmeri PPOX_B9_8 gDNA Contig 1226 1425 308 Amaranthus palmeri PPOX_B9_9gDNA Contig 1401 1600 309 Amaranthus palmeri PPOX_B9_9 gDNA Contig 14011600 310 Amaranthus palmeri PPOX_B9_10 gDNA Contig 1576 1775 311Amaranthus palmeri PPOX_B9_10 gDNA Contig 1576 1775 312 Amaranthuspalmeri PPOX_B9_11 gDNA Contig 1751 1950 313 Amaranthus palmeriPPOX_B9_11 gDNA Contig 1751 1950 314 Amaranthus palmeri PPOX_B9_12 gDNAContig 1926 2125 315 Amaranthus palmeri PPOX_B9_12 gDNA Contig 1926 2125316 Amaranthus palmeri PPOX_B9_13 gDNA Contig 2101 2300 317 Amaranthuspalmeri PPOX_B9_13 gDNA Contig 2101 2300 318 Amaranthus palmeriPPOX_B9_14 gDNA Contig 2276 2475 319 Amaranthus palmeri PPOX_B9_14 gDNAContig 2276 2475 320 Amaranthus palmeri PPOX_B9_15 gDNA Contig 2451 2650321 Amaranthus palmeri PPOX_B9_15 gDNA Contig 2451 2650 322 Amaranthuspalmeri PPOX_B9_16 gDNA Contig 2626 2825 323 Amaranthus palmeriPPOX_B9_16 gDNA Contig 2626 2825 324 Amaranthus palmeri PPOX_B9_17 gDNAContig 2801 3000 325 Amaranthus palmeri PPOX_B9_17 gDNA Contig 2801 3000326 Amaranthus palmeri PPOX_B9_18 gDNA Contig 2976 3175 327 Amaranthuspalmeri PPOX_B9_18 gDNA Contig 2976 3175 328 Amaranthus palmeriPPOX_B9_19 gDNA Contig 3151 3350 329 Amaranthus palmeri PPOX_B9_19 gDNAContig 3151 3350 330 Amaranthus palmeri PPOX_B9_20 gDNA Contig 3326 3525331 Amaranthus palmeri PPOX_B9_20 gDNA Contig 3326 3525 332 Amaranthuspalmeri PPOX_B9_21 gDNA Contig 3501 3700 333 Amaranthus palmeriPPOX_B9_21 gDNA Contig 3501 3700 334 Amaranthus palmeri PPOX_B9_22 gDNAContig 3676 3875 335 Amaranthus palmeri PPOX_B9_22 gDNA Contig 3676 3875336 Amaranthus palmeri PPOX_B9_23 gDNA Contig 3851 4050 337 Amaranthuspalmeri PPOX_B9_23 gDNA Contig 3851 4050 338 Amaranthus palmeriPPOX_B9_24 gDNA Contig 4026 4225 339 Amaranthus palmeri PPOX_B9_24 gDNAContig 4026 4225 340 Amaranthus palmeri PPOX_B9_25 gDNA Contig 4201 4400341 Amaranthus palmeri PPOX_B9_25 gDNA Contig 4201 4400 342 Amaranthuspalmeri PPOX_B9_26 gDNA Contig 4376 4575 343 Amaranthus palmeriPPOX_B9_26 gDNA Contig 4376 4575 344 Amaranthus palmeri PPOX_B9_27 gDNAContig 4551 4750 345 Amaranthus palmeri PPOX_B9_27 gDNA Contig 4551 4750346 Amaranthus palmeri PPOX_B9_28 gDNA Contig 4726 4925 347 Amaranthuspalmeri PPOX_B9_28 gDNA Contig 4726 4925 348 Amaranthus palmeriPPOX_B9_29 gDNA Contig 4901 5100 349 Amaranthus palmeri PPOX_B9_29 gDNAContig 4901 5100 350 Amaranthus palmeri PPOX_B9_30 gDNA Contig 5076 5275351 Amaranthus palmeri PPOX_B9_30 gDNA Contig 5076 5275 352 Amaranthuspalmeri PPOX_B9_31 gDNA Contig 5251 5450 353 Amaranthus palmeriPPOX_B9_31 gDNA Contig 5251 5450 354 Amaranthus palmeri PPOX_B9_32 gDNAContig 5426 5625 355 Amaranthus palmeri PPOX_B9_32 gDNA Contig 5426 5625356 Amaranthus palmeri PPOX_B9_33 gDNA Contig 5601 5800 357 Amaranthuspalmeri PPOX_B9_33 gDNA Contig 5601 5800 358 Amaranthus palmeriPPOX_B9_34 gDNA Contig 5776 5975 359 Amaranthus palmeri PPOX_B9_34 gDNAContig 5776 5975 360 Amaranthus palmeri PPOX_B9_35 gDNA Contig 5951 6150361 Amaranthus palmeri PPOX_B9_35 gDNA Contig 5951 6150 362 Amaranthuspalmeri PPOX_B9_36 gDNA Contig 6126 6325 363 Amaranthus palmeriPPOX_B9_36 gDNA Contig 6126 6325 364 Amaranthus palmeri PPOX_B12_1 gDNAContig 1 200 365 Amaranthus palmeri PPOX_B12_1 gDNA Contig 1 200 366Amaranthus palmeri PPOX_B12_2 gDNA Contig 176 375 367 Amaranthus palmeriPPOX_B12_2 gDNA Contig 176 375 368 Amaranthus palmeri PPOX_B12_3 gDNAContig 351 550 369 Amaranthus palmeri PPOX_B12_3 gDNA Contig 351 550 370Amaranthus palmeri PPOX_B12_4 gDNA Contig 526 725 371 Amaranthus palmeriPPOX_B12_4 gDNA Contig 526 725 372 Amaranthus palmeri PPOX_B12_5 gDNAContig 701 900 373 Amaranthus palmeri PPOX_B12_5 gDNA Contig 701 900 374Amaranthus palmeri PPOX_B12_6 gDNA Contig 876 1075 375 Amaranthuspalmeri PPOX_B12_6 gDNA Contig 876 1075 376 Amaranthus palmeriPPOX_B12_7 gDNA Contig 1051 1250 377 Amaranthus palmeri PPOX_B12_7 gDNAContig 1051 1250 378 Amaranthus palmeri PPOX_B12_8 gDNA Contig 1226 1425379 Amaranthus palmeri PPOX_B12_8 gDNA Contig 1226 1425 380 Amaranthuspalmeri PPOX_B12_9 gDNA Contig 1401 1600 381 Amaranthus palmeriPPOX_B12_9 gDNA Contig 1401 1600 382 Amaranthus palmeri PPOX_B12_10 gDNAContig 1576 1775 383 Amaranthus palmeri PPOX_B12_10 gDNA Contig 15761775 384 Amaranthus palmeri PPOX_B15_1 gDNA Contig 1 200 385 Amaranthuspalmeri PPOX_B15_1 gDNA Contig 1 200 386 Amaranthus palmeri PPOX_B15_2gDNA Contig 176 375 387 Amaranthus palmeri PPOX_B15_2 gDNA Contig 176375 388 Amaranthus palmeri PPOX_B15_3 gDNA Contig 351 550 389 Amaranthuspalmeri PPOX_B15_3 gDNA Contig 351 550 390 Amaranthus palmeri PPOX_B15_4gDNA Contig 526 725 391 Amaranthus palmeri PPOX_B15_4 gDNA Contig 526725 392 Amaranthus palmeri PPOX_B11_1 gDNA Contig 1 200 393 Amaranthuspalmeri PPOX_B11_1 gDNA Contig 1 200 394 Amaranthus palmeri PPOX_B11_2gDNA Contig 176 375 395 Amaranthus palmeri PPOX_B11_2 gDNA Contig 176375 396 Amaranthus palmeri PPOX_B11_3 gDNA Contig 351 550 397 Amaranthuspalmeri PPOX_B11_3 gDNA Contig 351 550 398 Amaranthus palmeri PPOX_B11_4gDNA Contig 526 725 399 Amaranthus palmeri PPOX_B11_4 gDNA Contig 526725 400 Amaranthus palmeri PPOX_B11_5 gDNA Contig 701 900 401 Amaranthuspalmeri PPOX_B11_5 gDNA Contig 701 900 402 Amaranthus palmeri PPOX_B11_6gDNA Contig 876 1075 403 Amaranthus palmeri PPOX_B11_6 gDNA Contig 8761075 404 Amaranthus palmeri PPOX_B11_7 gDNA Contig 1051 1250 405Amaranthus palmeri PPOX_B11_7 gDNA Contig 1051 1250 406 Amaranthuspalmeri PPOX_B11_8 gDNA Contig 1226 1425 407 Amaranthus palmeriPPOX_B11_8 gDNA Contig 1226 1425 408 Amaranthus palmeri PPOX_B11_9 gDNAContig 1401 1600 409 Amaranthus palmeri PPOX_B11_9 gDNA Contig 1401 1600410 Amaranthus palmeri PPOX_B11_10 gDNA Contig 1576 1775 411 Amaranthuspalmeri PPOX_B11_10 gDNA Contig 1576 1775 412 Amaranthus palmeriPPOX_B11_11 gDNA Contig 1751 1950 413 Amaranthus palmeri PPOX_B11_11gDNA Contig 1751 1950 414 Amaranthus palmeri PPOX_B11_12 gDNA Contig1926 2125 415 Amaranthus palmeri PPOX_B11_12 gDNA Contig 1926 2125 416Amaranthus palmeri PPOX_B11_13 gDNA Contig 2101 2300 417 Amaranthuspalmeri PPOX_B11_13 gDNA Contig 2101 2300 418 Amaranthus palmeriPPOX_B11_14 gDNA Contig 2276 2475 419 Amaranthus palmeri PPOX_B11_14gDNA Contig 2276 2475 420 Amaranthus palmeri PPOX_B11_15 gDNA Contig2451 2650 421 Amaranthus palmeri PPOX_B11_15 gDNA Contig 2451 2650 422Amaranthus palmeri PPOX_B11_16 gDNA Contig 2626 2825 423 Amaranthuspalmeri PPOX_B11_16 gDNA Contig 2626 2825 424 Amaranthus rudisPPOX_B24_1 gDNA Contig 1 200 425 Amaranthus rudis PPOX_B24_1 gDNA Contig1 200 426 Amaranthus rudis PPOX_B24_2 gDNA Contig 176 375 427 Amaranthusrudis PPOX_B24_2 gDNA Contig 176 375 428 Amaranthus rudis PPOX_B24_3gDNA Contig 351 550 429 Amaranthus rudis PPOX_B24_3 gDNA Contig 351 550430 Amaranthus rudis PPOX_B22_1 gDNA Contig 1 200 431 Amaranthus rudisPPOX_B22_1 gDNA Contig 1 200 432 Amaranthus rudis PPOX_B22_2 gDNA Contig176 375 433 Amaranthus rudis PPOX_B22_2 gDNA Contig 176 375 434Amaranthus rudis PPOX_B22_3 gDNA Contig 351 550 435 Amaranthus rudisPPOX_B22_3 gDNA Contig 351 550 436 Amaranthus rudis PPOX_B22_4 gDNAContig 526 725 437 Amaranthus rudis PPOX_B22_4 gDNA Contig 526 725 438Amaranthus rudis PPOX_B22_5 gDNA Contig 701 900 439 Amaranthus rudisPPOX_B22_5 gDNA Contig 701 900 440 Amaranthus rudis PPOX_B17_1 cDNAContig 1 200 441 Amaranthus rudis PPOX_B17_1 cDNA Contig 1 200 442Amaranthus rudis PPOX_B17_2 cDNA Contig 176 375 443 Amaranthus rudisPPOX_B17_2 cDNA Contig 176 375 444 Amaranthus rudis PPOX_B17_3 cDNAContig 351 550 445 Amaranthus rudis PPOX_B17_3 cDNA Contig 351 550 446Amaranthus rudis PPOX_B17_4 cDNA Contig 526 725 447 Amaranthus rudisPPOX_B17_4 cDNA Contig 526 725 448 Amaranthus rudis PPOX_B17_5 cDNAContig 701 900 449 Amaranthus rudis PPOX_B17_5 cDNA Contig 701 900 450Amaranthus rudis PPOX_B17_6 cDNA Contig 876 1075 451 Amaranthus rudisPPOX_B17_6 cDNA Contig 876 1075 452 Amaranthus rudis PPOX_B17_7 cDNAContig 1051 1250 453 Amaranthus rudis PPOX_B17_7 cDNA Contig 1051 1250454 Amaranthus rudis PPOX_B17_8 cDNA Contig 1226 1425 455 Amaranthusrudis PPOX_B17_8 cDNA Contig 1226 1425 456 Amaranthus rudis PPOX_B17_9cDNA Contig 1401 1600 457 Amaranthus rudis PPOX_B17_9 cDNA Contig 14011600 458 Amaranthus rudis PPOX_B20_1 gDNA Contig 1 200 459 Amaranthusrudis PPOX_B20_1 gDNA Contig 1 200 460 Amaranthus rudis PPOX_B20_2 gDNAContig 176 375 461 Amaranthus rudis PPOX_B20_2 gDNA Contig 176 375 462Amaranthus rudis PPOX_B20_3 gDNA Contig 351 550 463 Amaranthus rudisPPOX_B20_3 gDNA Contig 351 550 464 Amaranthus rudis PPOX_B20_4 gDNAContig 526 725 465 Amaranthus rudis PPOX_B20_4 gDNA Contig 526 725 466Amaranthus rudis PPOX_B20_5 gDNA Contig 701 900 467 Amaranthus rudisPPOX_B20_5 gDNA Contig 701 900 468 Amaranthus rudis PPOX_B20_6 gDNAContig 876 1075 469 Amaranthus rudis PPOX_B20_6 gDNA Contig 876 1075 470Amaranthus rudis PPOX_B20_7 gDNA Contig 1051 1250 471 Amaranthus rudisPPOX_B20_7 gDNA Contig 1051 1250 472 Amaranthus rudis PPOX_B20_8 gDNAContig 1226 1425 473 Amaranthus rudis PPOX_B20_8 gDNA Contig 1226 1425474 Amaranthus rudis PPOX_B20_9 gDNA Contig 1401 1600 475 Amaranthusrudis PPOX_B20_9 gDNA Contig 1401 1600 476 Amaranthus rudis PPOX_B20_10gDNA Contig 1576 1775 477 Amaranthus rudis PPOX_B20_10 gDNA Contig 15761775 478 Amaranthus rudis PPOX_B20_11 gDNA Contig 1751 1950 479Amaranthus rudis PPOX_B20_11 gDNA Contig 1751 1950 480 Amaranthus rudisPPOX_B20_12 gDNA Contig 1926 2125 481 Amaranthus rudis PPOX_B20_12 gDNAContig 1926 2125 482 Amaranthus rudis PPOX_B20_13 gDNA Contig 2101 2300483 Amaranthus rudis PPOX_B20_13 gDNA Contig 2101 2300 484 Amaranthusrudis PPOX_B20_14 gDNA Contig 2276 2475 485 Amaranthus rudis PPOX_B20_14gDNA Contig 2276 2475 486 Amaranthus rudis PPOX_B20_15 gDNA Contig 24512650 487 Amaranthus rudis PPOX_B20_15 gDNA Contig 2451 2650 488Amaranthus rudis PPOX_B20_16 gDNA Contig 2626 2825 489 Amaranthus rudisPPOX_B20_16 gDNA Contig 2626 2825 490 Amaranthus rudis PPOX_B20_17 gDNAContig 2801 3000 491 Amaranthus rudis PPOX_B20_17 gDNA Contig 2801 3000492 Amaranthus rudis PPOX_B20_18 gDNA Contig 2976 3175 493 Amaranthusrudis PPOX_B20_18 gDNA Contig 2976 3175 494 Amaranthus rudis PPOX_B20_19gDNA Contig 3151 3350 495 Amaranthus rudis PPOX_B20_19 gDNA Contig 31513350 496 Amaranthus rudis PPOX_B20_20 gDNA Contig 3326 3525 497Amaranthus rudis PPOX_B20_20 gDNA Contig 3326 3525 498 Amaranthus rudisPPOX_B20_21 gDNA Contig 3501 3700 499 Amaranthus rudis PPOX_B20_21 gDNAContig 3501 3700 500 Amaranthus rudis PPOX_B20_22 gDNA Contig 3676 3875501 Amaranthus rudis PPOX_B20_22 gDNA Contig 3676 3875 502 Amaranthusrudis PPOX_B20_23 gDNA Contig 3851 4050 503 Amaranthus rudis PPOX_B20_23gDNA Contig 3851 4050 504 Amaranthus rudis PPOX_B20_24 gDNA Contig 40264225 505 Amaranthus rudis PPOX_B20_24 gDNA Contig 4026 4225 506Amaranthus rudis PPOX_B20_25 gDNA Contig 4201 4400 507 Amaranthus rudisPPOX_B20_25 gDNA Contig 4201 4400 508 Amaranthus rudis PPOX_B20_26 gDNAContig 4376 4575 509 Amaranthus rudis PPOX_B20_26 gDNA Contig 4376 4575510 Amaranthus rudis PPOX_B20_27 gDNA Contig 4551 4750 511 Amaranthusrudis PPOX_B20_27 gDNA Contig 4551 4750 512 Amaranthus rudis PPOX_B20_28gDNA Contig 4726 4925 513 Amaranthus rudis PPOX_B20_28 gDNA Contig 47264925 514 Amaranthus rudis PPOX_B20_29 gDNA Contig 4901 5100 515Amaranthus rudis PPOX_B20_29 gDNA Contig 4901 5100 516 Amaranthus rudisPPOX_B20_30 gDNA Contig 5076 5275 517 Amaranthus rudis PPOX_B20_30 gDNAContig 5076 5275 518 Amaranthus rudis PPOX_B20_31 gDNA Contig 5251 5450519 Amaranthus rudis PPOX_B20_31 gDNA Contig 5251 5450 520 Amaranthusrudis PPOX_B20_32 gDNA Contig 5426 5625 521 Amaranthus rudis PPOX_B20_32gDNA Contig 5426 5625 522 Amaranthus rudis PPOX_B18_1 cDNA Contig 1 200523 Amaranthus rudis PPOX_B18_1 cDNA Contig 1 200 524 Amaranthus rudisPPOX_B18_2 cDNA Contig 176 375 525 Amaranthus rudis PPOX_B18_2 cDNAContig 176 375 526 Amaranthus rudis PPOX_B18_3 cDNA Contig 351 550 527Amaranthus rudis PPOX_B18_3 cDNA Contig 351 550 528 Amaranthus rudisPPOX_B18_4 cDNA Contig 526 725 529 Amaranthus rudis PPOX_B18_4 cDNAContig 526 725 530 Amaranthus rudis PPOX_B18_5 cDNA Contig 701 900 531Amaranthus rudis PPOX_B18_5 cDNA Contig 701 900 532 Amaranthus rudisPPOX_B18_6 cDNA Contig 876 1075 533 Amaranthus rudis PPOX_B18_6 cDNAContig 876 1075 534 Amaranthus rudis PPOX_B16_1 cDNA Contig 1 200 535Amaranthus rudis PPOX_B16_1 cDNA Contig 1 200 536 Amaranthus rudisPPOX_B16_2 cDNA Contig 176 375 537 Amaranthus rudis PPOX_B16_2 cDNAContig 176 375 538 Amaranthus rudis PPOX_B16_3 cDNA Contig 351 550 539Amaranthus rudis PPOX_B16_3 cDNA Contig 351 550 540 Amaranthus rudisPPOX_B16_4 cDNA Contig 526 725 541 Amaranthus rudis PPOX_B16_4 cDNAContig 526 725 542 Amaranthus rudis PPOX_B16_5 cDNA Contig 701 900 543Amaranthus rudis PPOX_B16_5 cDNA Contig 701 900 544 Amaranthus rudisPPOX_B16_6 cDNA Contig 876 1075 545 Amaranthus rudis PPOX_B16_6 cDNAContig 876 1075 546 Amaranthus rudis PPOX_B16_7 cDNA Contig 1051 1250547 Amaranthus rudis PPOX_B16_7 cDNA Contig 1051 1250 548 Amaranthusrudis PPOX_B16_8 cDNA Contig 1226 1425 549 Amaranthus rudis PPOX_B16_8cDNA Contig 1226 1425 550 Amaranthus rudis PPOX_B16_9 cDNA Contig 14011600 551 Amaranthus rudis PPOX_B16_9 cDNA Contig 1401 1600 552Amaranthus rudis PPOX_B16_10 cDNA Contig 1576 1775 553 Amaranthus rudisPPOX_B16_10 cDNA Contig 1576 1775 554 Amaranthus rudis PPOX_B19_1 cDNAContig 1 200 555 Amaranthus rudis PPOX_B19_1 cDNA Contig 1 200 556Amaranthus rudis PPOX_B19_2 cDNA Contig 176 375 557 Amaranthus rudisPPOX_B19_2 cDNA Contig 176 375 558 Amaranthus rudis PPOX_B19_3 cDNAContig 351 550 559 Amaranthus rudis PPOX_B19_3 cDNA Contig 351 550 560Amaranthus rudis PPOX_B23_1 gDNA Contig 1 200 561 Amaranthus rudisPPOX_B23_1 gDNA Contig 1 200 562 Amaranthus rudis PPOX_B23_2 gDNA Contig351 550 563 Amaranthus rudis PPOX_B23_2 gDNA Contig 351 550 564Amaranthus rudis PPOX_B25_1 gDNA Contig 1 200 565 Amaranthus rudisPPOX_B25_1 gDNA Contig 1 200 566 Amaranthus rudis PPOX_B25_2 gDNA Contig176 375 567 Amaranthus rudis PPOX_B25_2 gDNA Contig 176 375 568Amaranthus rudis PPOX_B21_1 gDNA Contig 1 200 569 Amaranthus rudisPPOX_B21_1 gDNA Contig 1 200 570 Amaranthus rudis PPOX_B21_2 gDNA Contig176 375 571 Amaranthus rudis PPOX_B21_2 gDNA Contig 176 375 572Amaranthus rudis PPOX_B21_3 gDNA Contig 351 550 573 Amaranthus rudisPPOX_B21_3 gDNA Contig 351 550 574 Amaranthus rudis PPOX_B21_4 gDNAContig 526 725 575 Amaranthus rudis PPOX_B21_4 gDNA Contig 526 725 576Amaranthus rudis PPOX_B21_5 gDNA Contig 701 900 577 Amaranthus rudisPPOX_B21_5 gDNA Contig 701 900 578 Amaranthus spinosus PPOX_B26_1 cDNAContig 1 200 579 Amaranthus spinosus PPOX_B26_1 cDNA Contig 1 200 580Amaranthus spinosus PPOX_B26_2 cDNA Contig 176 375 581 Amaranthusspinosus PPOX_B26_2 cDNA Contig 176 375 582 Amaranthus spinosusPPOX_B26_3 cDNA Contig 351 550 583 Amaranthus spinosus PPOX_B26_3 cDNAContig 351 550 584 Amaranthus spinosus PPOX_B26_4 cDNA Contig 526 725585 Amaranthus spinosus PPOX_B26_4 cDNA Contig 526 725 586 Amaranthusspinosus PPOX_B26_5 cDNA Contig 701 900 587 Amaranthus spinosusPPOX_B26_5 cDNA Contig 701 900 588 Amaranthus spinosus PPOX_B26_6 cDNAContig 876 1075 589 Amaranthus spinosus PPOX_B26_6 cDNA Contig 876 1075590 Amaranthus spinosus PPOX_B26_7 cDNA Contig 1051 1250 591 Amaranthusspinosus PPOX_B26_7 cDNA Contig 1051 1250 592 Amaranthus spinosusPPOX_B26_8 cDNA Contig 1226 1425 593 Amaranthus spinosus PPOX_B26_8 cDNAContig 1226 1425 594 Amaranthus thunbergii PPOX_B27_1 cDNA Contig 1 200595 Amaranthus thunbergii PPOX_B27_1 cDNA Contig 1 200 596 Amaranthusthunbergii PPOX_B27_2 cDNA Contig 176 375 597 Amaranthus thunbergiiPPOX_B27_2 cDNA Contig 176 375 598 Amaranthus thunbergii PPOX_B27_3 cDNAContig 351 550 599 Amaranthus thunbergii PPOX_B27_3 cDNA Contig 351 550600 Amaranthus thunbergii PPOX_B27_4 cDNA Contig 526 725 601 Amaranthusthunbergii PPOX_B27_4 cDNA Contig 526 725 602 Amaranthus thunbergiiPPOX_B27_5 cDNA Contig 701 900 603 Amaranthus thunbergii PPOX_B27_5 cDNAContig 701 900 604 Amaranthus thunbergii PPOX_B27_6 cDNA Contig 876 1075605 Amaranthus thunbergii PPOX_B27_6 cDNA Contig 876 1075 606 Amaranthusthunbergii PPOX_B27_7 cDNA Contig 1051 1250 607 Amaranthus thunbergiiPPOX_B27_7 cDNA Contig 1051 1250 608 Amaranthus thunbergii PPOX_B27_8cDNA Contig 1226 1425 609 Amaranthus thunbergii PPOX_B27_8 cDNA Contig1226 1425 610 Amaranthus viridis PPOX_B28_1 cDNA Contig 1 200 611Amaranthus viridis PPOX_B28_1 cDNA Contig 1 200 612 Amaranthus viridisPPOX_B28_2 cDNA Contig 176 375 613 Amaranthus viridis PPOX_B28_2 cDNAContig 176 375 614 Amaranthus viridis PPOX_B28_3 cDNA Contig 351 550 615Amaranthus viridis PPOX_B28_3 cDNA Contig 351 550 616 Amaranthus viridisPPOX_B28_4 cDNA Contig 526 725 617 Amaranthus viridis PPOX_B28_4 cDNAContig 526 725 618 Amaranthus viridis PPOX_B28_5 cDNA Contig 701 900 619Amaranthus viridis PPOX_B28_5 cDNA Contig 701 900 620 Amaranthus viridisPPOX_B28_6 cDNA Contig 876 1075 621 Amaranthus viridis PPOX_B28_6 cDNAContig 876 1075 622 Amaranthus viridis PPOX_B28_7 cDNA Contig 1051 1250623 Amaranthus viridis PPOX_B28_7 cDNA Contig 1051 1250 624 Amaranthusviridis PPOX_B28_8 cDNA Contig 1226 1425 625 Amaranthus viridisPPOX_B28_8 cDNA Contig 1226 1425 626 Amaranthus viridis PPOX_B28_9 cDNAContig 1401 1600 627 Amaranthus viridis PPOX_B28_9 cDNA Contig 1401 1600628 Amaranthus viridis PPOX_B28_10 cDNA Contig 1576 1775 629 Amaranthusviridis PPOX_B28_10 cDNA Contig 1576 1775 630 Ambrosia trifidaPPOX_B34_1 gDNA Contig 1 200 631 Ambrosia trifida PPOX_B34_1 gDNA Contig1 200 632 Ambrosia trifida PPOX_B34_2 gDNA Contig 176 375 633 Ambrosiatrifida PPOX_B34_2 gDNA Contig 176 375 634 Ambrosia trifida PPOX_B32_1gDNA Contig 1 200 635 Ambrosia trifida PPOX_B32_1 gDNA Contig 1 200 636Ambrosia trifida PPOX_B32_2 gDNA Contig 176 375 637 Ambrosia trifidaPPOX_B32_2 gDNA Contig 176 375 638 Ambrosia trifida PPOX_B32_3 gDNAContig 351 550 639 Ambrosia trifida PPOX_B32_3 gDNA Contig 351 550 640Ambrosia trifida PPOX_B32_4 gDNA Contig 526 725 641 Ambrosia trifidaPPOX_B32_4 gDNA Contig 526 725 642 Ambrosia trifida PPOX_B32_5 gDNAContig 701 900 643 Ambrosia trifida PPOX_B32_5 gDNA Contig 701 900 644Ambrosia trifida PPOX_B32_6 gDNA Contig 876 1075 645 Ambrosia trifidaPPOX_B32_6 gDNA Contig 876 1075 646 Ambrosia trifida PPOX_B32_7 gDNAContig 1051 1250 647 Ambrosia trifida PPOX_B32_7 gDNA Contig 1051 1250648 Ambrosia trifida PPOX_B32_8 gDNA Contig 1226 1425 649 Ambrosiatrifida PPOX_B32_8 gDNA Contig 1226 1425 650 Ambrosia trifida PPOX_B32_9gDNA Contig 1401 1600 651 Ambrosia trifida PPOX_B32_9 gDNA Contig 14011600 652 Ambrosia trifida PPOX_B32_10 gDNA Contig 1576 1775 653 Ambrosiatrifida PPOX_B32_10 gDNA Contig 1576 1775 654 Ambrosia trifidaPPOX_B32_11 gDNA Contig 1751 1950 655 Ambrosia trifida PPOX_B32_11 gDNAContig 1751 1950 656 Ambrosia trifida PPOX_B32_12 gDNA Contig 1926 2125657 Ambrosia trifida PPOX_B32_12 gDNA Contig 1926 2125 658 Ambrosiatrifida PPOX_B32_13 gDNA Contig 2101 2300 659 Ambrosia trifidaPPOX_B32_13 gDNA Contig 2101 2300 660 Ambrosia trifida PPOX_B32_14 gDNAContig 2276 2475 661 Ambrosia trifida PPOX_B32_14 gDNA Contig 2276 2475662 Ambrosia trifida PPOX_B32_15 gDNA Contig 2451 2650 663 Ambrosiatrifida PPOX_B32_15 gDNA Contig 2451 2650 664 Ambrosia trifidaPPOX_B32_16 gDNA Contig 2626 2825 665 Ambrosia trifida PPOX_B32_16 gDNAContig 2626 2825 666 Ambrosia trifida PPOX_B32_17 gDNA Contig 2801 3000667 Ambrosia trifida PPOX_B32_17 gDNA Contig 2801 3000 668 Ambrosiatrifida PPOX_B32_18 gDNA Contig 2976 3175 669 Ambrosia trifidaPPOX_B32_18 gDNA Contig 2976 3175 670 Ambrosia trifida PPOX_B32_19 gDNAContig 3151 3350 671 Ambrosia trifida PPOX_B32_19 gDNA Contig 3151 3350672 Ambrosia trifida PPOX_B32_20 gDNA Contig 3326 3525 673 Ambrosiatrifida PPOX_B32_20 gDNA Contig 3326 3525 674 Ambrosia trifidaPPOX_B32_21 gDNA Contig 3501 3700 675 Ambrosia trifida PPOX_B32_21 gDNAContig 3501 3700 676 Ambrosia trifida PPOX_B32_22 gDNA Contig 3676 3875677 Ambrosia trifida PPOX_B32_22 gDNA Contig 3676 3875 678 Ambrosiatrifida PPOX_B32_23 gDNA Contig 3851 4050 679 Ambrosia trifidaPPOX_B32_23 gDNA Contig 3851 4050 680 Ambrosia trifida PPOX_B32_24 gDNAContig 4026 4225 681 Ambrosia trifida PPOX_B32_24 gDNA Contig 4026 4225682 Ambrosia trifida PPOX_B32_25 gDNA Contig 4201 4400 683 Ambrosiatrifida PPOX_B32_25 gDNA Contig 4201 4400 684 Ambrosia trifidaPPOX_B32_26 gDNA Contig 4376 4575 685 Ambrosia trifida PPOX_B32_26 gDNAContig 4376 4575 686 Ambrosia trifida PPOX_B32_27 gDNA Contig 4551 4750687 Ambrosia trifida PPOX_B32_27 gDNA Contig 4551 4750 688 Ambrosiatrifida PPOX_B30_1 cDNA Contig 1 200 689 Ambrosia trifida PPOX_B30_1cDNA Contig 1 200 690 Ambrosia trifida PPOX_B30_2 cDNA Contig 176 375691 Ambrosia trifida PPOX_B30_2 cDNA Contig 176 375 692 Ambrosia trifidaPPOX_B30_3 cDNA Contig 351 550 693 Ambrosia trifida PPOX_B30_3 cDNAContig 351 550 694 Ambrosia trifida PPOX_B30_4 cDNA Contig 526 725 695Ambrosia trifida PPOX_B30_4 cDNA Contig 526 725 696 Ambrosia trifidaPPOX_B30_5 cDNA Contig 701 900 697 Ambrosia trifida PPOX_B30_5 cDNAContig 701 900 698 Ambrosia trifida PPOX_B29_1 cDNA Contig 1 200 699Ambrosia trifida PPOX_B29_1 cDNA Contig 1 200 700 Ambrosia trifidaPPOX_B29_2 cDNA Contig 176 375 701 Ambrosia trifida PPOX_B29_2 cDNAContig 176 375 702 Ambrosia trifida PPOX_B29_3 cDNA Contig 351 550 703Ambrosia trifida PPOX_B29_3 cDNA Contig 351 550 704 Ambrosia trifidaPPOX_B29_4 cDNA Contig 526 725 705 Ambrosia trifida PPOX_B29_4 cDNAContig 526 725 706 Ambrosia trifida PPOX_B29_5 cDNA Contig 701 900 707Ambrosia trifida PPOX_B29_5 cDNA Contig 701 900 708 Ambrosia trifidaPPOX_B29_6 cDNA Contig 876 1075 709 Ambrosia trifida PPOX_B29_6 cDNAContig 876 1075 710 Ambrosia trifida PPOX_B29_7 cDNA Contig 1051 1250711 Ambrosia trifida PPOX_B29_7 cDNA Contig 1051 1250 712 Ambrosiatrifida PPOX_B29_8 cDNA Contig 1226 1425 713 Ambrosia trifida PPOX_B29_8cDNA Contig 1226 1425 714 Ambrosia trifida PPOX_B29_9 cDNA Contig 14011600 715 Ambrosia trifida PPOX_B29_9 cDNA Contig 1401 1600 716 Ambrosiatrifida PPOX_B29_10 cDNA Contig 1576 1775 717 Ambrosia trifidaPPOX_B29_10 cDNA Contig 1576 1775 718 Ambrosia trifida PPOX_B33_1 gDNAContig 1 200 719 Ambrosia trifida PPOX_B33_1 gDNA Contig 1 200 720Ambrosia trifida PPOX_B33_2 gDNA Contig 176 375 721 Ambrosia trifidaPPOX_B33_2 gDNA Contig 176 375 722 Ambrosia trifida PPOX_B33_3 gDNAContig 351 550 723 Ambrosia trifida PPOX_B33_3 gDNA Contig 351 550 724Ambrosia trifida PPOX_B33_4 gDNA Contig 526 725 725 Ambrosia trifidaPPOX_B33_4 gDNA Contig 526 725 726 Ambrosia trifida PPOX_B31_1 cDNAContig 1 200 727 Ambrosia trifida PPOX_B31_1 cDNA Contig 1 200 728Ambrosia trifida PPOX_B31_2 cDNA Contig 176 375 729 Ambrosia trifidaPPOX_B31_2 cDNA Contig 176 375 730 Ambrosia trifida PPOX_B31_3 cDNAContig 351 550 731 Ambrosia trifida PPOX_B31_3 cDNA Contig 351 550 732Ambrosia trifida PPOX_B31_4 cDNA Contig 526 725 733 Ambrosia trifidaPPOX_B31_4 cDNA Contig 526 725 734 Chenopodium album PPOX_B35_1 cDNAContig 1 200 735 Chenopodium album PPOX_B35_1 cDNA Contig 1 200 736Chenopodium album PPOX_B35_2 cDNA Contig 176 375 737 Chenopodium albumPPOX_B35_2 cDNA Contig 176 375 738 Chenopodium album PPOX_B35_3 cDNAContig 351 550 739 Chenopodium album PPOX_B35_3 cDNA Contig 351 550 740Chenopodium album PPOX_B35_4 cDNA Contig 526 725 741 Chenopodium albumPPOX_B35_4 cDNA Contig 526 725 742 Chenopodium album PPOX_B35_5 cDNAContig 701 900 743 Chenopodium album PPOX_B35_5 cDNA Contig 701 900 744Chenopodium album PPOX_B35_6 cDNA Contig 876 1075 745 Chenopodium albumPPOX_B35_6 cDNA Contig 876 1075 746 Chenopodium album PPOX_B35_7 cDNAContig 1051 1250 747 Chenopodium album PPOX_B35_7 cDNA Contig 1051 1250748 Chenopodium album PPOX_B35_8 cDNA Contig 1226 1425 749 Chenopodiumalbum PPOX_B35_8 cDNA Contig 1226 1425 750 Chenopodium album PPOX_B35_9cDNA Contig 1401 1600 751 Chenopodium album PPOX_B35_9 cDNA Contig 14011600 752 Commelina diffusa PPOX_B50_1 gDNA Contig 1 200 753 Commelinadiffusa PPOX_B50_1 gDNA Contig 1 200 754 Commelina diffusa PPOX_B50_2gDNA Contig 176 375 755 Commelina diffusa PPOX_B50_2 gDNA Contig 176 375756 Commelina diffusa PPOX_B50_3 gDNA Contig 351 550 757 Commelinadiffusa PPOX_B50_3 gDNA Contig 351 550 758 Commelina diffusa PPOX_B50_4gDNA Contig 526 725 759 Commelina diffusa PPOX_B50_4 gDNA Contig 526 725760 Commelina diffusa PPOX_B50_5 gDNA Contig 701 900 761 Commelinadiffusa PPOX_B50_5 gDNA Contig 701 900 762 Commelina diffusa PPOX_B50_6gDNA Contig 876 1075 763 Commelina diffusa PPOX_B50_6 gDNA Contig 8761075 764 Commelina diffusa PPOX_B50_7 gDNA Contig 1051 1250 765Commelina diffusa PPOX_B50_7 gDNA Contig 1051 1250 766 Commelina diffusaPPOX_B50_8 gDNA Contig 1226 1425 767 Commelina diffusa PPOX_B50_8 gDNAContig 1226 1425 768 Commelina diffusa PPOX_B50_9 gDNA Contig 1401 1600769 Commelina diffusa PPOX_B50_9 gDNA Contig 1401 1600 770 Commelinadiffusa PPOX_B50_10 gDNA Contig 1576 1775 771 Commelina diffusaPPOX_B50_10 gDNA Contig 1576 1775 772 Commelina diffusa PPOX_B50_11 gDNAContig 1751 1950 773 Commelina diffusa PPOX_B50_11 gDNA Contig 1751 1950774 Commelina diffusa PPOX_B50_12 gDNA Contig 1926 2125 775 Commelinadiffusa PPOX_B50_12 gDNA Contig 1926 2125 776 Commelina diffusaPPOX_B50_13 gDNA Contig 2101 2300 777 Commelina diffusa PPOX_B50_13 gDNAContig 2101 2300 778 Commelina diffusa PPOX_B50_14 gDNA Contig 2276 2475779 Commelina diffusa PPOX_B50_14 gDNA Contig 2276 2475 780 Commelinadiffusa PPOX_B50_15 gDNA Contig 2451 2650 781 Commelina diffusaPPOX_B50_15 gDNA Contig 2451 2650 782 Commelina diffusa PPOX_B50_16 gDNAContig 2626 2825 783 Commelina diffusa PPOX_B50_16 gDNA Contig 2626 2825784 Commelina diffusa PPOX_B50_17 gDNA Contig 2801 3000 785 Commelinadiffusa PPOX_B50_17 gDNA Contig 2801 3000 786 Commelina diffusaPPOX_B51_1 gDNA Contig 1 200 787 Commelina diffusa PPOX_B51_1 gDNAContig 1 200 788 Commelina diffusa PPOX_B51_2 gDNA Contig 176 375 789Commelina diffusa PPOX_B51_2 gDNA Contig 176 375 790 Commelina diffusaPPOX_B51_3 gDNA Contig 351 550 791 Commelina diffusa PPOX_B51_3 gDNAContig 351 550 792 Commelina diffusa PPOX_B51_4 gDNA Contig 526 725 793Commelina diffusa PPOX_B51_4 gDNA Contig 526 725 794 Commelina diffusaPPOX_B51_5 gDNA Contig 701 900 795 Commelina diffusa PPOX_B51_5 gDNAContig 701 900 796 Commelina diffusa PPOX_B51_6 gDNA Contig 876 1075 797Commelina diffusa PPOX_B51_6 gDNA Contig 876 1075 798 Commelina diffusaPPOX_B51_7 gDNA Contig 1051 1250 799 Commelina diffusa PPOX_B51_7 gDNAContig 1051 1250 800 Commelina diffusa PPOX_B51_8 gDNA Contig 1226 1425801 Commelina diffusa PPOX_B51_8 gDNA Contig 1226 1425 802 Commelinadiffusa PPOX_B49_1 cDNA Contig 1 200 803 Commelina diffusa PPOX_B49_1cDNA Contig 1 200 804 Commelina diffusa PPOX_B49_2 cDNA Contig 176 375805 Commelina diffusa PPOX_B49_2 cDNA Contig 176 375 806 Commelinadiffusa PPOX_B52_1 gDNA Contig 1 200 807 Commelina diffusa PPOX_B52_1gDNA Contig 1 200 808 Commelina diffusa PPOX_B52_2 gDNA Contig 176 375809 Commelina diffusa PPOX_B52_2 gDNA Contig 176 375 810 Commelinadiffusa PPOX_B52_3 gDNA Contig 351 550 811 Commelina diffusa PPOX_B52_3gDNA Contig 351 550 812 Conyza canadensis PPOX_B38_1 gDNA Contig 1 200813 Conyza canadensis PPOX_B38_1 gDNA Contig 1 200 814 Conyza canadensisPPOX_B38_2 gDNA Contig 176 375 815 Conyza canadensis PPOX_B38_2 gDNAContig 176 375 816 Conyza canadensis PPOX_B38_3 gDNA Contig 351 550 817Conyza canadensis PPOX_B38_3 gDNA Contig 351 550 818 Conyza canadensisPPOX_B38_4 gDNA Contig 526 725 819 Conyza canadensis PPOX_B38_4 gDNAContig 526 725 820 Conyza canadensis PPOX_B38_5 gDNA Contig 701 900 821Conyza canadensis PPOX_B38_5 gDNA Contig 701 900 822 Conyza canadensisPPOX_B38_6 gDNA Contig 876 1075 823 Conyza canadensis PPOX_B38_6 gDNAContig 876 1075 824 Conyza canadensis PPOX_B38_7 gDNA Contig 1051 1250825 Conyza canadensis PPOX_B38_7 gDNA Contig 1051 1250 826 Conyzacanadensis PPOX_B38_8 gDNA Contig 1226 1425 827 Conyza canadensisPPOX_B38_8 gDNA Contig 1226 1425 828 Conyza canadensis PPOX_B38_9 gDNAContig 1401 1600 829 Conyza canadensis PPOX_B38_9 gDNA Contig 1401 1600830 Conyza canadensis PPOX_B38_10 gDNA Contig 1576 1775 831 Conyzacanadensis PPOX_B38_10 gDNA Contig 1576 1775 832 Conyza canadensisPPOX_B38_11 gDNA Contig 1751 1950 833 Conyza canadensis PPOX_B38_11 gDNAContig 1751 1950 834 Conyza canadensis PPOX_B38_12 gDNA Contig 1926 2125835 Conyza canadensis PPOX_B38_12 gDNA Contig 1926 2125 836 Conyzacanadensis PPOX_B38_13 gDNA Contig 2101 2300 837 Conyza canadensisPPOX_B38_13 gDNA Contig 2101 2300 838 Conyza canadensis PPOX_B38_14 gDNAContig 2276 2475 839 Conyza canadensis PPOX_B38_14 gDNA Contig 2276 2475840 Conyza canadensis PPOX_B38_15 gDNA Contig 2451 2650 841 Conyzacanadensis PPOX_B38_15 gDNA Contig 2451 2650 842 Conyza canadensisPPOX_B38_16 gDNA Contig 2626 2825 843 Conyza canadensis PPOX_B38_16 gDNAContig 2626 2825 844 Conyza canadensis PPOX_B38_17 gDNA Contig 2801 3000845 Conyza canadensis PPOX_B38_17 gDNA Contig 2801 3000 846 Conyzacanadensis PPOX_B38_18 gDNA Contig 2976 3175 847 Conyza canadensisPPOX_B38_18 gDNA Contig 2976 3175 848 Conyza canadensis PPOX_B38_19 gDNAContig 3151 3350 849 Conyza canadensis PPOX_B38_19 gDNA Contig 3151 3350850 Conyza canadensis PPOX_B38_20 gDNA Contig 3326 3525 851 Conyzacanadensis PPOX_B38_20 gDNA Contig 3326 3525 852 Conyza canadensisPPOX_B38_21 gDNA Contig 3501 3700 853 Conyza canadensis PPOX_B38_21 gDNAContig 3501 3700 854 Conyza canadensis PPOX_B38_22 gDNA Contig 3676 3875855 Conyza canadensis PPOX_B38_22 gDNA Contig 3676 3875 856 Conyzacanadensis PPOX_B38_23 gDNA Contig 3851 4050 857 Conyza canadensisPPOX_B38_23 gDNA Contig 3851 4050 858 Conyza canadensis PPOX_B38_24 gDNAContig 4026 4225 859 Conyza canadensis PPOX_B38_24 gDNA Contig 4026 4225860 Conyza canadensis PPOX_B38_25 gDNA Contig 4201 4400 861 Conyzacanadensis PPOX_B38_25 gDNA Contig 4201 4400 862 Conyza canadensisPPOX_B38_26 gDNA Contig 4376 4575 863 Conyza canadensis PPOX_B38_26 gDNAContig 4376 4575 864 Conyza canadensis PPOX_B38_27 gDNA Contig 4551 4750865 Conyza canadensis PPOX_B38_27 gDNA Contig 4551 4750 866 Conyzacanadensis PPOX_B38_28 gDNA Contig 4726 4925 867 Conyza canadensisPPOX_B38_28 gDNA Contig 4726 4925 868 Conyza canadensis PPOX_B38_29 gDNAContig 4901 5100 869 Conyza canadensis PPOX_B38_29 gDNA Contig 4901 5100870 Conyza canadensis PPOX_B38_30 gDNA Contig 5076 5275 871 Conyzacanadensis PPOX_B38_30 gDNA Contig 5076 5275 872 Conyza canadensisPPOX_B38_31 gDNA Contig 5251 5450 873 Conyza canadensis PPOX_B38_31 gDNAContig 5251 5450 874 Conyza canadensis PPOX_B38_32 gDNA Contig 5426 5625875 Conyza canadensis PPOX_B38_32 gDNA Contig 5426 5625 876 Conyzacanadensis PPOX_B38_33 gDNA Contig 5601 5800 877 Conyza canadensisPPOX_B38_33 gDNA Contig 5601 5800 878 Conyza canadensis PPOX_B38_34 gDNAContig 5776 5975 879 Conyza canadensis PPOX_B38_34 gDNA Contig 5776 5975880 Conyza canadensis PPOX_B38_35 gDNA Contig 5951 6150 881 Conyzacanadensis PPOX_B38_35 gDNA Contig 5951 6150 882 Conyza canadensisPPOX_B38_36 gDNA Contig 6126 6325 883 Conyza canadensis PPOX_B38_36 gDNAContig 6126 6325 884 Conyza canadensis PPOX_B38_37 gDNA Contig 6301 6500885 Conyza canadensis PPOX_B38_37 gDNA Contig 6301 6500 886 Conyzacanadensis PPOX_B38_38 gDNA Contig 6476 6675 887 Conyza canadensisPPOX_B38_38 gDNA Contig 6476 6675 888 Conyza canadensis PPOX_B38_39 gDNAContig 6651 6850 889 Conyza canadensis PPOX_B38_39 gDNA Contig 6651 6850890 Conyza canadensis PPOX_B38_40 gDNA Contig 6826 7025 891 Conyzacanadensis PPOX_B38_40 gDNA Contig 6826 7025 892 Conyza canadensisPPOX_B38_41 gDNA Contig 7001 7200 893 Conyza canadensis PPOX_B38_41 gDNAContig 7001 7200 894 Conyza canadensis PPOX_B38_42 gDNA Contig 7176 7375895 Conyza canadensis PPOX_B38_42 gDNA Contig 7176 7375 896 Conyzacanadensis PPOX_B38_43 gDNA Contig 7351 7550 897 Conyza canadensisPPOX_B38_43 gDNA Contig 7351 7550 898 Conyza canadensis PPOX_B38_44 gDNAContig 7526 7725 899 Conyza canadensis PPOX_B38_44 gDNA Contig 7526 7725900 Conyza canadensis PPOX_B38_45 gDNA Contig 7701 7900 901 Conyzacanadensis PPOX_B38_45 gDNA Contig 7701 7900 902 Conyza canadensisPPOX_B38_46 gDNA Contig 7876 8075 903 Conyza canadensis PPOX_B38_46 gDNAContig 7876 8075 904 Conyza canadensis PPOX_B38_47 gDNA Contig 8051 8250905 Conyza canadensis PPOX_B38_47 gDNA Contig 8051 8250 906 Conyzacanadensis PPOX_B38_48 gDNA Contig 8226 8425 907 Conyza canadensisPPOX_B38_48 gDNA Contig 8226 8425 908 Conyza canadensis PPOX_B38_49 gDNAContig 8401 8600 909 Conyza canadensis PPOX_B38_49 gDNA Contig 8401 8600910 Conyza canadensis PPOX_B38_50 gDNA Contig 8576 8775 911 Conyzacanadensis PPOX_B38_50 gDNA Contig 8576 8775 912 Conyza canadensisPPOX_B38_51 gDNA Contig 8751 8950 913 Conyza canadensis PPOX_B38_51 gDNAContig 8751 8950 914 Conyza canadensis PPOX_B38_52 gDNA Contig 8926 9125915 Conyza canadensis PPOX_B38_52 gDNA Contig 8926 9125 916 Conyzacanadensis PPOX_B38_53 gDNA Contig 9101 9300 917 Conyza canadensisPPOX_B38_53 gDNA Contig 9101 9300 918 Conyza canadensis PPOX_B38_54 gDNAContig 9276 9475 919 Conyza canadensis PPOX_B38_54 gDNA Contig 9276 9475920 Conyza canadensis PPOX_B38_55 gDNA Contig 9451 9650 921 Conyzacanadensis PPOX_B38_55 gDNA Contig 9451 9650 922 Conyza canadensisPPOX_B38_56 gDNA Contig 9626 9825 923 Conyza canadensis PPOX_B38_56 gDNAContig 9626 9825 924 Conyza canadensis PPOX_B38_57 gDNA Contig 980110000 925 Conyza canadensis PPOX_B38_57 gDNA Contig 9801 10000 926Conyza canadensis PPOX_B38_58 gDNA Contig 9976 10175 927 Conyzacanadensis PPOX_B38_58 gDNA Contig 9976 10175 928 Conyza canadensisPPOX_B38_59 gDNA Contig 10151 10350 929 Conyza canadensis PPOX_B38_59gDNA Contig 10151 10350 930 Conyza canadensis PPOX_B38_60 gDNA Contig10326 10525 931 Conyza canadensis PPOX_B38_60 gDNA Contig 10326 10525932 Conyza canadensis PPOX_B39_1 gDNA Contig 1 200 933 Conyza canadensisPPOX_B39_1 gDNA Contig 1 200 934 Conyza canadensis PPOX_B39_2 gDNAContig 176 375 935 Conyza canadensis PPOX_B39_2 gDNA Contig 176 375 936Conyza canadensis PPOX_B39_3 gDNA Contig 351 550 937 Conyza canadensisPPOX_B39_3 gDNA Contig 351 550 938 Conyza canadensis PPOX_B39_4 gDNAContig 526 725 939 Conyza canadensis PPOX_B39_4 gDNA Contig 526 725 940Conyza canadensis PPOX_B39_5 gDNA Contig 701 900 941 Conyza canadensisPPOX_B39_5 gDNA Contig 701 900 942 Conyza canadensis PPOX_B39_6 gDNAContig 876 1075 943 Conyza canadensis PPOX_B39_6 gDNA Contig 876 1075944 Conyza canadensis PPOX_B39_7 gDNA Contig 1051 1250 945 Conyzacanadensis PPOX_B39_7 gDNA Contig 1051 1250 946 Conyza canadensisPPOX_B39_8 gDNA Contig 1226 1425 947 Conyza canadensis PPOX_B39_8 gDNAContig 1226 1425 948 Conyza canadensis PPOX_B39_9 gDNA Contig 1401 1600949 Conyza canadensis PPOX_B39_9 gDNA Contig 1401 1600 950 Conyzacanadensis PPOX_B39_10 gDNA Contig 1576 1775 951 Conyza canadensisPPOX_B39_10 gDNA Contig 1576 1775 952 Conyza canadensis PPOX_B39_11 gDNAContig 1751 1950 953 Conyza canadensis PPOX_B39_11 gDNA Contig 1751 1950954 Conyza canadensis PPOX_B39_12 gDNA Contig 1926 2125 955 Conyzacanadensis PPOX_B39_12 gDNA Contig 1926 2125 956 Conyza canadensisPPOX_B39_13 gDNA Contig 2101 2300 957 Conyza canadensis PPOX_B39_13 gDNAContig 2101 2300 958 Conyza canadensis PPOX_B39_14 gDNA Contig 2276 2475959 Conyza canadensis PPOX_B39_14 gDNA Contig 2276 2475 960 Conyzacanadensis PPOX_B39_15 gDNA Contig 2451 2650 961 Conyza canadensisPPOX_B39_15 gDNA Contig 2451 2650 962 Conyza canadensis PPOX_B39_16 gDNAContig 2626 2825 963 Conyza canadensis PPOX_B39_16 gDNA Contig 2626 2825964 Conyza canadensis PPOX_B39_17 gDNA Contig 2801 3000 965 Conyzacanadensis PPOX_B39_17 gDNA Contig 2801 3000 966 Conyza canadensisPPOX_B39_18 gDNA Contig 2976 3175 967 Conyza canadensis PPOX_B39_18 gDNAContig 2976 3175 968 Conyza canadensis PPOX_B39_19 gDNA Contig 3151 3350969 Conyza canadensis PPOX_B39_19 gDNA Contig 3151 3350 970 Conyzacanadensis PPOX_B39_20 gDNA Contig 3326 3525 971 Conyza canadensisPPOX_B39_20 gDNA Contig 3326 3525 972 Conyza canadensis PPOX_B39_21 gDNAContig 3501 3700 973 Conyza canadensis PPOX_B39_21 gDNA Contig 3501 3700974 Conyza canadensis PPOX_B39_22 gDNA Contig 3676 3875 975 Conyzacanadensis PPOX_B39_22 gDNA Contig 3676 3875 976 Conyza canadensisPPOX_B39_23 gDNA Contig 3851 4050 977 Conyza canadensis PPOX_B39_23 gDNAContig 3851 4050 978 Conyza canadensis PPOX_B39_24 gDNA Contig 4026 4225979 Conyza canadensis PPOX_B39_24 gDNA Contig 4026 4225 980 Conyzacanadensis PPOX_B39_25 gDNA Contig 4201 4400 981 Conyza canadensisPPOX_B39_25 gDNA Contig 4201 4400 982 Conyza canadensis PPOX_B39_26 gDNAContig 4376 4575 983 Conyza canadensis PPOX_B39_26 gDNA Contig 4376 4575984 Conyza canadensis PPOX_B39_27 gDNA Contig 4551 4750 985 Conyzacanadensis PPOX_B39_27 gDNA Contig 4551 4750 986 Conyza canadensisPPOX_B39_28 gDNA Contig 4726 4925 987 Conyza canadensis PPOX_B39_28 gDNAContig 4726 4925 988 Conyza canadensis PPOX_B39_29 gDNA Contig 4901 5100989 Conyza canadensis PPOX_B39_29 gDNA Contig 4901 5100 990 Conyzacanadensis PPOX_B39_30 gDNA Contig 5076 5275 991 Conyza canadensisPPOX_B39_30 gDNA Contig 5076 5275 992 Conyza canadensis PPOX_B39_31 gDNAContig 5251 5450 993 Conyza canadensis PPOX_B39_31 gDNA Contig 5251 5450994 Conyza canadensis PPOX_B39_32 gDNA Contig 5426 5625 995 Conyzacanadensis PPOX_B39_32 gDNA Contig 5426 5625 996 Conyza canadensisPPOX_B39_33 gDNA Contig 5601 5800 997 Conyza canadensis PPOX_B39_33 gDNAContig 5601 5800 998 Conyza canadensis PPOX_B39_34 gDNA Contig 5776 5975999 Conyza canadensis PPOX_B39_34 gDNA Contig 5776 5975 1000 Conyzacanadensis PPOX_B39_35 gDNA Contig 5951 6150 1001 Conyza canadensisPPOX_B39_35 gDNA Contig 5951 6150 1002 Conyza canadensis PPOX_B39_36gDNA Contig 6126 6325 1003 Conyza canadensis PPOX_B39_36 gDNA Contig6126 6325 1004 Conyza canadensis PPOX_B39_37 gDNA Contig 6301 6500 1005Conyza canadensis PPOX_B39_37 gDNA Contig 6301 6500 1006 Conyzacanadensis PPOX_B39_38 gDNA Contig 6476 6675 1007 Conyza canadensisPPOX_B39_38 gDNA Contig 6476 6675 1008 Conyza canadensis PPOX_B39_39gDNA Contig 6651 6850 1009 Conyza canadensis PPOX_B39_39 gDNA Contig6651 6850 1010 Conyza canadensis PPOX_B39_40 gDNA Contig 6826 7025 1011Conyza canadensis PPOX_B39_40 gDNA Contig 6826 7025 1012 Conyzacanadensis PPOX_B39_41 gDNA Contig 7001 7200 1013 Conyza canadensisPPOX_B39_41 gDNA Contig 7001 7200 1014 Conyza canadensis PPOX_B39_42gDNA Contig 7176 7375 1015 Conyza canadensis PPOX_B39_42 gDNA Contig7176 7375 1016 Conyza canadensis PPOX_B39_43 gDNA Contig 7351 7550 1017Conyza canadensis PPOX_B39_43 gDNA Contig 7351 7550 1018 Conyzacanadensis PPOX_B39_44 gDNA Contig 7526 7725 1019 Conyza canadensisPPOX_B39_44 gDNA Contig 7526 7725 1020 Conyza canadensis PPOX_B39_45gDNA Contig 7701 7900 1021 Conyza canadensis PPOX_B39_45 gDNA Contig7701 7900 1022 Conyza canadensis PPOX_B39_46 gDNA Contig 7876 8075 1023Conyza canadensis PPOX_B39_46 gDNA Contig 7876 8075 1024 Conyzacanadensis PPOX_B39_47 gDNA Contig 8051 8250 1025 Conyza canadensisPPOX_B39_47 gDNA Contig 8051 8250 1026 Conyza canadensis PPOX_B39_48gDNA Contig 8226 8425 1027 Conyza canadensis PPOX_B39_48 gDNA Contig8226 8425 1028 Conyza canadensis PPOX_B39_49 gDNA Contig 8401 8600 1029Conyza canadensis PPOX_B39_49 gDNA Contig 8401 8600 1030 Conyzacanadensis PPOX_B39_50 gDNA Contig 8576 8775 1031 Conyza canadensisPPOX_B39_50 gDNA Contig 8576 8775 1032 Conyza canadensis PPOX_B39_51gDNA Contig 8751 8950 1033 Conyza canadensis PPOX_B39_51 gDNA Contig8751 8950 1034 Conyza canadensis PPOX_B39_52 gDNA Contig 8926 9125 1035Conyza canadensis PPOX_B39_52 gDNA Contig 8926 9125 1036 Conyzacanadensis PPOX_B39_53 gDNA Contig 9101 9300 1037 Conyza canadensisPPOX_B39_53 gDNA Contig 9101 9300 1038 Conyza canadensis PPOX_B39_54gDNA Contig 9276 9475 1039 Conyza canadensis PPOX_B39_54 gDNA Contig9276 9475 1040 Conyza canadensis PPOX_B39_55 gDNA Contig 9451 9650 1041Conyza canadensis PPOX_B39_55 gDNA Contig 9451 9650 1042 Conyzacanadensis PPOX_B39_56 gDNA Contig 9626 9825 1043 Conyza canadensisPPOX_B39_56 gDNA Contig 9626 9825 1044 Conyza canadensis PPOX_B39_57gDNA Contig 9801 10000 1045 Conyza canadensis PPOX_B39_57 gDNA Contig9801 10000 1046 Conyza canadensis PPOX_B39_58 gDNA Contig 9976 101751047 Conyza canadensis PPOX_B39_58 gDNA Contig 9976 10175 1048 Conyzacanadensis PPOX_B36_1 cDNA Contig 1 200 1049 Conyza canadensisPPOX_B36_1 cDNA Contig 1 200 1050 Conyza canadensis PPOX_B36_2 cDNAContig 176 375 1051 Conyza canadensis PPOX_B36_2 cDNA Contig 176 3751052 Conyza canadensis PPOX_B36_3 cDNA Contig 351 550 1053 Conyzacanadensis PPOX_B36_3 cDNA Contig 351 550 1054 Conyza canadensisPPOX_B36_4 cDNA Contig 526 725 1055 Conyza canadensis PPOX_B36_4 cDNAContig 526 725 1056 Conyza canadensis PPOX_B36_5 cDNA Contig 701 9001057 Conyza canadensis PPOX_B36_5 cDNA Contig 701 900 1058 Conyzacanadensis PPOX_B36_6 cDNA Contig 876 1075 1059 Conyza canadensisPPOX_B36_6 cDNA Contig 876 1075 1060 Conyza canadensis PPOX_B36_7 cDNAContig 1051 1250 1061 Conyza canadensis PPOX_B36_7 cDNA Contig 1051 12501062 Conyza canadensis PPOX_B36_8 cDNA Contig 1226 1425 1063 Conyzacanadensis PPOX_B36_8 cDNA Contig 1226 1425 1064 Conyza canadensisPPOX_B36_9 cDNA Contig 1401 1600 1065 Conyza canadensis PPOX_B36_9 cDNAContig 1401 1600 1066 Conyza canadensis PPOX_B40_1 gDNA Contig 1 2001067 Conyza canadensis PPOX_B40_1 gDNA Contig 1 200 1068 Conyzacanadensis PPOX_B40_2 gDNA Contig 176 375 1069 Conyza canadensisPPOX_B40_2 gDNA Contig 176 375 1070 Conyza canadensis PPOX_B40_3 gDNAContig 351 550 1071 Conyza canadensis PPOX_B40_3 gDNA Contig 351 5501072 Conyza canadensis PPOX_B40_4 gDNA Contig 526 725 1073 Conyzacanadensis PPOX_B40_4 gDNA Contig 526 725 1074 Conyza canadensisPPOX_B40_5 gDNA Contig 701 900 1075 Conyza canadensis PPOX_B40_5 gDNAContig 701 900 1076 Conyza canadensis PPOX_B40_6 gDNA Contig 876 10751077 Conyza canadensis PPOX_B40_6 gDNA Contig 876 1075 1078 Conyzacanadensis PPOX_B40_7 gDNA Contig 1051 1250 1079 Conyza canadensisPPOX_B40_7 gDNA Contig 1051 1250 1080 Conyza canadensis PPOX_B40_8 gDNAContig 1226 1425 1081 Conyza canadensis PPOX_B40_8 gDNA Contig 1226 14251082 Conyza canadensis PPOX_B40_9 gDNA Contig 1401 1600 1083 Conyzacanadensis PPOX_B40_9 gDNA Contig 1401 1600 1084 Conyza canadensisPPOX_B40_10 gDNA Contig 1576 1775 1085 Conyza canadensis PPOX_B40_10gDNA Contig 1576 1775 1086 Conyza canadensis PPOX_B40_11 gDNA Contig1751 1950 1087 Conyza canadensis PPOX_B40_11 gDNA Contig 1751 1950 1088Conyza canadensis PPOX_B40_12 gDNA Contig 1926 2125 1089 Conyzacanadensis PPOX_B40_12 gDNA Contig 1926 2125 1090 Conyza canadensisPPOX_B40_13 gDNA Contig 2101 2300 1091 Conyza canadensis PPOX_B40_13gDNA Contig 2101 2300 1092 Conyza canadensis PPOX_B40_14 gDNA Contig2276 2475 1093 Conyza canadensis PPOX_B40_14 gDNA Contig 2276 2475 1094Conyza canadensis PPOX_B37_1 cDNA Contig 1 200 1095 Conyza canadensisPPOX_B37_1 cDNA Contig 1 200 1096 Conyza canadensis PPOX_B37_2 cDNAContig 176 375 1097 Conyza canadensis PPOX_B37_2 cDNA Contig 176 3751098 Conyza canadensis PPOX_B37_3 cDNA Contig 351 550 1099 Conyzacanadensis PPOX_B37_3 cDNA Contig 351 550 1100 Digitaria sanguinalisPPOX_B54_1 cDNA Contig 1 200 1101 Digitaria sanguinalis PPOX_B54_1 cDNAContig 1 200 1102 Digitaria sanguinalis PPOX_B54_2 cDNA Contig 176 3751103 Digitaria sanguinalis PPOX_B54_2 cDNA Contig 176 375 1104 Digitariasanguinalis PPOX_B56_1 gDNA Contig 1 200 1105 Digitaria sanguinalisPPOX_B56_1 gDNA Contig 1 200 1106 Digitaria sanguinalis PPOX_B56_2 gDNAContig 176 375 1107 Digitaria sanguinalis PPOX_B56_2 gDNA Contig 176 3751108 Digitaria sanguinalis PPOX_B56_3 gDNA Contig 351 550 1109 Digitariasanguinalis PPOX_B56_3 gDNA Contig 351 550 1110 Digitaria sanguinalisPPOX_B56_4 gDNA Contig 526 725 1111 Digitaria sanguinalis PPOX_B56_4gDNA Contig 526 725 1112 Digitaria sanguinalis PPOX_B56_5 gDNA Contig701 900 1113 Digitaria sanguinalis PPOX_B56_5 gDNA Contig 701 900 1114Digitaria sanguinalis PPOX_B56_6 gDNA Contig 876 1075 1115 Digitariasanguinalis PPOX_B56_6 gDNA Contig 876 1075 1116 Digitaria sanguinalisPPOX_B56_7 gDNA Contig 1051 1250 1117 Digitaria sanguinalis PPOX_B56_7gDNA Contig 1051 1250 1118 Digitaria sanguinalis PPOX_B56_8 gDNA Contig1226 1425 1119 Digitaria sanguinalis PPOX_B56_8 gDNA Contig 1226 14251120 Digitaria sanguinalis PPOX_B56_9 gDNA Contig 1401 1600 1121Digitaria sanguinalis PPOX_B56_9 gDNA Contig 1401 1600 1122 Digitariasanguinalis PPOX_B56_10 gDNA Contig 1576 1775 1123 Digitaria sanguinalisPPOX_B56_10 gDNA Contig 1576 1775 1124 Digitaria sanguinalis PPOX_B56_11gDNA Contig 1751 1950 1125 Digitaria sanguinalis PPOX_B56_11 gDNA Contig1751 1950 1126 Digitaria sanguinalis PPOX_B56_12 gDNA Contig 1926 21251127 Digitaria sanguinalis PPOX_B56_12 gDNA Contig 1926 2125 1128Digitaria sanguinalis PPOX_B56_13 gDNA Contig 2101 2300 1129 Digitariasanguinalis PPOX_B56_13 gDNA Contig 2101 2300 1130 Digitaria sanguinalisPPOX_B56_14 gDNA Contig 2276 2475 1131 Digitaria sanguinalis PPOX_B56_14gDNA Contig 2276 2475 1132 Digitaria sanguinalis PPOX_B56_15 gDNA Contig2451 2650 1133 Digitaria sanguinalis PPOX_B56_15 gDNA Contig 2451 26501134 Digitaria sanguinalis PPOX_B53_1 cDNA Contig 1 200 1135 Digitariasanguinalis PPOX_B53_1 cDNA Contig 1 200 1136 Digitaria sanguinalisPPOX_B53_2 cDNA Contig 176 375 1137 Digitaria sanguinalis PPOX_B53_2cDNA Contig 176 375 1138 Digitaria sanguinalis PPOX_B53_3 cDNA Contig351 550 1139 Digitaria sanguinalis PPOX_B53_3 cDNA Contig 351 550 1140Digitaria sanguinalis PPOX_B53_4 cDNA Contig 526 725 1141 Digitariasanguinalis PPOX_B53_4 cDNA Contig 526 725 1142 Digitaria sanguinalisPPOX_B57_1 gDNA Contig 1 200 1143 Digitaria sanguinalis PPOX_B57_1 gDNAContig 1 200 1144 Digitaria sanguinalis PPOX_B57_2 gDNA Contig 176 3751145 Digitaria sanguinalis PPOX_B57_2 gDNA Contig 176 375 1146 Digitariasanguinalis PPOX_B57_3 gDNA Contig 351 550 1147 Digitaria sanguinalisPPOX_B57_3 gDNA Contig 351 550 1148 Digitaria sanguinalis PPOX_B57_4gDNA Contig 526 725 1149 Digitaria sanguinalis PPOX_B57_4 gDNA Contig526 725 1150 Digitaria sanguinalis PPOX_B57_5 gDNA Contig 701 900 1151Digitaria sanguinalis PPOX_B57_5 gDNA Contig 701 900 1152 Digitariasanguinalis PPOX_B57_6 gDNA Contig 876 1075 1153 Digitaria sanguinalisPPOX_B57_6 gDNA Contig 876 1075 1154 Digitaria sanguinalis PPOX_B55_1cDNA Contig 1 200 1155 Digitaria sanguinalis PPOX_B55_1 cDNA Contig 1200 1156 Digitaria sanguinalis PPOX_B55_2 cDNA Contig 176 375 1157Digitaria sanguinalis PPOX_B55_2 cDNA Contig 176 375 1158 Euphorbiaheterophylla PPOX_B47_1 gDNA Contig 1 121 1159 Euphorbia heterophyllaPPOX_B47_1 gDNA Contig 1 121 1160 Euphorbia heterophylla PPOX_B42_1 cDNAContig 1 200 1161 Euphorbia heterophylla PPOX_B42_1 cDNA Contig 1 2001162 Euphorbia heterophylla PPOX_B42_2 cDNA Contig 176 375 1163Euphorbia heterophylla PPOX_B42_2 cDNA Contig 176 375 1164 Euphorbiaheterophylla PPOX_B46_1 gDNA Contig 1 200 1165 Euphorbia heterophyllaPPOX_B46_1 gDNA Contig 1 200 1166 Euphorbia heterophylla PPOX_B46_2 gDNAContig 176 375 1167 Euphorbia heterophylla PPOX_B46_2 gDNA Contig 176375 1168 Euphorbia heterophylla PPOX_B46_3 gDNA Contig 351 550 1169Euphorbia heterophylla PPOX_B46_3 gDNA Contig 351 550 1170 Euphorbiaheterophylla PPOX_B46_4 gDNA Contig 526 725 1171 Euphorbia heterophyllaPPOX_B46_4 gDNA Contig 526 725 1172 Euphorbia heterophylla PPOX_B45_1gDNA Contig 1 200 1173 Euphorbia heterophylla PPOX_B45_1 gDNA Contig 1200 1174 Euphorbia heterophylla PPOX_B45_2 gDNA Contig 176 375 1175Euphorbia heterophylla PPOX_B45_2 gDNA Contig 176 375 1176 Euphorbiaheterophylla PPOX_B45_3 gDNA Contig 351 550 1177 Euphorbia heterophyllaPPOX_B45_3 gDNA Contig 351 550 1178 Euphorbia heterophylla PPOX_B45_4gDNA Contig 526 725 1179 Euphorbia heterophylla PPOX_B45_4 gDNA Contig526 725 1180 Euphorbia heterophylla PPOX_B45_5 gDNA Contig 701 900 1181Euphorbia heterophylla PPOX_B45_5 gDNA Contig 701 900 1182 Euphorbiaheterophylla PPOX_B41_1 cDNA Contig 1 200 1183 Euphorbia heterophyllaPPOX_B41_1 cDNA Contig 1 200 1184 Euphorbia heterophylla PPOX_B41_2 cDNAContig 176 375 1185 Euphorbia heterophylla PPOX_B41_2 cDNA Contig 176375 1186 Euphorbia heterophylla PPOX_B41_3 cDNA Contig 351 550 1187Euphorbia heterophylla PPOX_B41_3 cDNA Contig 351 550 1188 Euphorbiaheterophylla PPOX_B41_4 cDNA Contig 526 725 1189 Euphorbia heterophyllaPPOX_B41_4 cDNA Contig 526 725 1190 Euphorbia heterophylla PPOX_B41_5cDNA Contig 701 900 1191 Euphorbia heterophylla PPOX_B41_5 cDNA Contig701 900 1192 Euphorbia heterophylla PPOX_B41_6 cDNA Contig 876 1075 1193Euphorbia heterophylla PPOX_B41_6 cDNA Contig 876 1075 1194 Euphorbiaheterophylla PPOX_B41_7 cDNA Contig 1051 1250 1195 Euphorbiaheterophylla PPOX_B41_7 cDNA Contig 1051 1250 1196 Euphorbiaheterophylla PPOX_B41_8 cDNA Contig 1226 1425 1197 Euphorbiaheterophylla PPOX_B41_8 cDNA Contig 1226 1425 1198 Euphorbiaheterophylla PPOX_B41_9 cDNA Contig 1401 1600 1199 Euphorbiaheterophylla PPOX_B41_9 cDNA Contig 1401 1600 1200 Euphorbiaheterophylla PPOX_B44_1 gDNA Contig 1 200 1201 Euphorbia heterophyllaPPOX_B44_1 gDNA Contig 1 200 1202 Euphorbia heterophylla PPOX_B44_2 gDNAContig 176 375 1203 Euphorbia heterophylla PPOX_B44_2 gDNA Contig 176375 1204 Euphorbia heterophylla PPOX_B44_3 gDNA Contig 351 550 1205Euphorbia heterophylla PPOX_B44_3 gDNA Contig 351 550 1206 Euphorbiaheterophylla PPOX_B44_4 gDNA Contig 526 725 1207 Euphorbia heterophyllaPPOX_B44_4 gDNA Contig 526 725 1208 Euphorbia heterophylla PPOX_B44_5gDNA Contig 701 900 1209 Euphorbia heterophylla PPOX_B44_5 gDNA Contig701 900 1210 Euphorbia heterophylla PPOX_B44_6 gDNA Contig 876 1075 1211Euphorbia heterophylla PPOX_B44_6 gDNA Contig 876 1075 1212 Euphorbiaheterophylla PPOX_B44_7 gDNA Contig 1051 1250 1213 Euphorbiaheterophylla PPOX_B44_7 gDNA Contig 1051 1250 1214 Euphorbiaheterophylla PPOX_B44_8 gDNA Contig 1226 1425 1215 Euphorbiaheterophylla PPOX_B44_8 gDNA Contig 1226 1425 1216 Euphorbiaheterophylla PPOX_B44_9 gDNA Contig 1401 1600 1217 Euphorbiaheterophylla PPOX_B44_9 gDNA Contig 1401 1600 1218 Euphorbiaheterophylla PPOX_B44_10 gDNA Contig 1576 1775 1219 Euphorbiaheterophylla PPOX_B44_10 gDNA Contig 1576 1775 1220 Euphorbiaheterophylla PPOX_B44_11 gDNA Contig 1751 1950 1221 Euphorbiaheterophylla PPOX_B44_11 gDNA Contig 1751 1950 1222 Euphorbiaheterophylla PPOX_B44_12 gDNA Contig 1926 2125 1223 Euphorbiaheterophylla PPOX_B44_12 gDNA Contig 1926 2125 1224 Euphorbiaheterophylla PPOX_B44_13 gDNA Contig 2101 2300 1225 Euphorbiaheterophylla PPOX_B44_13 gDNA Contig 2101 2300 1226 Euphorbiaheterophylla PPOX_B44_14 gDNA Contig 2276 2475 1227 Euphorbiaheterophylla PPOX_B44_14 gDNA Contig 2276 2475 1228 Euphorbiaheterophylla PPOX_B43_1 gDNA Contig 1 200 1229 Euphorbia heterophyllaPPOX_B43_1 gDNA Contig 1 200 1230 Euphorbia heterophylla PPOX_B43_2 gDNAContig 176 375 1231 Euphorbia heterophylla PPOX_B43_2 gDNA Contig 176375 1232 Euphorbia heterophylla PPOX_B43_3 gDNA Contig 351 550 1233Euphorbia heterophylla PPOX_B43_3 gDNA Contig 351 550 1234 Euphorbiaheterophylla PPOX_B43_4 gDNA Contig 526 725 1235 Euphorbia heterophyllaPPOX_B43_4 gDNA Contig 526 725 1236 Euphorbia heterophylla PPOX_B43_5gDNA Contig 701 900 1237 Euphorbia heterophylla PPOX_B43_5 gDNA Contig701 900 1238 Euphorbia heterophylla PPOX_B43_6 gDNA Contig 876 1075 1239Euphorbia heterophylla PPOX_B43_6 gDNA Contig 876 1075 1240 Euphorbiaheterophylla PPOX_B43_7 gDNA Contig 1051 1250 1241 Euphorbiaheterophylla PPOX_B43_7 gDNA Contig 1051 1250 1242 Euphorbiaheterophylla PPOX_B43_8 gDNA Contig 1226 1425 1243 Euphorbiaheterophylla PPOX_B43_8 gDNA Contig 1226 1425 1244 Euphorbiaheterophylla PPOX_B43_9 gDNA Contig 1401 1600 1245 Euphorbiaheterophylla PPOX_B43_9 gDNA Contig 1401 1600 1246 Euphorbiaheterophylla PPOX_B43_10 gDNA Contig 1576 1775 1247 Euphorbiaheterophylla PPOX_B43_10 gDNA Contig 1576 1775 1248 Euphorbiaheterophylla PPOX_B43_11 gDNA Contig 1751 1950 1249 Euphorbiaheterophylla PPOX_B43_11 gDNA Contig 1751 1950 1250 Euphorbiaheterophylla PPOX_B43_12 gDNA Contig 1926 2125 1251 Euphorbiaheterophylla PPOX_B43_12 gDNA Contig 1926 2125 1252 Euphorbiaheterophylla PPOX_B43_13 gDNA Contig 2101 2300 1253 Euphorbiaheterophylla PPOX_B43_13 gDNA Contig 2101 2300 1254 Euphorbiaheterophylla PPOX_B43_14 gDNA Contig 2276 2475 1255 Euphorbiaheterophylla PPOX_B43_14 gDNA Contig 2276 2475 1256 Euphorbiaheterophylla PPOX_B43_15 gDNA Contig 2451 2650 1257 Euphorbiaheterophylla PPOX_B43_15 gDNA Contig 2451 2650 1258 Euphorbiaheterophylla PPOX_B43_16 gDNA Contig 2626 2825 1259 Euphorbiaheterophylla PPOX_B43_16 gDNA Contig 2626 2825 1260 Euphorbiaheterophylla PPOX_B43_17 gDNA Contig 2801 3000 1261 Euphorbiaheterophylla PPOX_B43_17 gDNA Contig 2801 3000 1262 Euphorbiaheterophylla PPOX_B43_18 gDNA Contig 2976 3175 1263 Euphorbiaheterophylla PPOX_B43_18 gDNA Contig 2976 3175 1264 Euphorbiaheterophylla PPOX_B43_19 gDNA Contig 3151 3350 1265 Euphorbiaheterophylla PPOX_B43_19 gDNA Contig 3151 3350 1266 Euphorbiaheterophylla PPOX_B43_20 gDNA Contig 3326 3525 1267 Euphorbiaheterophylla PPOX_B43_20 gDNA Contig 3326 3525 1268 Euphorbiaheterophylla PPOX_B43_21 gDNA Contig 3501 3700 1269 Euphorbiaheterophylla PPOX_B43_21 gDNA Contig 3501 3700 1270 Euphorbiaheterophylla PPOX_B43_22 gDNA Contig 3676 3875 1271 Euphorbiaheterophylla PPOX_B43_22 gDNA Contig 3676 3875 1272 Euphorbiaheterophylla PPOX_B43_23 gDNA Contig 3851 4050 1273 Euphorbiaheterophylla PPOX_B43_23 gDNA Contig 3851 4050 1274 Euphorbiaheterophylla PPOX_B48_1 gDNA Contig 1 77 1275 Euphorbia heterophyllaPPOX_B48_1 gDNA Contig 1 77 1276 Kochia scoparia PPOX_B58_1 cDNA Contig1 200 1277 Kochia scoparia PPOX_B58_1 cDNA Contig 1 200 1278 Kochiascoparia PPOX_B58_2 cDNA Contig 176 375 1279 Kochia scoparia PPOX_B58_2cDNA Contig 176 375 1280 Kochia scoparia PPOX_B58_3 cDNA Contig 351 5501281 Kochia scoparia PPOX_B58_3 cDNA Contig 351 550 1282 Kochia scopariaPPOX_B58_4 cDNA Contig 526 725 1283 Kochia scoparia PPOX_B58_4 cDNAContig 526 725 1284 Kochia scoparia PPOX_B58_5 cDNA Contig 701 900 1285Kochia scoparia PPOX_B58_5 cDNA Contig 701 900 1286 Kochia scopariaPPOX_B58_6 cDNA Contig 876 1075 1287 Kochia scoparia PPOX_B58_6 cDNAContig 876 1075 1288 Kochia scoparia PPOX_B58_7 cDNA Contig 1051 12501289 Kochia scoparia PPOX_B58_7 cDNA Contig 1051 1250 1290 Kochiascoparia PPOX_B58_8 cDNA Contig 1226 1425 1291 Kochia scopariaPPOX_B58_8 cDNA Contig 1226 1425 1292 Kochia scoparia PPOX_B59_1 cDNAContig 1 200 1293 Kochia scoparia PPOX_B59_1 cDNA Contig 1 200 1294Kochia scoparia PPOX_B59_2 cDNA Contig 176 375 1295 Kochia scopariaPPOX_B59_2 cDNA Contig 176 375 1296 Kochia scoparia PPOX_B59_3 cDNAContig 351 550 1297 Kochia scoparia PPOX_B59_3 cDNA Contig 351 550 1298Kochia scoparia PPOX_B59_4 cDNA Contig 526 725 1299 Kochia scopariaPPOX_B59_4 cDNA Contig 526 725 1300 Kochia scoparia PPOX_B59_5 cDNAContig 701 900 1301 Kochia scoparia PPOX_B59_5 cDNA Contig 701 900 1302Kochia scoparia PPOX_B60_1 cDNA Contig 1 200 1303 Kochia scopariaPPOX_B60_1 cDNA Contig 1 200 1304 Kochia scoparia PPOX_B60_2 cDNA Contig176 375 1305 Kochia scoparia PPOX_B60_2 cDNA Contig 176 375 1306 Kochiascoparia PPOX_B60_3 cDNA Contig 351 550 1307 Kochia scoparia PPOX_B60_3cDNA Contig 351 550 1308 Kochia scoparia PPOX_B61_1 cDNA Contig 1 2001309 Kochia scoparia PPOX_B61_1 cDNA Contig 1 200 1310 Kochia scopariaPPOX_B61_2 cDNA Contig 176 375 1311 Kochia scoparia PPOX_B61_2 cDNAContig 176 375 1312 Lolium multiflorum PPOX_B65_1 cDNA Contig 1 150 1313Lolium multiflorum PPOX_B65_1 cDNA Contig 1 150 1314 Lolium multiflorumPPOX_B68_1 cDNA Contig 1 108 1315 Lolium multiflorum PPOX_B68_1 cDNAContig 1 108 1316 Lolium multiflorum PPOX_B66_1 cDNA Contig 1 144 1317Lolium multiflorum PPOX_B66_1 cDNA Contig 1 144 1318 Lolium multiflorumPPOX_B67_1 cDNA Contig 1 114 1319 Lolium multiflorum PPOX_B67_1 cDNAContig 1 114 1320 Lolium multiflorum PPOX_B69_1 gDNA Contig 1 200 1321Lolium multiflorum PPOX_B69_1 gDNA Contig 1 200 1322 Lolium multiflorumPPOX_B69_2 gDNA Contig 176 375 1323 Lolium multiflorum PPOX_B69_2 gDNAContig 176 375 1324 Lolium multiflorum PPOX_B69_3 gDNA Contig 351 5501325 Lolium multiflorum PPOX_B69_3 gDNA Contig 351 550 1326 Loliummultiflorum PPOX_B69_4 gDNA Contig 526 725 1327 Lolium multiflorumPPOX_B69_4 gDNA Contig 526 725 1328 Lolium multiflorum PPOX_B69_5 gDNAContig 701 900 1329 Lolium multiflorum PPOX_B69_5 gDNA Contig 701 9001330 Lolium multiflorum PPOX_B69_6 gDNA Contig 876 1075 1331 Loliummultiflorum PPOX_B69_6 gDNA Contig 876 1075 1332 Lolium multiflorumPPOX_B69_7 gDNA Contig 1051 1250 1333 Lolium multiflorum PPOX_B69_7 gDNAContig 1051 1250 1334 Lolium multiflorum PPOX_B69_8 gDNA Contig 12261425 1335 Lolium multiflorum PPOX_B69_8 gDNA Contig 1226 1425 1336Lolium multiflorum PPOX_B69_9 gDNA Contig 1401 1600 1337 Loliummultiflorum PPOX_B69_9 gDNA Contig 1401 1600 1338 Lolium multiflorumPPOX_B69_10 gDNA Contig 1576 1775 1339 Lolium multiflorum PPOX_B69_10gDNA Contig 1576 1775 1340 Lolium multiflorum PPOX_B69_11 gDNA Contig1751 1950 1341 Lolium multiflorum PPOX_B69_11 gDNA Contig 1751 1950 1342Lolium multiflorum PPOX_B69_12 gDNA Contig 1926 2125 1343 Loliummultiflorum PPOX_B69_12 gDNA Contig 1926 2125 1344 Lolium multiflorumPPOX_B69_13 gDNA Contig 2101 2300 1345 Lolium multiflorum PPOX_B69_13gDNA Contig 2101 2300 1346 Lolium multiflorum PPOX_B69_14 gDNA Contig2276 2475 1347 Lolium multiflorum PPOX_B69_14 gDNA Contig 2276 2475 1348Lolium multiflorum PPOX_B69_15 gDNA Contig 2451 2650 1349 Loliummultiflorum PPOX_B69_15 gDNA Contig 2451 2650 1350 Lolium multiflorumPPOX_B69_16 gDNA Contig 2626 2825 1351 Lolium multiflorum PPOX_B69_16gDNA Contig 2626 2825 1352 Lolium multiflorum PPOX_B69_17 gDNA Contig2801 3000 1353 Lolium multiflorum PPOX_B69_17 gDNA Contig 2801 3000 1354Lolium multiflorum PPOX_B62_1 cDNA Contig 1 200 1355 Lolium multiflorumPPOX_B62_1 cDNA Contig 1 200 1356 Lolium multiflorum PPOX_B62_2 cDNAContig 176 375 1357 Lolium multiflorum PPOX_B62_2 cDNA Contig 176 3751358 Lolium multiflorum PPOX_B62_3 cDNA Contig 351 550 1359 Loliummultiflorum PPOX_B62_3 cDNA Contig 351 550 1360 Lolium multiflorumPPOX_B62_4 cDNA Contig 526 725 1361 Lolium multiflorum PPOX_B62_4 cDNAContig 526 725 1362 Lolium multiflorum PPOX_B62_5 cDNA Contig 701 9001363 Lolium multiflorum PPOX_B62_5 cDNA Contig 701 900 1364 Loliummultiflorum PPOX_B62_6 cDNA Contig 876 1075 1365 Lolium multiflorumPPOX_B62_6 cDNA Contig 876 1075 1366 Lolium multiflorum PPOX_B70_1 gDNAContig 1 200 1367 Lolium multiflorum PPOX_B70_1 gDNA Contig 1 200 1368Lolium multiflorum PPOX_B70_2 gDNA Contig 176 375 1369 Loliummultiflorum PPOX_B70_2 gDNA Contig 176 375 1370 Lolium multiflorumPPOX_B70_3 gDNA Contig 351 550 1371 Lolium multiflorum PPOX_B70_3 gDNAContig 351 550 1372 Lolium multiflorum PPOX_B70_4 gDNA Contig 526 7251373 Lolium multiflorum PPOX_B70_4 gDNA Contig 526 725 1374 Loliummultiflorum PPOX_B71_1 gDNA Contig 1 200 1375 Lolium multiflorumPPOX_B71_1 gDNA Contig 1 200 1376 Lolium multiflorum PPOX_B71_2 gDNAContig 176 375 1377 Lolium multiflorum PPOX_B71_2 gDNA Contig 176 3751378 Lolium multiflorum PPOX_B63_1 cDNA Contig 1 200 1379 Loliummultiflorum PPOX_B63_1 cDNA Contig 1 200 1380 Lolium multiflorumPPOX_B64_1 cDNA Contig 1 200 1381 Lolium multiflorum PPOX_B64_1 cDNAContig 1 200

The gene sequences and fragments of Table 1 were compared and 21-mers ofcontiguous polynucleotides were identified that had homology across thevarious PPG oxidase gene sequences. The purpose is to identify triggermolecules that are useful as herbicidal molecules or in combination witha PPG oxidase inhibitor herbicide across a broad range of weed species.The sequences shown in Table 3 represent the 21-mers that were presentin the PPG oxidase gene of at least eight of the weed species ofTable 1. It is contemplated that additional 21-mers can be selected fromthe sequences of Table 1 that are specific for a single weed species ora few weeds species within a genus or trigger molecules that are atleast 18 contiguous nucleotides, at least 19 contiguous nucleotides, atleast 20 contiguous nucleotides or at least 21 contiguous nucleotides inlength and at least 85 percent identical to a PPG oxidase gene sequenceselected from the group consisting of SEQ ID NOs:1-71 or fragmentthereof.

By this method, it is possible to identify an oligonucleotide or severaloligonucleotides that are the most efficacious trigger molecule toeffect plant sensitivity to PPG oxidase inhibitor or modulation of PPGoxidase gene expression. The modulation of PPG oxidase gene expressionis determined by the detection of PPG oxidase siRNA molecules specificto PPG oxidase gene or by an observation of a reduction in the amount ofPPG oxidase RNA transcript produced relative to an untreated plant.Detection of siRNA can be accomplished, for example, using kits such asmirVana (Ambion, Austin Tex.) and mirPremier (Sigma-Aldrich, St Louis,Mo.).

The target DNA sequence isolated from genomic (gDNA) and coding DNA(cDNA) from the various weedy plant species for the PPG oxidase gene andthe assembled contigs as set forth in SEQ ID Nos: 1-71 were divided intofragments as shown in Table 3 below and as set forth in SEQ ID NOs1382-2221.

TABLE 3 SEQ #Spe- ID NO Gene cies Species 1382 PPOX 10 Amaranthus albus,Amaranthus graecizans, Amaranthus hybridus, Amaranthus. Lividus,Amaranthus palmeri, Amaranthus rudis, Amaranthus spinosus, Amaranthusthunbergii, Amaranthus viridis (“Amaranthus sp.”), Commelina diffusa1383 PPOX 10 Amaranthus sp., Chenopodium album 1384 PPOX 10 Amaranthussp., Chenopodium album 1385 PPOX 10 Amaranthus sp., Commelina diffusa1386 PPOX 10 Amaranthus sp., Chenopodium album 1387 PPOX 10 Amaranthussp., Chenopodium album 1388 PPOX 10 Amaranthus sp., Chenopodium album1389 PPOX 10 Amaranthus sp., Commelina diffusa 1390 PPOX 10 Amaranthussp., Chenopodium album 1391 PPOX 10 Amaranthus sp., Chenopodium album1392 PPOX 10 Amaranthus sp., Chenopodium album 1393 PPOX 10 Amaranthussp., Chenopodium album 1394 PPOX 10 Amaranthus sp., Commelina diffusa1395 PPOX 10 Amaranthus sp., Chenopodium album 1396 PPOX 9 Amaranthussp. 1397 PPOX 9 Amaranthus sp. 1398 PPOX 9 Amaranthus sp. 1399 PPOX 9Amaranthus sp. 1400 PPOX 9 Amaranthus sp. 1401 PPOX 9 Amaranthus sp.1402 PPOX 9 Amaranthus sp. 1403 PPOX 9 Amaranthus sp. 1404 PPOX 9Amaranthus sp. 1405 PPOX 9 Amaranthus sp. 1406 PPOX 9 Amaranthus sp.1407 PPOX 9 Amaranthus sp. 1408 PPOX 9 Amaranthus sp. 1409 PPOX 9Amaranthus sp. 1410 PPOX 9 Amaranthus sp. 1411 PPOX 9 Amaranthus sp.1412 PPOX 9 Amaranthus sp. 1413 PPOX 9 Amaranthus sp. 1414 PPOX 9Amaranthus sp. 1415 PPOX 9 Amaranthus sp. 1416 PPOX 9 Amaranthus sp.1417 PPOX 9 Amaranthus sp. 1418 PPOX 9 Amaranthus sp. 1419 PPOX 9Amaranthus sp. 1420 PPOX 9 Amaranthus sp. 1421 PPOX 9 Amaranthus sp.1422 PPOX 9 Amaranthus sp. 1423 PPOX 9 Amaranthus sp. 1424 PPOX 9Amaranthus sp. 1425 PPOX 9 Amaranthus sp. 1426 PPOX 9 Amaranthus sp.1427 PPOX 9 Amaranthus sp. 1428 PPOX 9 Amaranthus sp. 1429 PPOX 9Amaranthus sp. 1430 PPOX 9 Amaranthus sp. 1431 PPOX 9 Amaranthus sp.1432 PPOX 9 Amaranthus sp. 1433 PPOX 9 Amaranthus sp. 1434 PPOX 9Amaranthus sp. 1435 PPOX 9 Amaranthus sp. 1436 PPOX 9 Amaranthus sp.1437 PPOX 9 Amaranthus sp. 1438 PPOX 9 Amaranthus sp. 1439 PPOX 9Amaranthus sp. 1440 PPOX 9 Amaranthus sp. 1441 PPOX 9 Amaranthus sp.1442 PPOX 9 Amaranthus sp. 1443 PPOX 9 Amaranthus sp. 1444 PPOX 9Amaranthus sp. 1445 PPOX 9 Amaranthus sp. 1446 PPOX 9 Amaranthus sp.1447 PPOX 9 Amaranthus sp. 1448 PPOX 9 Amaranthus sp. 1449 PPOX 9Amaranthus sp. 1450 PPOX 9 Amaranthus sp. 1451 PPOX 9 Amaranthus sp.1452 PPOX 9 Amaranthus sp. 1453 PPOX 9 Amaranthus sp. 1454 PPOX 9Amaranthus sp. 1455 PPOX 9 Amaranthus sp. 1456 PPOX 9 Amaranthus sp.1457 PPOX 9 Amaranthus sp. 1458 PPOX 9 Amaranthus sp. 1459 PPOX 9Amaranthus sp. 1460 PPOX 9 Amaranthus sp. 1461 PPOX 9 Amaranthus sp.1462 PPOX 9 Amaranthus sp. 1463 PPOX 9 Amaranthus sp. 1464 PPOX 9Amaranthus sp. 1465 PPOX 9 Amaranthus sp. 1466 PPOX 9 Amaranthus sp.1467 PPOX 9 Amaranthus sp. 1468 PPOX 9 Amaranthus sp. 1469 PPOX 9Amaranthus sp. 1470 PPOX 9 Amaranthus sp. 1471 PPOX 9 Amaranthus sp.1472 PPOX 9 Amaranthus sp. 1473 PPOX 9 Amaranthus sp. 1474 PPOX 9Amaranthus sp. 1475 PPOX 9 Amaranthus sp. 1476 PPOX 9 Amaranthus sp.1477 PPOX 9 Amaranthus sp. 1478 PPOX 9 Amaranthus sp. 1479 PPOX 9Amaranthus sp. 1480 PPOX 9 Amaranthus sp. 1481 PPOX 9 Amaranthus sp.1482 PPOX 9 Amaranthus sp. 1483 PPOX 9 Amaranthus sp. 1484 PPOX 9Amaranthus sp. 1485 PPOX 9 Amaranthus sp. 1486 PPOX 9 Amaranthus sp.1487 PPOX 9 Amaranthus sp. 1488 PPOX 9 Amaranthus sp. 1489 PPOX 9Amaranthus sp. 1490 PPOX 9 Amaranthus sp. 1491 PPOX 9 Amaranthus sp.1492 PPOX 9 Amaranthus sp. 1493 PPOX 9 Amaranthus sp. 1494 PPOX 9Amaranthus sp. 1495 PPOX 9 Amaranthus sp. 1496 PPOX 9 Amaranthus sp.1497 PPOX 9 Amaranthus sp. 1498 PPOX 9 Amaranthus sp. 1499 PPOX 9Amaranthus sp. 1500 PPOX 9 Amaranthus sp. 1501 PPOX 9 Amaranthus sp.1502 PPOX 9 Amaranthus sp. 1503 PPOX 9 Amaranthus sp. 1504 PPOX 9Amaranthus sp. 1505 PPOX 9 Amaranthus sp. 1506 PPOX 9 Amaranthus sp.1507 PPOX 9 Amaranthus sp. 1508 PPOX 9 Amaranthus sp. 1509 PPOX 9Amaranthus sp. 1510 PPOX 9 Amaranthus sp. 1511 PPOX 9 Amaranthus sp.1512 PPOX 9 Amaranthus sp. 1513 PPOX 9 Amaranthus sp. 1514 PPOX 9Amaranthus sp. 1515 PPOX 9 Amaranthus sp. 1516 PPOX 9 Amaranthus sp.1517 PPOX 9 Amaranthus sp. 1518 PPOX 9 Amaranthus sp. 1519 PPOX 9Amaranthus sp. 1520 PPOX 9 Amaranthus sp. 1521 PPOX 9 Amaranthus sp.1522 PPOX 9 Amaranthus sp. 1523 PPOX 9 Amaranthus sp. 1524 PPOX 9Amaranthus sp. 1525 PPOX 9 Amaranthus sp. 1526 PPOX 9 Amaranthus sp.1527 PPOX 9 Amaranthus sp. 1528 PPOX 9 Amaranthus sp. 1529 PPOX 9Amaranthus sp. 1530 PPOX 9 Amaranthus sp. 1531 PPOX 9 Amaranthus sp.1532 PPOX 9 Amaranthus sp. 1533 PPOX 9 Amaranthus sp. 1534 PPOX 9Amaranthus sp. 1535 PPOX 9 Amaranthus sp. 1536 PPOX 9 Amaranthus sp.1537 PPOX 9 Amaranthus sp. 1538 PPOX 9 Amaranthus sp. 1539 PPOX 9Amaranthus sp. 1540 PPOX 9 Amaranthus sp. 1541 PPOX 9 Amaranthus sp.1542 PPOX 9 Amaranthus sp. 1543 PPOX 9 Amaranthus sp. 1544 PPOX 9Amaranthus sp. 1545 PPOX 9 Amaranthus sp. 1546 PPOX 9 Amaranthus sp.1547 PPOX 9 Amaranthus sp. 1548 PPOX 9 Amaranthus sp. 1549 PPOX 9Amaranthus sp. 1550 PPOX 9 Amaranthus sp. 1551 PPOX 9 Amaranthus sp.1552 PPOX 9 Amaranthus sp. 1553 PPOX 9 Amaranthus sp. 1554 PPOX 9Amaranthus sp. 1555 PPOX 9 Amaranthus sp. 1556 PPOX 9 Amaranthus sp.1557 PPOX 9 Amaranthus sp. 1558 PPOX 9 Amaranthus sp. 1559 PPOX 9Amaranthus sp. 1560 PPOX 9 Amaranthus sp. 1561 PPOX 9 Amaranthus sp.1562 PPOX 9 Amaranthus sp. 1563 PPOX 9 Amaranthus sp. 1564 PPOX 9Amaranthus sp. 1565 PPOX 9 Amaranthus sp. 1566 PPOX 9 Amaranthus sp.1567 PPOX 9 Amaranthus sp. 1568 PPOX 9 Amaranthus sp. 1569 PPOX 9Amaranthus sp. 1570 PPOX 9 Amaranthus sp. 1571 PPOX 9 Amaranthus sp.1572 PPOX 9 Amaranthus sp. 1573 PPOX 9 Amaranthus sp. 1574 PPOX 9Amaranthus sp. 1575 PPOX 9 Amaranthus sp. 1576 PPOX 9 Amaranthus sp.1577 PPOX 9 Amaranthus sp. 1578 PPOX 9 Amaranthus sp. 1579 PPOX 9Amaranthus sp. 1580 PPOX 9 Amaranthus sp. 1581 PPOX 9 Amaranthus sp.1582 PPOX 9 Amaranthus sp. 1583 PPOX 9 Amaranthus sp. 1584 PPOX 9Amaranthus sp. 1585 PPOX 9 Amaranthus sp. 1586 PPOX 9 Amaranthus sp.1587 PPOX 9 Amaranthus sp. 1588 PPOX 9 Amaranthus sp. 1589 PPOX 9Amaranthus sp. 1590 PPOX 9 Amaranthus sp. 1591 PPOX 9 Amaranthus sp.1592 PPOX 9 Amaranthus sp. 1593 PPOX 9 Amaranthus sp. 1594 PPOX 9Amaranthus sp. 1595 PPOX 9 Amaranthus sp. 1596 PPOX 9 Amaranthus sp.1597 PPOX 9 Amaranthus sp. 1598 PPOX 9 Amaranthus sp. 1599 PPOX 9Amaranthus sp. 1600 PPOX 9 Amaranthus sp. 1601 PPOX 9 Amaranthus sp.1602 PPOX 9 Amaranthus sp. 1603 PPOX 9 Amaranthus sp. 1604 PPOX 9Amaranthus sp. 1605 PPOX 9 Amaranthus sp. 1606 PPOX 9 Amaranthus sp.1607 PPOX 9 Amaranthus sp. 1608 PPOX 9 Amaranthus sp. 1609 PPOX 9Amaranthus sp. 1610 PPOX 9 Amaranthus sp. 1611 PPOX 9 Amaranthus sp.1612 PPOX 9 Amaranthus sp. 1613 PPOX 9 Amaranthus sp. 1614 PPOX 9Amaranthus sp. 1615 PPOX 9 Amaranthus sp. 1616 PPOX 9 Amaranthus sp.1617 PPOX 9 Amaranthus sp. 1618 PPOX 9 Amaranthus sp. 1619 PPOX 9Amaranthus sp. 1620 PPOX 9 Amaranthus sp. 1621 PPOX 9 Amaranthus sp.1622 PPOX 9 Amaranthus sp. 1623 PPOX 9 Amaranthus sp. 1624 PPOX 9Amaranthus sp. 1625 PPOX 9 Amaranthus sp. 1626 PPOX 9 Amaranthus sp.1627 PPOX 9 Amaranthus sp. 1628 PPOX 9 Amaranthus sp. 1629 PPOX 9Amaranthus sp. 1630 PPOX 9 Amaranthus sp. 1631 PPOX 9 Amaranthus sp.1632 PPOX 9 Amaranthus sp. 1633 PPOX 9 Amaranthus sp. 1634 PPOX 9Amaranthus sp. 1635 PPOX 9 Amaranthus sp. 1636 PPOX 9 Amaranthus sp.1637 PPOX 9 Amaranthus sp. 1638 PPOX 9 Amaranthus sp. 1639 PPOX 9Amaranthus sp. 1640 PPOX 9 Amaranthus sp. 1641 PPOX 9 Amaranthus sp.1642 PPOX 9 Amaranthus sp. 1643 PPOX 9 Amaranthus sp. 1644 PPOX 9Amaranthus sp. 1645 PPOX 9 Amaranthus sp. 1646 PPOX 9 Amaranthus sp.1647 PPOX 9 Amaranthus sp. 1648 PPOX 9 Amaranthus sp. 1649 PPOX 9Amaranthus sp. 1650 PPOX 9 Amaranthus sp. 1651 PPOX 9 Amaranthus sp.1652 PPOX 9 Amaranthus sp. 1653 PPOX 9 Amaranthus sp. 1654 PPOX 9Amaranthus sp. 1655 PPOX 9 Amaranthus sp. 1656 PPOX 9 Amaranthus sp.1657 PPOX 9 Amaranthus sp. 1658 PPOX 9 Amaranthus sp. 1659 PPOX 9Amaranthus sp. 1660 PPOX 9 Amaranthus sp. 1661 PPOX 9 Amaranthus sp.1662 PPOX 9 Amaranthus sp. 1663 PPOX 9 Amaranthus sp. 1664 PPOX 9Amaranthus sp. 1665 PPOX 9 Amaranthus sp. 1666 PPOX 9 Amaranthus sp.1667 PPOX 9 Amaranthus sp. 1668 PPOX 9 Amaranthus sp. 1669 PPOX 9Amaranthus sp. 1670 PPOX 9 Amaranthus sp. 1671 PPOX 9 Amaranthus sp.1672 PPOX 9 Amaranthus sp. 1673 PPOX 9 Amaranthus sp. 1674 PPOX 9Amaranthus sp. 1675 PPOX 9 Amaranthus sp. 1676 PPOX 9 Amaranthus sp.1677 PPOX 9 Amaranthus sp. 1678 PPOX 9 Amaranthus sp. 1679 PPOX 9Amaranthus sp. 1680 PPOX 9 Amaranthus sp. 1681 PPOX 9 Amaranthus sp.1682 PPOX 9 Amaranthus sp. 1683 PPOX 9 Amaranthus sp. 1684 PPOX 9Amaranthus sp. 1685 PPOX 9 Amaranthus sp. 1686 PPOX 9 Amaranthus sp.1687 PPOX 9 Amaranthus sp. 1688 PPOX 9 Amaranthus sp. 1689 PPOX 9Amaranthus sp. 1690 PPOX 9 Amaranthus sp. 1691 PPOX 9 Amaranthus sp.1692 PPOX 9 Amaranthus sp. 1693 PPOX 9 Amaranthus sp. 1694 PPOX 9Amaranthus sp. 1695 PPOX 9 Amaranthus sp. 1696 PPOX 9 Amaranthus sp.1697 PPOX 9 Amaranthus sp. 1698 PPOX 9 Amaranthus sp. 1699 PPOX 9Amaranthus sp. 1700 PPOX 9 Amaranthus sp. 1701 PPOX 9 Amaranthus sp.1702 PPOX 9 Amaranthus sp. 1703 PPOX 9 Amaranthus sp. 1704 PPOX 9Amaranthus sp. 1705 PPOX 9 Amaranthus sp. 1706 PPOX 9 Amaranthus sp.1707 PPOX 9 Amaranthus sp. 1708 PPOX 9 Amaranthus sp. 1709 PPOX 9Amaranthus sp. 1710 PPOX 9 Amaranthus sp. 1711 PPOX 9 Amaranthus sp.1712 PPOX 9 Amaranthus sp. 1713 PPOX 9 Amaranthus sp. 1714 PPOX 9Amaranthus sp. 1715 PPOX 9 Amaranthus sp. 1716 PPOX 9 Amaranthus sp.1717 PPOX 9 Amaranthus sp. 1718 PPOX 9 Amaranthus sp. 1719 PPOX 9Amaranthus sp. 1720 PPOX 9 Amaranthus sp. 1721 PPOX 9 Amaranthus sp.1722 PPOX 9 Amaranthus sp. 1723 PPOX 9 Amaranthus sp. 1724 PPOX 9Amaranthus sp. 1725 PPOX 9 Amaranthus sp. 1726 PPOX 9 Amaranthus sp.1727 PPOX 9 Amaranthus sp. 1728 PPOX 9 Amaranthus sp. 1729 PPOX 9Amaranthus sp. 1730 PPOX 9 Amaranthus sp. 1731 PPOX 9 Amaranthus sp.1732 PPOX 9 Amaranthus sp. 1733 PPOX 9 Amaranthus sp. 1734 PPOX 9Amaranthus sp. 1735 PPOX 9 Amaranthus sp. 1736 PPOX 9 Amaranthus sp.1737 PPOX 9 Amaranthus sp. 1738 PPOX 9 Amaranthus sp. 1739 PPOX 9Amaranthus sp. 1740 PPOX 9 Amaranthus sp. 1741 PPOX 9 Amaranthus sp.1742 PPOX 9 Amaranthus sp. 1743 PPOX 9 Amaranthus sp. 1744 PPOX 9Amaranthus sp. 1745 PPOX 9 Amaranthus sp. 1746 PPOX 9 Amaranthus sp.1747 PPOX 9 Amaranthus sp. 1748 PPOX 9 Amaranthus sp. 1749 PPOX 9Amaranthus sp. 1750 PPOX 9 Amaranthus sp. 1751 PPOX 9 Amaranthus sp.1752 PPOX 9 Amaranthus sp. 1753 PPOX 9 Amaranthus sp. 1754 PPOX 9Amaranthus sp. 1755 PPOX 9 Amaranthus sp. 1756 PPOX 9 Amaranthus sp.1757 PPOX 9 Amaranthus sp. 1758 PPOX 9 Amaranthus sp. 1759 PPOX 9Amaranthus sp. 1760 PPOX 9 Amaranthus sp. 1761 PPOX 9 Amaranthus sp.1762 PPOX 9 Amaranthus sp. 1763 PPOX 9 Amaranthus sp. 1764 PPOX 9Amaranthus sp. 1765 PPOX 9 Amaranthus sp. 1766 PPOX 9 Amaranthus sp.1767 PPOX 9 Amaranthus sp. 1768 PPOX 9 Amaranthus sp. 1769 PPOX 9Amaranthus sp. 1770 PPOX 9 Amaranthus sp. 1771 PPOX 9 Amaranthus sp.1772 PPOX 9 Amaranthus sp. 1773 PPOX 9 Amaranthus sp. 1774 PPOX 9Amaranthus sp. 1775 PPOX 9 Amaranthus sp. 1776 PPOX 9 Amaranthus sp.1777 PPOX 9 Amaranthus sp. 1778 PPOX 9 Amaranthus sp. 1779 PPOX 9Amaranthus sp. 1780 PPOX 9 Amaranthus sp. 1781 PPOX 9 Amaranthus sp.1782 PPOX 9 Amaranthus sp. 1783 PPOX 9 Amaranthus sp. 1784 PPOX 9Amaranthus sp. 1785 PPOX 9 Amaranthus sp. 1786 PPOX 9 Amaranthus sp.1787 PPOX 9 Amaranthus sp. 1788 PPOX 9 Amaranthus sp. 1789 PPOX 9Amaranthus sp. 1790 PPOX 9 Amaranthus sp. 1791 PPOX 9 Amaranthus sp.1792 PPOX 9 Amaranthus sp. 1793 PPOX 9 Amaranthus sp. 1794 PPOX 9Amaranthus sp. 1795 PPOX 9 Amaranthus sp. 1796 PPOX 9 Amaranthus sp.1797 PPOX 9 Amaranthus sp. 1798 PPOX 9 Amaranthus sp. 1799 PPOX 9Amaranthus sp. 1800 PPOX 9 Amaranthus sp. 1801 PPOX 9 Amaranthus sp.1802 PPOX 9 Amaranthus sp. 1803 PPOX 9 Amaranthus sp. 1804 PPOX 9Amaranthus sp. 1805 PPOX 9 Amaranthus sp. 1806 PPOX 9 Amaranthus sp.1807 PPOX 9 Amaranthus sp. 1808 PPOX 9 Amaranthus sp. 1809 PPOX 9Amaranthus sp. 1810 PPOX 9 Amaranthus sp. 1811 PPOX 9 Amaranthus sp.1812 PPOX 9 Amaranthus sp. 1813 PPOX 9 Amaranthus sp. 1814 PPOX 9Amaranthus sp. 1815 PPOX 9 Amaranthus sp. 1816 PPOX 9 Amaranthus sp.1817 PPOX 9 Amaranthus sp. 1818 PPOX 9 Amaranthus sp. 1819 PPOX 9Amaranthus sp. 1820 PPOX 9 Amaranthus sp. 1821 PPOX 9 Amaranthus sp.1822 PPOX 9 Amaranthus sp. 1823 PPOX 9 Amaranthus sp. 1824 PPOX 9Amaranthus sp. 1825 PPOX 9 Amaranthus sp. 1826 PPOX 9 Amaranthus sp.1827 PPOX 9 Amaranthus sp. 1828 PPOX 9 Amaranthus sp. 1829 PPOX 9Amaranthus sp. 1830 PPOX 9 Amaranthus sp. 1831 PPOX 9 Amaranthus sp.1832 PPOX 9 Amaranthus sp. 1833 PPOX 9 Amaranthus sp. 1834 PPOX 9Amaranthus sp. 1835 PPOX 9 Amaranthus sp. 1836 PPOX 9 Amaranthus sp.1837 PPOX 9 Amaranthus sp. 1838 PPOX 9 Amaranthus sp. 1839 PPOX 9Amaranthus sp. 1840 PPOX 9 Amaranthus sp. 1841 PPOX 9 Amaranthus sp.1842 PPOX 9 Amaranthus sp. 1843 PPOX 9 Amaranthus sp. 1844 PPOX 9Amaranthus sp. 1845 PPOX 9 Amaranthus sp. 1846 PPOX 9 Amaranthus sp.1847 PPOX 9 Amaranthus sp. 1848 PPOX 9 Amaranthus sp. 1849 PPOX 9Amaranthus sp. 1850 PPOX 9 Amaranthus sp. 1851 PPOX 9 Amaranthus sp.1852 PPOX 9 Amaranthus sp. 1853 PPOX 9 Amaranthus sp. 1854 PPOX 9Amaranthus sp. 1855 PPOX 9 Amaranthus sp. 1856 PPOX 9 Amaranthus sp.1857 PPOX 9 Amaranthus sp. 1858 PPOX 9 Amaranthus sp. 1859 PPOX 9Amaranthus sp. 1860 PPOX 9 Amaranthus sp. 1861 PPOX 9 Amaranthus sp.1862 PPOX 9 Amaranthus sp. 1863 PPOX 9 Amaranthus sp. 1864 PPOX 9Amaranthus sp. 1865 PPOX 9 Amaranthus sp. 1866 PPOX 9 Amaranthus sp.1867 PPOX 9 Amaranthus sp. 1868 PPOX 9 Amaranthus sp. 1869 PPOX 9Amaranthus sp. 1870 PPOX 9 Amaranthus sp. 1871 PPOX 9 Amaranthus sp.1872 PPOX 9 Amaranthus sp. 1873 PPOX 9 Amaranthus sp. 1874 PPOX 9Amaranthus sp. 1875 PPOX 9 Amaranthus sp. 1876 PPOX 9 Amaranthus sp.1877 PPOX 9 Amaranthus sp. 1878 PPOX 9 Amaranthus sp. 1879 PPOX 9Amaranthus sp. 1880 PPOX 9 Amaranthus sp. 1881 PPOX 9 Amaranthus sp.1882 PPOX 9 Amaranthus sp. 1883 PPOX 9 Amaranthus sp. 1884 PPOX 9Amaranthus sp. 1885 PPOX 9 Amaranthus sp. 1886 PPOX 9 Amaranthus sp.1887 PPOX 9 Amaranthus sp. 1888 PPOX 9 Amaranthus sp. 1889 PPOX 9Amaranthus sp. 1890 PPOX 9 Amaranthus sp. 1891 PPOX 9 Amaranthus sp.1892 PPOX 9 Amaranthus sp. 1893 PPOX 9 Amaranthus sp. 1894 PPOX 9Amaranthus sp. 1895 PPOX 9 Amaranthus sp. 1896 PPOX 9 Amaranthus sp.1897 PPOX 9 Amaranthus sp. 1898 PPOX 9 Amaranthus sp. 1899 PPOX 9Amaranthus sp. 1900 PPOX 9 Amaranthus sp. 1901 PPOX 9 Amaranthus sp.1902 PPOX 9 Amaranthus sp. 1903 PPOX 9 Amaranthus sp. 1904 PPOX 9Amaranthus sp. 1905 PPOX 9 Amaranthus sp. 1906 PPOX 9 Amaranthus sp.1907 PPOX 9 Amaranthus sp. 1908 PPOX 9 Amaranthus sp. 1909 PPOX 9Amaranthus sp. 1910 PPOX 9 Amaranthus sp. 1911 PPOX 9 Amaranthus sp.1912 PPOX 9 Amaranthus sp. 1913 PPOX 9 Amaranthus sp. 1914 PPOX 9Amaranthus sp. 1915 PPOX 9 Amaranthus sp. 1916 PPOX 9 Amaranthus sp.1917 PPOX 9 Amaranthus sp. 1918 PPOX 9 Amaranthus sp. 1919 PPOX 9Amaranthus sp. 1920 PPOX 9 Amaranthus sp. 1921 PPOX 9 Amaranthus sp.1922 PPOX 9 Amaranthus sp. 1923 PPOX 9 Amaranthus sp. 1924 PPOX 9Amaranthus sp. 1925 PPOX 9 Amaranthus sp. 1926 PPOX 9 Amaranthus sp.1927 PPOX 9 Amaranthus sp. 1928 PPOX 9 Amaranthus sp. 1929 PPOX 9Amaranthus sp. 1930 PPOX 9 Amaranthus sp. 1931 PPOX 9 Amaranthus sp.1932 PPOX 9 Amaranthus sp. 1933 PPOX 9 Amaranthus sp. 1934 PPOX 9Amaranthus sp. 1935 PPOX 9 Amaranthus sp. 1936 PPOX 9 Amaranthus sp.1937 PPOX 9 Amaranthus sp. 1938 PPOX 9 Amaranthus sp. 1939 PPOX 9Amaranthus sp. 1940 PPOX 9 Amaranthus sp. 1941 PPOX 9 Amaranthus sp.1942 PPOX 9 Amaranthus sp. 1943 PPOX 9 Amaranthus sp. 1944 PPOX 9Amaranthus sp. 1945 PPOX 9 Amaranthus sp. 1946 PPOX 9 Amaranthus sp.1947 PPOX 9 Amaranthus sp. 1948 PPOX 9 Amaranthus sp. 1949 PPOX 9Amaranthus sp. 1950 PPOX 9 Amaranthus sp. 1951 PPOX 9 Amaranthus sp.1952 PPOX 9 Amaranthus sp. 1953 PPOX 9 Amaranthus sp. 1954 PPOX 9Amaranthus sp. 1955 PPOX 9 Amaranthus sp. 1956 PPOX 9 Amaranthus sp.1957 PPOX 9 Amaranthus sp. 1958 PPOX 9 Amaranthus sp. 1959 PPOX 9Amaranthus sp. 1960 PPOX 9 Amaranthus sp. 1961 PPOX 9 Amaranthus sp.1962 PPOX 9 Amaranthus sp. 1963 PPOX 9 Amaranthus sp. 1964 PPOX 9Amaranthus sp. 1965 PPOX 9 Amaranthus sp. 1966 PPOX 9 Amaranthus sp.1967 PPOX 9 Amaranthus sp. 1968 PPOX 9 Amaranthus sp. 1969 PPOX 9Amaranthus sp. 1970 PPOX 9 Amaranthus sp. 1971 PPOX 9 Amaranthus sp.1972 PPOX 9 Amaranthus sp. 1973 PPOX 9 Amaranthus sp. 1974 PPOX 9Amaranthus sp. 1975 PPOX 9 Amaranthus sp. 1976 PPOX 9 Amaranthus sp.1977 PPOX 9 Amaranthus sp. 1978 PPOX 9 Amaranthus sp. 1979 PPOX 9Amaranthus sp. 1980 PPOX 9 Amaranthus sp. 1981 PPOX 9 Amaranthus sp.1982 PPOX 9 Amaranthus sp. 1983 PPOX 9 Amaranthus sp. 1984 PPOX 9Amaranthus sp. 1985 PPOX 9 Amaranthus sp. 1986 PPOX 9 Amaranthus sp.1987 PPOX 9 Amaranthus sp. 1988 PPOX 9 Amaranthus sp. 1989 PPOX 9Amaranthus sp. 1990 PPOX 9 Amaranthus sp. 1991 PPOX 9 Amaranthus sp.1992 PPOX 9 Amaranthus sp. 1993 PPOX 9 Amaranthus sp. 1994 PPOX 9Amaranthus sp. 1995 PPOX 9 Amaranthus sp. 1996 PPOX 9 Amaranthus sp.1997 PPOX 9 Amaranthus sp. 1998 PPOX 9 Amaranthus sp. 1999 PPOX 9Amaranthus sp. 2000 PPOX 9 Amaranthus sp. 2001 PPOX 9 Amaranthus sp.2002 PPOX 9 Amaranthus sp. 2003 PPOX 9 Amaranthus sp. 2004 PPOX 9Amaranthus sp. 2005 PPOX 9 Amaranthus sp. 2006 PPOX 9 Amaranthus sp.2007 PPOX 9 Amaranthus sp. 2008 PPOX 9 Amaranthus sp. 2009 PPOX 9Amaranthus sp. 2010 PPOX 9 Amaranthus sp. 2011 PPOX 9 Amaranthus sp.2012 PPOX 9 Amaranthus sp. 2013 PPOX 9 Amaranthus sp. 2014 PPOX 9Amaranthus sp. 2015 PPOX 9 Amaranthus sp. 2016 PPOX 9 Amaranthus sp.2017 PPOX 9 Amaranthus sp. 2018 PPOX 9 Amaranthus sp. 2019 PPOX 9Amaranthus sp. 2020 PPOX 9 Amaranthus sp. 2021 PPOX 9 Amaranthus sp.2022 PPOX 9 Amaranthus sp. 2023 PPOX 9 Amaranthus sp. 2024 PPOX 9Amaranthus sp. 2025 PPOX 9 Amaranthus sp. 2026 PPOX 9 Amaranthus sp.2027 PPOX 9 Amaranthus sp. 2028 PPOX 9 Amaranthus sp. 2029 PPOX 9Amaranthus sp. 2030 PPOX 9 Amaranthus sp. 2031 PPOX 9 Amaranthus sp.2032 PPOX 9 Amaranthus sp. 2033 PPOX 9 Amaranthus sp. 2034 PPOX 9Amaranthus sp. 2035 PPOX 9 Amaranthus sp. 2036 PPOX 9 Amaranthus sp.2037 PPOX 9 Amaranthus sp. 2038 PPOX 9 Amaranthus sp. 2039 PPOX 9Amaranthus sp. 2040 PPOX 9 Amaranthus sp. 2041 PPOX 9 Amaranthus sp.2042 PPOX 9 Amaranthus sp. 2043 PPOX 9 Amaranthus sp. 2044 PPOX 9Amaranthus sp. 2045 PPOX 9 Amaranthus sp. 2046 PPOX 9 Amaranthus sp.2047 PPOX 9 Amaranthus sp. 2048 PPOX 9 Amaranthus sp. 2049 PPOX 9Amaranthus sp. 2050 PPOX 9 Amaranthus sp. 2051 PPOX 9 Amaranthus sp.2052 PPOX 9 Amaranthus sp. 2053 PPOX 9 Amaranthus sp. 2054 PPOX 9Amaranthus sp. 2055 PPOX 9 Amaranthus sp. 2056 PPOX 9 Amaranthus sp.2057 PPOX 9 Amaranthus sp. 2058 PPOX 9 Amaranthus sp. 2059 PPOX 9Amaranthus sp. 2060 PPOX 9 Amaranthus sp. 2061 PPOX 9 Amaranthus sp.2062 PPOX 9 Amaranthus sp. 2063 PPOX 9 Amaranthus sp. 2064 PPOX 9Amaranthus sp. 2065 PPOX 9 Amaranthus sp. 2066 PPOX 9 Amaranthus sp.2067 PPOX 9 Amaranthus sp. 2068 PPOX 9 Amaranthus sp. 2069 PPOX 9Amaranthus sp. 2070 PPOX 9 Amaranthus sp. 2071 PPOX 9 Amaranthus sp.2072 PPOX 9 Amaranthus sp. 2073 PPOX 9 Amaranthus sp. 2074 PPOX 9Amaranthus sp. 2075 PPOX 9 Amaranthus sp. 2076 PPOX 9 Amaranthus sp.2077 PPOX 9 Amaranthus sp. 2078 PPOX 9 Amaranthus sp. 2079 PPOX 9Amaranthus sp. 2080 PPOX 9 Amaranthus sp. 2081 PPOX 9 Amaranthus sp.2082 PPOX 9 Amaranthus sp. 2083 PPOX 9 Amaranthus sp. 2084 PPOX 9Amaranthus sp. 2085 PPOX 9 Amaranthus sp. 2086 PPOX 9 Amaranthus sp.2087 PPOX 9 Amaranthus sp. 2088 PPOX 9 Amaranthus sp. 2089 PPOX 9Amaranthus sp. 2090 PPOX 9 Amaranthus sp. 2091 PPOX 9 Amaranthus sp.2092 PPOX 9 Amaranthus sp. 2093 PPOX 9 Amaranthus sp. 2094 PPOX 9Amaranthus sp. 2095 PPOX 9 Amaranthus sp. 2096 PPOX 9 Amaranthus sp.2097 PPOX 9 Amaranthus sp. 2098 PPOX 9 Amaranthus sp. 2099 PPOX 9Amaranthus sp. 2100 PPOX 9 Amaranthus sp. 2101 PPOX 9 Amaranthus sp.2102 PPOX 9 Amaranthus sp. 2103 PPOX 9 Amaranthus sp. 2104 PPOX 9Amaranthus sp. 2105 PPOX 9 Amaranthus sp. 2106 PPOX 9 Amaranthus sp.2107 PPOX 9 Amaranthus sp. 2108 PPOX 9 Amaranthus sp. 2109 PPOX 9Amaranthus sp. 2110 PPOX 9 Amaranthus sp. 2111 PPOX 9 Amaranthus sp.2112 PPOX 9 Amaranthus sp. 2113 PPOX 9 Amaranthus sp. 2114 PPOX 9Amaranthus sp. 2115 PPOX 9 Amaranthus sp. 2116 PPOX 9 Amaranthus sp.2117 PPOX 9 Amaranthus sp. 2118 PPOX 9 Amaranthus sp. 2119 PPOX 9Amaranthus sp. 2120 PPOX 9 Amaranthus sp. 2121 PPOX 9 Amaranthus sp.2122 PPOX 9 Amaranthus sp. 2123 PPOX 9 Amaranthus sp. 2124 PPOX 9Amaranthus sp. 2125 PPOX 9 Amaranthus sp. 2126 PPOX 9 Amaranthus sp.2127 PPOX 9 Amaranthus sp. 2128 PPOX 9 Amaranthus sp. 2129 PPOX 9Amaranthus sp. 2130 PPOX 9 Amaranthus sp. 2131 PPOX 9 Amaranthus sp.2132 PPOX 9 Amaranthus sp. 2133 PPOX 9 Amaranthus sp. 2134 PPOX 9Amaranthus sp. 2135 PPOX 9 Amaranthus sp. 2136 PPOX 9 Amaranthus sp.2137 PPOX 9 Amaranthus sp. 2138 PPOX 9 Amaranthus sp. 2139 PPOX 9Amaranthus sp. 2140 PPOX 9 Amaranthus sp. 2141 PPOX 9 Amaranthus sp.2142 PPOX 9 Amaranthus sp. 2143 PPOX 9 Amaranthus sp. 2144 PPOX 9Amaranthus sp. 2145 PPOX 9 Amaranthus sp. 2146 PPOX 9 Amaranthus sp.2147 PPOX 9 Amaranthus sp. 2148 PPOX 9 Amaranthus sp. 2149 PPOX 9Amaranthus sp. 2150 PPOX 9 Amaranthus sp. 2151 PPOX 9 Amaranthus sp.2152 PPOX 9 Amaranthus sp. 2153 PPOX 9 Amaranthus sp. 2154 PPOX 9Amaranthus sp. 2155 PPOX 9 Amaranthus sp. 2156 PPOX 9 Amaranthus sp.2157 PPOX 9 Amaranthus sp. 2158 PPOX 9 Amaranthus sp. 2159 PPOX 9Amaranthus sp. 2160 PPOX 9 Amaranthus sp. 2161 PPOX 9 Amaranthus sp.2162 PPOX 9 Amaranthus sp. 2163 PPOX 9 Amaranthus sp. 2164 PPOX 9Amaranthus sp. 2165 PPOX 9 Amaranthus sp. 2166 PPOX 9 Amaranthus sp.2167 PPOX 9 Amaranthus sp. 2168 PPOX 9 Amaranthus sp. 2169 PPOX 9Amaranthus sp. 2170 PPOX 9 Amaranthus sp. 2171 PPOX 9 Amaranthus sp.2172 PPOX 9 Amaranthus sp. 2173 PPOX 9 Amaranthus sp. 2174 PPOX 9Amaranthus sp. 2175 PPOX 9 Amaranthus sp. 2176 PPOX 9 Amaranthus sp.2177 PPOX 9 Amaranthus sp. 2178 PPOX 9 Amaranthus sp. 2179 PPOX 9Amaranthus sp. 2180 PPOX 9 Amaranthus sp. 2181 PPOX 9 Amaranthus sp.2182 PPOX 9 Amaranthus sp. 2183 PPOX 9 Amaranthus sp. 2184 PPOX 9Amaranthus sp. 2185 PPOX 9 Amaranthus sp. 2186 PPOX 9 Amaranthus sp.2187 PPOX 9 Amaranthus sp. 2188 PPOX 9 Amaranthus sp. 2189 PPOX 9Amaranthus sp. 2190 PPOX 9 Amaranthus sp. 2191 PPOX 9 Amaranthus sp.2192 PPOX 9 Amaranthus sp. 2193 PPOX 9 Amaranthus sp. 2194 PPOX 9Amaranthus sp. 2195 PPOX 9 Amaranthus sp. 2196 PPOX 9 Amaranthus sp.2197 PPOX 9 Amaranthus sp. 2198 PPOX 9 Amaranthus sp. 2199 PPOX 9Amaranthus sp. 2200 PPOX 9 Amaranthus sp. 2201 PPOX 9 Amaranthus sp.2202 PPOX 9 Amaranthus sp. 2203 PPOX 9 Amaranthus sp. 2204 PPOX 9Amaranthus sp. 2205 PPOX 9 Amaranthus sp. 2206 PPOX 9 Amaranthus sp.2207 PPOX 9 Amaranthus sp. 2208 PPOX 9 Amaranthus sp. 2209 PPOX 9Amaranthus sp. 2210 PPOX 9 Amaranthus sp. 2211 PPOX 9 Amaranthus sp.2212 PPOX 9 Amaranthus sp. 2213 PPOX 9 Amaranthus sp.

Example 3 Methods Used Related to Treating Plants or Plant Parts with aTopical Mixture of the Trigger Molecules

Glyphosate-sensitive Palmer amaranth (A. palmeri R-22) plants were grownin the greenhouse (30/20 C day/night T; 14 hour photoperiod) in 4 inchsquare pots containing Sun Gro® Redi-Earth and 3.5 kg/cubic meterOsmocote® 14-14-14 fertilizer. Palmer amaranth plants at 5 to 10 cm inheight were pre-treated with a mixture of short (24-25 mer)single-strand antisense oligo DNA polynucleotides (ssDNA) targeting PPGoxidase coding or noncoding regions at 16 nmol, formulated in 10millimolar sodium phosphate buffer (pH 6.8) containing 2% ammoniumsulfate and 0.5% Silwet L-77. Plants were treated manually by pipetting10 μL of polynucleotide solution on four fully expanded mature leaves,for a total of 40 microliters of solution per plant. Twenty-four andforty-eight hours later, the plants were treated with fomesafen (Reflex)at 22 g ai/ha and flumioxazin (Valor) at 4 g ai/ha, crop oil concentrate(COC) at 1% is added to the herbicide treatments. The formulationcontrol is the buffer and adjuvants without the DNA polynucleotides.Four replications of each treatment were conducted. Plant height isdetermined just before ssDNA treatment and at intervals up to fourteendays (dat) after herbicide treatments to determine effect of theoligonucleotide and herbicide treatments. The results shown in FIG. 1and FIG. 2 demonstrate that the oligonucleotide treatment enhanced theherbicidal activity of both fomesafen and flumioxazin.

A pool comprising eight antisense ssDNA oligonucleotides shown in Table4 was used in the protocol as described to treat palmer amaranth plantsand the individual oligonucleotides and various combinations ofoligonucleotides were tested for efficacy. Surprisingly, it wasnecessary to have a 3 oligonucleotide pool (oligo3, 5, and 7) of the 8oligonucleotides to reproduce the effect observed with the 8oligonucleotide pool and combinations of only 2 of the 3oligonucleotides was not effective to provide enhanced herbicidesensitivity. This result is illustrated in FIG. 3 for activity on Palmeramaranth. The same 3 oligonucleotides were also active on a relatedspecies, waterhemp (Amaranthus rudis).

TABLE 4  Antisense ssDNA PPG oxidase oligonucleotides OLIGO1SEQ ID NO: 2214 GTGATATTACCTCCAACACGAT OLIGO2 SEQ ID NO: 2215ATAGTAAGCACAGGATCGGAG OLIGO3 SEQ ID NO: 2216 CTTTCAATCCACTGTCAACCGOLIGO4 SEQ ID NO: 2217 ATCAAGCGTTCGAAGACCTCAT OLIGO5 SEQ ID NO: 2218CAGCAATGGCGGTAGGTAACA OLIGO6 SEQ ID NO: 2219 GCAATTGCCCGAATCCTTTTAOLIGO7 SEQ ID NO: 2220 TAGCTCAAtATCAAGGTCCTA OLIGO8 SEQ ID NO: 2221TCATAAGCACCCTCTATACAC

Example 4 A Method to Control Weeds in a Field

A method to control weeds in a field comprises the use of triggerpolynucleotides that can modulate the expression of a PPG oxidase genein one or more target weed plant species. In Table 3 (SEQ ID NOs:1381-2221), an analysis of PPG oxidase gene sequences from seventeenplant species provided a collection of 21-mer polynucleotides that canbe used in compositions to affect the growth or develop or sensitivityto PPG oxidase inhibitor herbicide to control multiple weed species in afield. A composition containing 1 or 2 or 3 or 4 or more of thepolynucleotides of Table 3 would enable broad activity of thecomposition against the multiple weed species that occur in a fieldenvironment.

The method includes creating a composition that comprises componentsthat include at least one polynucleotide of Table 3 or any othereffective gene expression modulating polynucleotide essentiallyidentical or essentially complementary to SEQ ID NO:1-71 or fragmentthereof, a transfer agent that mobilizes the polynucleotide into a plantcell and a PPG oxidase inhibiting herbicide and optionally apolynucleotide that modulates the expression of an essential gene andoptionally a herbicide that has a different mode of action relative to aPPG oxidase inhibitor. The polynucleotide of the composition includes adsRNA, ssDNA or dsDNA or a combination thereof. A composition containinga polynucleotide can have a use rate of about 1 to 30 grams or more peracre depending on the size of the polynucleotide and the number ofpolynucleotides in the composition. The composition may include one ormore additional herbicides as needed to provide effective multi-speciesweed control. A field of crop plants in need of weed plant control istreated by spray application of the composition. The composition can beprovided as a tank mix, a sequential treatment of components (generallythe polynucleotide followed by the herbicide), a simultaneous treatmentor mixing of one or more of the components of the composition fromseparate containers. Treatment of the field can occur as often as neededto provide weed control and the components of the composition can beadjusted to target specific weed species or weed families.

Example 5 Herbicidal Compositions Comprising Pesticidal Agents

A method of controlling weeds and plant pest and pathogens in a field ofPPG oxidase inhibitor tolerant crop plants is provided, wherein themethod comprises applying a composition comprising a PPG oxidase triggeroligonucleotide, a PPG oxidase inhibitor composition and an admixture ofa pest control agent. For example, the admixture comprises insecticides,fungicides, nematocides, bactericides, acaricides, growth regulators,chemosterilants, semiochemicals, repellents, attractants, pheromones,feeding stimulants or other biologically active compounds or biologicalagents, such as, microorganisms.

For example, the admixture comprises a fungicide compound for use on aPPG oxidase inhibitor tolerant crop plant to prevent or control plantdisease caused by a plant fungal pathogen. The fungicide compound of theadmixture may be a systemic or contact fungicide or mixtures of each.More particularly, the fungicide compound includes, but is not limitedto, members of the chemical groups strobilurins, triazoles,chloronitriles, carboxamides and mixtures thereof. The composition mayadditionally have an admixture comprising an insecticidal compound oragent.

The PPG oxidase trigger oligonucleotides and PPG oxidase inhibitor (forexample, fomesafen) tank mixes with fungicides, insecticides or both aretested for use in soybean and corn for control of foliar diseases andpests. Testing is conducted to develop a method for use of mixtures ofthe trigger oligonucleotides and fomesafen formulation and variouscommercially available fungicides for weed control and pest control. Thefield plots are planted with soybeans or corn. All plots receive a postplant application of the PPG oxidase trigger+fomesafen about 3 weeksafter planting. The mixtures of trigger+fomesafen ortrigger+fomesafen+fungicide+insecticides are used to treat the plots atthe R1 stage of soybean development (first flowering) or tassel stage ofcorn. Data is taken for percent weed control at 7 and 21 days after R1treatment, soybean safety (% necrosis, chlorosis, growth rate): 5 daysafter treatment, disease rating, pest ratings and yield (bushels/Acre).These mixtures and treatments are designed to provide simultaneous weedand pest control, such as fungal pest control, for example, leaf rustdisease; and insect pest control, for example, aphids, armyworms,loopers, beetles, stinkbugs, and leaf hoppers.

Agricultural chemicals are provided in containers suitable for safestorage, transportation and distribution, stability of the chemicalcompositions, mixing with solvents and instructions for use. A containerof a mixture of a trigger oligonucleotide+a herbicice+fungicidecompound, or a mixture of a trigger oligonucleotide+herbicide compoundand an insecticide compound, or a trigger oligonucleotide+a herbicidecompound and a fungicide compound and an insecticide compound (forexample, lambda-cyhalothrin, Warrier®). The container may furtherprovide instructions on the effective use of the mixture. Containers ofthe present invention can be of any material that is suitable for thestorage of the chemical mixture. Containers of the present invention canbe of any material that is suitable for the shipment of the chemicalmixture. The material can be of cardboard, plastic, metal, or acomposite of these materials. The container can have a volume of 0.5liter, 1 liter, 2 liter, 3-5 liter, 5-10 liter, 10-20 liter, 20-50 literor more depending upon the need. A tank mix of a triggeroligonucleotide+herbicide compound and a fungicide compound is provided,methods of application to the crop to achieve an effective dose of eachcompound are known to those skilled in the art and can be refined andfurther developed depending on the crop, weather conditions, andapplication equipment used.

Insecticides, fungicides, nematocides, bactericides, acaricides, growthregulators, chemosterilants, semiochemicals, repellents, attractants,pheromones, feeding stimulants or other biologically active compoundscan be added to the trigger oligonucleotide to form a multi-componentpesticide giving an even broader spectrum of agricultural protection.Examples of such agricultural protectants with which compounds of thisinvention can be formulated are: insecticides such as abamectin,acephate, azinphos-methyl, bifenthrin, buprofezin, carbofuran,chlorfenapyr, chlorpyrifos, chlorpyrifos-methyl, cyfluthrin,beta-cyfluthrin, cyhalothrin, lambda-cyhalothrin, deltamethrin,diafenthiuron, diazinon, diflubenzuron, dimethoate, esfenvalerate,fenoxycarb, fenpropathrin, fenvalerate, fipronil, flucythrinate,tau-fluvalinate, fonophos, imidacloprid, isofenphos, malathion,metaldehyde, methamidophos, methidathion, methomyl, methoprene,methoxychlor, methyl7-chloro-2,5-dihydro-2-[[N-(methoxycarbonyl)-N-[4-(trifluoromethoxy)phenyl]amino]carbonyl]indeno[1,2-e][1,3,4]oxadiazine-4a(3H)-carboxylate(DPX-JW062), monocrotophos, oxamyl, parathion, parathion-methyl,permethrin, phorate, phosalone, phosmet, phosphamidon, pirimicarb,profenofos, rotenone, sulprofos, tebufenozide, tefluthrin, terbufos,tetrachlorvinphos, thiodicarb, tralomethrin, trichlorfon andtriflumuron; most preferably a PPG oxidase inhibitor compound isformulated with a fungicide compound or combinations of fungicides, suchas azoxystrobin, benomyl, blasticidin-S, Bordeaux mixture (tribasiccopper sulfate), bromuconazole, captafol, captan, carbendazim,chloroneb, chlorothalonil, copper oxychloride, copper salts, cymoxanil,cyproconazole, cyprodinil (CGA 219417), diclomezine, dicloran,difenoconazole, dimethomorph, diniconazole, diniconazole-M, dodine,edifenphos, epoxiconazole (BAS 480F), famoxadone, fenarimol,fenbuconazole, fenpiclonil, fenpropidin, fenpropimorph, fluazinam,fluquinconazole, flusilazole, flutolanil, flutriafol, folpet,fosetyl-aluminum, furalaxyl, hexaconazole, ipconazole, iprobenfos,iprodione, isoprothiolane, kasugamycin, kresoxim-methyl, mancozeb,maneb, mepronil, metalaxyl, metconazole, S-methyl7-benzothiazolecarbothioate (CGA 245704), myclobutanil, neo-asozin(ferric methanearsonate), oxadixyl, penconazole, pencycuron,probenazole, prochloraz, propiconazole, pyrifenox, pyroquilon,quinoxyfen, spiroxamine (KWG4168), sulfur, tebuconazole, tetraconazole,thiabendazole, thiophanate-methyl, thiram, triadimefon, triadimenol,tricyclazole, trifloxystrobin, triticonazole, validamycin andvinclozolin; combinations of fungicides are common for example,cyproconazole and azoxystrobin, difenoconazole, and metalaxyl-M,fludioxonil and metalaxyl-M, mancozeb and metalaxyl-M, copper hydroxideand metalaxyl-M, cyprodinil and fludioxonil, cyproconazole andpropiconazole; commercially available fungicide formulations for controlof Asian soybean rust disease include, but are not limited to Quadris®(Syngenta Corp), Bravo® (Syngenta Corp), Echo 720® (Sipcam Agro Inc),Headline® 2.09EC (BASF Corp), Tilt® 3.6EC (Syngenta Corp), PropiMax™3.6EC (Dow AgroSciences), Bumper® 41.8EC (MakhteshimAgan), Folicur® 3.6F(Bayer CropScience), Laredo® 25EC (Dow AgroSciences), Laredo™ 25EW (DowAgroSciences), Stratego® 2.08F (Bayer Corp), Domark™ 125SL (Sipcam AgroUSA), and Pristine® 38% WDG (BASF Corp) these can be combined with PPGoxidase inhibitor compositions as described in the present invention toprovide enhanced protection from fungal disease; nematocides such asaldoxycarb and fenamiphos; bactericides such as streptomycin; acaricidessuch as amitraz, chinomethionat, chlorobenzilate, cyhexatin, dicofol,dienochlor, etoxazole, fenazaquin, fenbutatin oxide, fenpropathrin,fenpyroximate, hexythiazox, propargite, pyridaben and tebufenpyrad; andbiological agents such as Bacillus thuringiensis, Bacillus thuringiensisdelta endotoxin, baculovirus, and entomopathogenic bacteria, virus andfungi.

1-31. (canceled)
 32. A method for potentiating activity of a PPG oxidaseinhibitor herbicide in a plant comprising (a) applying to a surface ofthe plant a composition comprising at least one non-transcribablepolynucleotide and a transfer agent, wherein the non-transcribablepolynucleotide is from 18 to about 700 nucleotides in length and isidentical or complementary to at least 18 contiguous nucleotides of PPGoxidase gene sequence or an RNA transcript of the PPG oxidase genesequence, wherein the PPG oxidase gene sequence is selected from thegroup consisting of SEQ ID NOs: 7-12, 14, 15, 17, 20, 29-34, 36-57, 59,61-71, and a polynucleotide fragment thereof, and wherein the transferagent conditions the surface of the plant for permeation by thenon-transcribable polynucleotide, and (b) applying the PPG oxidaseinhibitor herbicide to the plant; whereby the non-transcribablepolynucleotide permeates the interior of the plant and inducessuppression of the PPG oxidase gene, thereby potentiating activity ofthe PPG oxidase inhibitor herbicide in the plant.
 33. The method ofclaim 32, wherein the at least one non-transcribable polynucleotide is(a) at least one polynucleotide selected from the group consisting ofsense ssDNA, anti-sense ssDNA, sense ssRNA, anti-sense ssRNA, dsRNA,dsDNA, and dsDNA/RNA hybrids; (b) at least one polynucleotide selectedfrom the group consisting of SEQ ID NOs: 72-75, 84, 85, 88, 89, 96, 97,102-105, 116, 117, 120-129, 132, 133, 138, 139, 142, 143, 146-149, 158,159, 162, 163, 166-171, 178-181, 184-425, 428-431, 436, 437, 440-523,530, 531, 534, 535, 542, 543, 548-551, 554, 555, 560-565, 568, 569,572-579, 584-587, 592-595, 602, 603, 608-611, 618, 619, 626, 627,630-733, 736-747, 750-1305, 1308-1381, and a fragment thereof; (c) atleast one polynucleotide selected from the group consisting of SEQ IDNOs: 1382-1413, 1415-1452, 1454, 1456-1465, 1467-1470, 1472-1483,1485-1523, 1525-1564, 1566-1574, 1576-1594, 1596-1602, 1604-1630,1632-1656, 1658-1676, 1678-1717, 1719-1735, 1737, 1738, 1740-1813,1815-1865, 1867-1963, 1965-1978, 1980-2010, 2012-2088, 2090, 2092-2213,and a fragment thereof; or (d) any combination of two or morenon-transcribable polynucleotides, each being from 18 to about 700nucleotides in length and is identical or complementary to at least 18contiguous nucleotides of a PPG oxidase gene sequence or an RNAtranscript of the PPG oxidase gene sequence, wherein the PPG oxidasegene sequence is selected from the group consisting of SEQ ID NOs: 7-12,14, 15, 17, 20, 29-34, 36-57, 59, 61-71, and a polynucleotide fragmentthereof.
 34. The method of claim 32, wherein the transfer agent is anorganosilicone surfactant composition or compound contained therein. 35.The method of claim 32, wherein the composition further comprises thePPG oxidase inhibitor herbicide.
 36. The method of claim 35, wherein thePPG oxidase inhibitor herbicide is selected from the group consisting ofacifluorfen-Na, bifenox, chlomethoxyfen, chlornitrofen, ethoxyfen-ethyl,fluoroglycofen-ethyl, fomesafen, halosafen, lactofen, oxyfluorfen,fluazolate, pyraflufen-ethyl, cinidon-ethyl, flumioxazin,flumiclorac-pentyl, fluthiacet-methyl, thidiazimin, oxadiazon,oxadiargyl, pyraclonil, flufenpyr-ethyl, azafenidin,carfentrazone-ethyl, Saflufenacil, sulfentrazone, pentoxazone,benzfendizone, butafenacil, pyrazogyl, and profluazol.
 37. The method ofclaim 35, wherein the composition further comprises one or moreherbicides different from the PPG oxidase inhibitor herbicide.
 38. Themethod of claim 35, wherein the composition further comprises aco-herbicide.
 39. The method of claim 38, wherein the co-herbicide isselected from the group consisting of amide herbicides, arsenicalherbicides, benzothiazole herbicides, benzoylcyclohexanedioneherbicides, benzofuranyl alkylsulfonate herbicides, cyclohexene oximeherbicides, cyclopropylisoxazole herbicides, dicarboximide herbicides,dinitroaniline herbicides, dinitrophenol herbicides, dithiocarbamateherbicides, glycine herbicides, halogenated aliphatic herbicides,imidazolinone herbicides, inorganic herbicides, nitrile herbicides,organophosphorus herbicides, oxadiazolone herbicides, oxazoleherbicides, phenoxy herbicides, phenylenediamine herbicides, pyridazineherbicides, pyridazinone herbicides, pyridine herbicides,pyrimidinediamine herbicides, pyrimidinyloxybenzylamine herbicides,quaternary ammonium herbicides, thiocarbamate herbicides, thiocarbonateherbicides, thiourea herbicides, triazine herbicides, triazinoneherbicides, triazolone herbicides, triazolopyrimidine herbicides, uracilherbicides, and urea herbicides.
 40. The method of claim 35, wherein thecomposition further comprises a pesticide.
 41. The method of claim 40,wherein the pesticide is selected from the group consisting ofinsecticides, fungicides, nematicides, bactericides, acaricides, growthregulators, chemosterilants, semiochemicals, repellents, attractants,pheromones, feeding stimulants, and biopesticides.
 42. The method ofclaim 32, wherein the plant is selected from the group consisting ofAmaranthus albus, Amaranthus graecizans, Amaranthus hybridus, Amaranthuslividus, Amaranthus palmeri, Amaranthus rudis, Amaranthus spinosus,Amaranthus thunbergii, Amaranthus viridis, Ambrosia trifida, Chenopodiumalbum, Commelina diffusa, Conyza canadensis, Digitaria sanguinalis,Euphorbia heterophylla, Kochia scoparia, and Lolium multiflorum.
 43. Amethod of reducing growth, development, or reproductive ability in aplant, comprising treating a plant with a PPG oxidase inhibitorherbicide in combination with (a) at least one non-transcribablepolynucleotide that is from 18 to about 700 nucleotides in length and isidentical or complementary to at least 18 contiguous nucleotides of aPPG oxidase gene sequence or an RNA transcript of the PPG oxidase genesequence, wherein the PPG oxidase gene sequence is selected from thegroup consisting of SEQ ID NOs: 7-12, 14, 15, 17, 20, 29-34, 36-57, 59,61-71, and a polynucleotide fragment thereof, and (b) an organosiliconesurfactant, whereby growth, development, or reproductive ability isreduced in the treated plant compared to a control plant treated withthe PPG oxidase inhibitor herbicide alone.
 44. The method of claim 43,wherein the at least one non-transcribable polynucleotide is (a) atleast one polynucleotide selected from the group consisting of sensessDNA, anti-sense ssDNA, sense ssRNA, anti-sense ssRNA, dsRNA, dsDNA,and dsDNA/RNA hybrids; (b) at least one polynucleotide selected from thegroup consisting of SEQ ID NOs: 72-75, 84, 85, 88, 89, 96, 97, 102-105,116, 117, 120-129, 132, 133, 138, 139, 142, 143, 146-149, 158, 159, 162,163, 166-171, 178-181, 184-425, 428-431, 436, 437, 440-523, 530, 531,534, 535, 542, 543, 548-551, 554, 555, 560-565, 568, 569, 572-579,584-587, 592-595, 602, 603, 608-611, 618, 619, 626, 627, 630-733,736-747, 750-1305, 1308-1381, and a fragment thereof; (c) at least onepolynucleotide selected from the group consisting of SEQ ID NOs:1382-1413, 1415-1452, 1454, 1456-1465, 1467-1470, 1472-1483, 1485-1523,1525-1564, 1566-1574, 1576-1594, 1596-1602, 1604-1630, 1632-1656,1658-1676, 1678-1717, 1719-1735, 1737, 1738, 1740-1813, 1815-1865,1867-1963, 1965-1978, 1980-2010, 2012-2088, 2090, 2092-2213, and afragment thereof; or (d) any combination of two or morenon-transcribable polynucleotides, each being from 18 to about 700nucleotides in length and identical or complementary to at least 18contiguous nucleotides of a PPG oxidase gene sequence or an RNAtranscript of the PPG oxidase gene sequence, wherein the PPG oxidasegene sequence is selected from the group consisting of SEQ ID NOs: 7-12,14, 15, 17, 20, 29-34, 36-57, 59, 61-71, and a polynucleotide fragmentthereof.
 45. The method of claim 43, wherein the PPG oxidase inhibitorherbicide is selected from the group consisting of acifluorfen-Na,bifenox, chlomethoxyfen, chlornitrofen, ethoxyfen-ethyl,fluoroglycofen-ethyl, fomesafen, halosafen, lactofen, oxyfluorfen,fluazolate, pyraflufen-ethyl, cinidon-ethyl, flumioxazin,flumiclorac-pentyl, fluthiacet-methyl, thidiazimin, oxadiazon,oxadiargyl, pyraclonil, flufenpyr-ethyl, azafenidin,carfentrazone-ethyl, Saflufenacil, sulfentrazone, pentoxazone,benzfendizone, butafenacil, pyrazogyl, and profluazol.
 46. The method ofclaim 43, wherein the composition further comprises a co-herbicide or apesticide.
 47. The method of claim 43, wherein the plant is selectedfrom the group consisting of Amaranthus albus, Amaranthus graecizans,Amaranthus hybridus, Amaranthus lividus, Amaranthus palmeri, Amaranthusrudis, Amaranthus spinosus, Amaranthus thunbergii, Amaranthus viridis,Ambrosia trifida, Chenopodium album, Commelina diffusa, Conyzacanadensis, Digitaria sanguinalis, Euphorbia heterophylla, Kochiascoparia, and Lolium multiflorum.
 48. A recombinant expression cassettecomprising a promoter functional in a microbe and operably linked to apolynucleotide that is 18 to about 700 nucleotides in length and isidentical or complementary to at least 18 contiguous nucleotides of aPPG oxidase gene sequence or an RNA transcript of the PPG oxidase genesequence, wherein the PPG oxidase gene sequence is selected from thegroup consisting of SEQ ID NOs: 7-12, 14, 15, 17, 20, 29-34, 36-57, 59,61-71, and a polynucleotide fragment thereof.
 49. A method of making arecombinant polynucleotide comprising harvesting polynucleotides from amicrobe that expresses the recombinant expression cassette of claim 48.50. An herbicidal composition comprising (a) the recombinantpolynucleotides provided by the method of claim 49, (b) anorganosilicone surfactant, and (c) a PPG oxidase inhibitor herbicide.